BMRB Entry 52368
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52368
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Title: The backbone assignment of FtsZ C-terminal disordered tail PubMed: 38890550
Deposition date: 2024-03-26 Original release date: 2024-07-22
Authors: Qu, Xiaozhan
Citation: Qu, Xiaozhan; Zhao, Shuo; Wan, Chanjuan; Zhu, Lei; Ji, Tuo; Rossi, Paolo; Wang, Junfeng; Kalodimos, Charalampos; Wang, Chao; Xu, Weiya; Huang, Chengdong. "Structural basis for the dynamic chaperoning of disordered clients by Hsp90" Nat. Struct. Mol. Biol. ., .-. (2024).
Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: TGIGMDKRPEITLVTNKQVQ
QPVMDRYQQHGMAPLTQEQK
PVAKVVNDNAPQTAKEPDYL
DIPAFLRKQAD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 117 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FtsZ C-terminal disordered tail | 1 |
Entities:
Entity 1, FtsZ C-terminal disordered tail 71 residues - Formula weight is not available
| 1 | THR | GLY | ILE | GLY | MET | ASP | LYS | ARG | PRO | GLU | ||||
| 2 | ILE | THR | LEU | VAL | THR | ASN | LYS | GLN | VAL | GLN | ||||
| 3 | GLN | PRO | VAL | MET | ASP | ARG | TYR | GLN | GLN | HIS | ||||
| 4 | GLY | MET | ALA | PRO | LEU | THR | GLN | GLU | GLN | LYS | ||||
| 5 | PRO | VAL | ALA | LYS | VAL | VAL | ASN | ASP | ASN | ALA | ||||
| 6 | PRO | GLN | THR | ALA | LYS | GLU | PRO | ASP | TYR | LEU | ||||
| 7 | ASP | ILE | PRO | ALA | PHE | LEU | ARG | LYS | GLN | ALA | ||||
| 8 | ASP |
Samples:
sample_1: FtsZ C-terminal disordered tail, [U-99% 13C; U-99% 15N], 500 uM; Phosphate 50 mM; NaCl 100 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts