BMRB Entry 52369

Name: TDP-43 W334G/W385G/W412G
Published: 2024-12-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52369

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TDP-43 W334G/W385G/W412G

Deposition date:

2024-03-27

Original release date:

2024-12-26

Authors:

Wu, Guan-Wei; Lin, Yu-Hao; Huang, Jie-rong

Citation:

Citation: Lin, Pin-Han; Wu, Guan-Wei; Lin, Yu-Hao; Huang, Jing-Rou; Jeng, U-Ser; Liu, Wei-Min; Huang, Jie-Rong. "TDP-43 Amyloid Fibril Formation via Phase Separation-Related and -Unrelated Pathways" ACS Chem. Neurosci. 15, 3767-3775 (2024).
PubMed: 39358890

Assembly members:

Assembly members:
entity_1, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MASHHHHHHGSSNRQLERSG RFGGNPGGFGNQGGFGNSRG GGAGLGNNQGSNMGGGMNFG AFSINPAMMAAAQAALQSSG GMMGMLASQQNQSGPSGNNQ NQGNMQREPNQAFGSGNNSY SGSNSGAAIGGGSASNAGSG SGFNGGFGSSMDSKSSGGGM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	406
15N chemical shifts	144
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TDP-43 D3W	1

Entities:

Entity 1, TDP-43 D3W 160 residues - Formula weight is not available

1	MET	ALA	SER	HIS	HIS	HIS	HIS	HIS	HIS	GLY
2	SER	SER	ASN	ARG	GLN	LEU	GLU	ARG	SER	GLY
3	ARG	PHE	GLY	GLY	ASN	PRO	GLY	GLY	PHE	GLY
4	ASN	GLN	GLY	GLY	PHE	GLY	ASN	SER	ARG	GLY
5	GLY	GLY	ALA	GLY	LEU	GLY	ASN	ASN	GLN	GLY
6	SER	ASN	MET	GLY	GLY	GLY	MET	ASN	PHE	GLY
7	ALA	PHE	SER	ILE	ASN	PRO	ALA	MET	MET	ALA
8	ALA	ALA	GLN	ALA	ALA	LEU	GLN	SER	SER	GLY
9	GLY	MET	MET	GLY	MET	LEU	ALA	SER	GLN	GLN
10	ASN	GLN	SER	GLY	PRO	SER	GLY	ASN	ASN	GLN
11	ASN	GLN	GLY	ASN	MET	GLN	ARG	GLU	PRO	ASN
12	GLN	ALA	PHE	GLY	SER	GLY	ASN	ASN	SER	TYR
13	SER	GLY	SER	ASN	SER	GLY	ALA	ALA	ILE	GLY
14	GLY	GLY	SER	ALA	SER	ASN	ALA	GLY	SER	GLY
15	SER	GLY	PHE	ASN	GLY	GLY	PHE	GLY	SER	SER
16	MET	ASP	SER	LYS	SER	SER	GLY	GLY	GLY	MET

Samples:

sample_1: TDP-43 D3W, [U-100% 13C; U-100% 15N], 100 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

1	MET	ALA	SER	HIS	HIS	HIS	HIS	HIS	HIS	GLY
2	SER	SER	ASN	ARG	GLN	LEU	GLU	ARG	SER	GLY
3	ARG	PHE	GLY	GLY	ASN	PRO	GLY	GLY	PHE	GLY
4	ASN	GLN	GLY	GLY	PHE	GLY	ASN	SER	ARG	GLY
5	GLY	GLY	ALA	GLY	LEU	GLY	ASN	ASN	GLN	GLY
6	SER	ASN	MET	GLY	GLY	GLY	MET	ASN	PHE	GLY
7	ALA	PHE	SER	ILE	ASN	PRO	ALA	MET	MET	ALA
8	ALA	ALA	GLN	ALA	ALA	LEU	GLN	SER	SER	GLY
9	GLY	MET	MET	GLY	MET	LEU	ALA	SER	GLN	GLN
10	ASN	GLN	SER	GLY	PRO	SER	GLY	ASN	ASN	GLN
11	ASN	GLN	GLY	ASN	MET	GLN	ARG	GLU	PRO	ASN
12	GLN	ALA	PHE	GLY	SER	GLY	ASN	ASN	SER	TYR
13	SER	GLY	SER	ASN	SER	GLY	ALA	ALA	ILE	GLY
14	GLY	GLY	SER	ALA	SER	ASN	ALA	GLY	SER	GLY
15	SER	GLY	PHE	ASN	GLY	GLY	PHE	GLY	SER	SER
16	MET	ASP	SER	LYS	SER	SER	GLY	GLY	GLY	MET

1	MET	ALA	SER	HIS	HIS	HIS	HIS	HIS	HIS	GLY
2	SER	SER	ASN	ARG	GLN	LEU	GLU	ARG	SER	GLY
3	ARG	PHE	GLY	GLY	ASN	PRO	GLY	GLY	PHE	GLY
4	ASN	GLN	GLY	GLY	PHE	GLY	ASN	SER	ARG	GLY
5	GLY	GLY	ALA	GLY	LEU	GLY	ASN	ASN	GLN	GLY
6	SER	ASN	MET	GLY	GLY	GLY	MET	ASN	PHE	GLY
7	ALA	PHE	SER	ILE	ASN	PRO	ALA	MET	MET	ALA
8	ALA	ALA	GLN	ALA	ALA	LEU	GLN	SER	SER	GLY
9	GLY	MET	MET	GLY	MET	LEU	ALA	SER	GLN	GLN
10	ASN	GLN	SER	GLY	PRO	SER	GLY	ASN	ASN	GLN
11	ASN	GLN	GLY	ASN	MET	GLN	ARG	GLU	PRO	ASN
12	GLN	ALA	PHE	GLY	SER	GLY	ASN	ASN	SER	TYR
13	SER	GLY	SER	ASN	SER	GLY	ALA	ALA	ILE	GLY
14	GLY	GLY	SER	ALA	SER	ASN	ALA	GLY	SER	GLY
15	SER	GLY	PHE	ASN	GLY	GLY	PHE	GLY	SER	SER
16	MET	ASP	SER	LYS	SER	SER	GLY	GLY	GLY	MET