BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5469

Title: Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond

Deposition date: 2002-07-22 Original release date: 2003-03-06

Authors: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji

Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji. "Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond"  Biochemistry 42, 2524-2534 (2003).

Assembly members:
ovomucoid third domain from silver pheasant, polymer, 54 residues, Formula weight is not available

Natural source:   Common Name: silver pheasant   Taxonomy ID: 9046   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lophura nycthemera

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):
ovomucoid third domain from silver pheasant: AVSVDCSEYPKPACTMEYRP LCGSDNKTYGNKCNFCNAVV ESNGTLTLSHFGKC

Data sets:
Data typeCount
1H chemical shifts343

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1omsvp3 monomer1

Entities:

Entity 1, omsvp3 monomer 54 residues - Formula weight is not available

1   ALAVALSERVALASPCYSSERGLUTYRPRO
2   LYSPROALACYSTHRMETGLUTYRARGPRO
3   LEUCYSGLYSERASPASNLYSTHRTYRGLY
4   ASNLYSCYSASNPHECYSASNALAVALVAL
5   GLUSERASNGLYTHRLEUTHRLEUSERHIS
6   PHEGLYLYSCYS

Samples:

sample_1: ovomucoid third domain from silver pheasant 4.0 mM

Ex-cond_1: pH: 4.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQF-COSYnot availablenot availablenot available

Software:

NMRPipe - processing

NMRPIPP - assignment, peak picking

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 1374 1375 4068 42 4864 4865 507 5411 5424 5425 5426 5427 5428 5429 5448 5518 5520
PDB
PIR A31445 B61588 C31438 E31437 E31442
SWISS-PROT P52245 P52258 P52261 P52269 P67944