BMRB Entry 5903
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5903
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Title: 1H,13C and 15N backbone resonance assignment of the Hyaluronan-binding domain of CD44 PubMed: 15017146
Deposition date: 2003-08-15 Original release date: 2004-04-07
Authors: Takeda, Mitsuhiro; Terasawa, Hiroaki; Sakakura, Masayosh; Yamaguchi, Yoshiki; Kajiwara, Masahiro; Kawashima, Hiroto; Miyasaka, Masayuki; Shimada, Ichio
Citation: Takeda, Mitsuhiro; Terasawa, Hiroaki; Sakakura, Masayoshi; Yamaguchi, Yoshiki; Kajiwara, Masahiro; Kawashima, Hiroto; Miyasaka, Masayuki; Shimada, Ichio. "Letter to the Editor: 1H, 13C and 15N backbone resonance assignments of the Hyaluronan-binding domain of CD44" J. Biomol. NMR 29, 97-98 (2004).
Assembly members:
CD44 hyaluronan-binding domain, polymer, 160 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CD44 hyaluronan-binding domain: MAQIDLNITCRFAGVFHVEK
NGRYSISRTEAADLCKAFNS
TLPTMAQMEKALSIGFETCR
YGFIEGHVVIPRIHPNSICA
ANNTGVYILTSNTSQYDTYC
FNASAPPEEDCTSVTDLPNA
FDGPITITIVNRDGTRYVQK
GEYRTNPEDIYPSNPTDDDV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 284 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD44 hyaluronan binding domain | 1 |
Entities:
Entity 1, CD44 hyaluronan binding domain 160 residues - Formula weight is not available
| 1 | MET | ALA | GLN | ILE | ASP | LEU | ASN | ILE | THR | CYS | |
| 2 | ARG | PHE | ALA | GLY | VAL | PHE | HIS | VAL | GLU | LYS | |
| 3 | ASN | GLY | ARG | TYR | SER | ILE | SER | ARG | THR | GLU | |
| 4 | ALA | ALA | ASP | LEU | CYS | LYS | ALA | PHE | ASN | SER | |
| 5 | THR | LEU | PRO | THR | MET | ALA | GLN | MET | GLU | LYS | |
| 6 | ALA | LEU | SER | ILE | GLY | PHE | GLU | THR | CYS | ARG | |
| 7 | TYR | GLY | PHE | ILE | GLU | GLY | HIS | VAL | VAL | ILE | |
| 8 | PRO | ARG | ILE | HIS | PRO | ASN | SER | ILE | CYS | ALA | |
| 9 | ALA | ASN | ASN | THR | GLY | VAL | TYR | ILE | LEU | THR | |
| 10 | SER | ASN | THR | SER | GLN | TYR | ASP | THR | TYR | CYS | |
| 11 | PHE | ASN | ALA | SER | ALA | PRO | PRO | GLU | GLU | ASP | |
| 12 | CYS | THR | SER | VAL | THR | ASP | LEU | PRO | ASN | ALA | |
| 13 | PHE | ASP | GLY | PRO | ILE | THR | ILE | THR | ILE | VAL | |
| 14 | ASN | ARG | ASP | GLY | THR | ARG | TYR | VAL | GLN | LYS | |
| 15 | GLY | GLU | TYR | ARG | THR | ASN | PRO | GLU | ASP | ILE | |
| 16 | TYR | PRO | SER | ASN | PRO | THR | ASP | ASP | ASP | VAL |
Samples:
Sample_1: CD44 hyaluronan-binding domain, [U-98% 13C; U-98% 15N], 2.0 mM
EX-condition: pH: 6.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts