BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6259

Title: 1H, 13C, and 15N chemical shift assignments for GatB (backbone + side chains)   PubMed: 16963640

Deposition date: 2004-07-02 Original release date: 2006-10-02

Authors: VOLPON, Laurent; YOUNG, Chris; GEHRING, Kalle

Citation: Volpon, Laurent; Young, Chris; Matte, A.; Gehring, Kalle. "NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA."  Protein Sci. 15, 2435-2441 (2006).

Assembly members:
GatB, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
GatB: MGSSHHHHHHHHENLYFQGS KRKIIVACGGAVATSTMAAE EIKELCQSHNIPVELIQCRV NEIETYMDGVHLICTTARVD RSFGDIPLVHGMPFVSGVGI EALQNKILTILQG

Data sets:
Data typeCount
13C chemical shifts412
1H chemical shifts614
15N chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GatB monomer1

Entities:

Entity 1, GatB monomer 113 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   HISHISGLUASNLEUTYRPHEGLNGLYSER
3   LYSARGLYSILEILEVALALACYSGLYGLY
4   ALAVALALATHRSERTHRMETALAALAGLU
5   GLUILELYSGLULEUCYSGLNSERHISASN
6   ILEPROVALGLULEUILEGLNCYSARGVAL
7   ASNGLUILEGLUTHRTYRMETASPGLYVAL
8   HISLEUILECYSTHRTHRALAARGVALASP
9   ARGSERPHEGLYASPILEPROLEUVALHIS
10   GLYMETPROPHEVALSERGLYVALGLYILE
11   GLUALALEUGLNASNLYSILELEUTHRILE
12   LEUGLNGLY

Samples:

sample_1: GatB, [U-13C; U-15N], 1.0 mM; KCl 75 mM; NaCl 75 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; pressure: 1 atm; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
(HCCH) TOCSYsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
2D NOESYsample_1not availableEx-cond_1

Software:

Sparky v3.106 -

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAB36319 BAG77886 BAH46969 BAI31343 BAI55515
EMBL CAA56229 CAR03521 CAR13613 CAR17061 CAS09786
GB AAG57150 AAN43688 AAN81074 AAP17514 ABB62344
PRF 2113201D
REF NP_288595 NP_310923 NP_707981 NP_754506 NP_837705
SP P0A435 P0A436 P0A437
AlphaFold P0A436 P0A435 P0A437

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts