BMRB Entry 6260
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6260
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Title: NMR solution Structure of a Highly Stable de novo Heterodimeric Coiled-Coil
Deposition date: 2004-07-12 Original release date: 2004-07-26
Authors: Lindhout, Darrin; Litowski, Jennifer; Mercier, Pascal; Hodges, Robert; Sykes, Brian
Citation: Lindhout, Darrin; Litowski, Jennifer; Mercier, Pascal; Hodges, Robert; Sykes, Brian. "NMR solution Structure of a Highly Stable de novo Heterodimeric Coiled-Coil" Biopolymers ., .-..
Assembly members:
IAAL-E3, polymer, 21 residues, Formula weight is not available
K3 coiled-coil, polymer, 21 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
IAAL-E3: EIAALEKEIAALEKEIAALE
K
K3 coiled-coil: KIAALKEKIAALKEKIAALK
E
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 42 |
| 1H chemical shifts | 240 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IAAL-E3 | 1 |
| 2 | K3 coiled-coil | 2 |
Entities:
Entity 1, IAAL-E3 21 residues - Formula weight is not available
| 1 | GLU | ILE | ALA | ALA | LEU | GLU | LYS | GLU | ILE | ALA | ||||
| 2 | ALA | LEU | GLU | LYS | GLU | ILE | ALA | ALA | LEU | GLU | ||||
| 3 | LYS |
Entity 2, K3 coiled-coil 21 residues - Formula weight is not available
| 1 | LYS | ILE | ALA | ALA | LEU | LYS | GLU | LYS | ILE | ALA | ||||
| 2 | ALA | LEU | LYS | GLU | LYS | ILE | ALA | ALA | LEU | LYS | ||||
| 3 | GLU |
Samples:
sample_1: IAAL-E3 1.0 mM; K3 coiled-coil 1.0 mM
experimental: ionic strength: 150 mM; pH: 6.70; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
Software:
VNMR -
CYANA -
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
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