BMRB Entry 6289
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6289
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Title: NMR Structure of the Free Zinc Binding C-terminal Domain of SecA PubMed: 15488768
Deposition date: 2004-08-13 Original release date: 2004-09-01
Authors: Matousek, William; Alexandrescu, Andrei
Citation: Matousek, William; Alexandrescu, Andrei. "NMR structure of the C-terminal domain of SecA in the free state." Biochim. Biophys. Acta 1702, 163-171 (2004).
Assembly members:
SecA_Ct, polymer, 22 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SecA_Ct: GRNDPCPCGSGKKYKQCHGR
LQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 55 |
| 15N chemical shifts | 18 |
| 1H chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SecA_Ct | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, SecA_Ct 22 residues - Formula weight is not available
| 1 | GLY | ARG | ASN | ASP | PRO | CYS | PRO | CYS | GLY | SER | ||||
| 2 | GLY | LYS | LYS | TYR | LYS | GLN | CYS | HIS | GLY | ARG | ||||
| 3 | LEU | GLN |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: SecA_Ct 2.5 mM; zinc chloride 2.7 mM; potassium phosphate 10 mM; H2O 90%; D2O 10%
sample_condition_1: ionic strength: 0.01 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
sample_condition_2: ionic strength: 0.01 M; pH: 6.03; pressure: 1 atm; temperature: 298 K
sample_condition_3: ionic strength: 0.01 M; pH: 6.80; pressure: 1 atm; temperature: 298 K
sample_condition_4: ionic strength: 0.01 M; pH: 6.43; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H NOESY | sample_1 | not available | not available |
| 2D 1H TOCSY | sample_1 | not available | not available |
| natural abundance 1H15N HSQC | sample_1 | not available | not available |
| 1D hydrogen exchange | sample_1 | not available | not available |
| natural abundance 1H13C HSQC | sample_1 | not available | not available |
| 1H 2D ECOSY | sample_1 | not available | not available |
| 1H 2D phase sensitive COSY | sample_1 | not available | not available |
Software:
VNMR -
FELIX v2000 -
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| SP | A4G8S7 A4TQ74 A1JJK2 A2SJF9 A1A7E3 |
| REF | NP_454748 NP_308129 NP_414640 AP_000761 NP_285794 |
| GB | AAM87165 AAG54402 AAL19100 AAA24619 AAC73209 |
| PIR | AE0519 |
| EMBL | CAH19937 CAL10790 CAD01293 CAG76705 CAA38875 |
| DBJ | BAI23462 BAG75624 BAH61745 BAB33525 BAB96666 |
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