BMRB Entry 6499
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6499
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Title: NMR structure of antimicrobial peptide distinctin in water PubMed: 15840728
Deposition date: 2005-02-09 Original release date: 2005-05-26
Authors: Amodeo, P.; Raimondo, D.; Andreotti, G.; Motta, A.; Scaloni, A.
Citation: Raimondo, D.; Andreotti, G.; Saint, N.; Amodeo, P.; Renzone, G.; Sanseverino, M.; Zocchi, I.; Molle, G.; Motta, A.; Scaloni, A.. "A folding-dependent mechanism of antimicrobial peptide resistance to degradation unveiled by solution structure of distinctin." Proc. Natl. Acad. Sci. U. S. A. ., .-..
Assembly members:
Distinctin chain A, polymer, 22 residues, Formula weight is not available
Distinctin chain B, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: tree frog Taxonomy ID: 164618 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Phyllomedusa distincta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Distinctin chain A: ENREVPPGFTALIKTLRKCK
II
Distinctin chain B: NLVSGLIEARKYLEQLHRKL
KNCKV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 413 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Distinctin chain A monomer 1 | 1 |
| 2 | Distinctin chain B monomer 1 | 2 |
| 3 | Distinctin chain A monomer 2 | 1 |
| 4 | Distinctin chain B monomer 2 | 2 |
Entities:
Entity 1, Distinctin chain A monomer 1 22 residues - Formula weight is not available
| 1 | GLU | ASN | ARG | GLU | VAL | PRO | PRO | GLY | PHE | THR | ||||
| 2 | ALA | LEU | ILE | LYS | THR | LEU | ARG | LYS | CYS | LYS | ||||
| 3 | ILE | ILE |
Entity 2, Distinctin chain B monomer 1 25 residues - Formula weight is not available
| 1 | ASN | LEU | VAL | SER | GLY | LEU | ILE | GLU | ALA | ARG | ||||
| 2 | LYS | TYR | LEU | GLU | GLN | LEU | HIS | ARG | LYS | LEU | ||||
| 3 | LYS | ASN | CYS | LYS | VAL |
Samples:
sample_1: Distinctin chain A0.05 – 3.8 mM; Distinctin chain B0.05 – 3.8 mM; Phosphate 20 mM; NaCl 100 mM
sample_cond_1: ionic strength: 0.15 M; pH: 5.8; pressure: 1 atm; temperature: 300 K
sample_cond_2: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
AMBER v5 - refinement, structure solution
Molmol v2K.2 - data analysis
xwinnmr v3.5 - processing
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |