BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6609

Title: Solution structure of the ubiquitin-like domain of human DC-UBP from dendritic cells   PubMed: 15987890

Deposition date: 2005-04-26 Original release date: 2005-09-22

Authors: Gao, Yong-Guang; Song, Ai-Xin; Shi, Yan-Hong; Chang, Yong-Ghang; Lin, Dong-Hai; Hu, Hong-Yu

Citation: Gao, Yong-Guang; Song, Ai-Xin; Shi, Yan-Hong; Chang, Yong-Ghang; Liu, Shu-Xun; Yu, Yi-Zi; Cao, Xue-Tao; Lin, Dong-Hai; Hu, Hong-Yu. "Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells."  Protein Sci. 14, 2044-2050 (2005).

Assembly members:
dendritic cell-derived ubiquitin-like protein, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
dendritic cell-derived ubiquitin-like protein: MIEEKSDIETLDIPEPPPNS GYECQLRLRLSTGKDLKLVV RSTDTVFHMKRRLHAAEGVE PGSQRWFFSGRPLTDKMKFE ELKIPKDYVVQVIVSQPVQN PTPVENLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts350
13C chemical shifts176
15N chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dendritic cell-derived ubiquitin-like protein1

Entities:

Entity 1, dendritic cell-derived ubiquitin-like protein 114 residues - Formula weight is not available

1   METILEGLUGLULYSSERASPILEGLUTHR
2   LEUASPILEPROGLUPROPROPROASNSER
3   GLYTYRGLUCYSGLNLEUARGLEUARGLEU
4   SERTHRGLYLYSASPLEULYSLEUVALVAL
5   ARGSERTHRASPTHRVALPHEHISMETLYS
6   ARGARGLEUHISALAALAGLUGLYVALGLU
7   PROGLYSERGLNARGTRPPHEPHESERGLY
8   ARGPROLEUTHRASPLYSMETLYSPHEGLU
9   GLULEULYSILEPROLYSASPTYRVALVAL
10   GLNVALILEVALSERGLNPROVALGLNASN
11   PROTHRPROVALGLUASNLEUGLUHISHIS
12   HISHISHISHIS

Samples:

sample_1: dendritic cell-derived ubiquitin-like protein, [U-15N], 1.2 mM; NaAc 10 mM; NaCl 100 mM; DTT 5 mM; sodium azide 0.02%; H2O 92%; D2O 8%

sample_2: dendritic cell-derived ubiquitin-like protein, [U-15N; U-13C], 1.2 mM; NaAc 10 mM; NaCl 100 mM; DTT 5 mM; sodium azide 0.02%; D2O 100%

sample_cond_1: pH: 5.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

XWINNMR - collection

NMRPipe - processing

nmrview v5.0 - data analysis

CNS v1.1 - refinement

aria v1.2 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG36083 BAG51130
GB AAH19910 AAL99389 AIC61103 EAW61436 EAW61437
REF NP_001181019 NP_689490 XP_002744600 XP_002816238 XP_003273289
SP Q8WUN7
AlphaFold Q8WUN7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts