BMRB Entry 6916
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6916
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Title: NMR structure of the C-terminal domain (dimer) of HPV45 oncoprotein E7 PubMed: 16636661
Deposition date: 2005-12-05 Original release date: 2007-11-15
Authors: Ohlenschlager, O.; Gorlach, M.
Citation: Ohlenschlager, O.; Seiboth, T.; Zengerling, H.; Briese, L.; Marchanka, A.; Ramachandran, R.; Baum, M.; Korbas, M.; Meyer-Klaucke, W.; Durst, M.; Gorlach, M.. "Solution structure of the partially folded high-risk human papilloma virus 45 oncoprotein E7" Oncogene 25, 5953-5959 (2006).
Assembly members:
Protein E7, polymer, 56 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human papillomavirus Taxonomy ID: 10566 Superkingdom: Viruses Kingdom: not available Genus/species: not available Human papillomavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Protein E7: GSHMAEPQRHKILCVCCKCD
GRIELTVESSAEDLRTLQQL
FLSTLSFVCPWCATNQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 56 |
| 1H chemical shifts | 380 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Protein E7, chain 1 | 1 |
| 2 | Protein E7, chain 2 | 1 |
| 3 | ZINC ION, 1 | 2 |
| 4 | ZINC ION, 2 | 2 |
Entities:
Entity 1, Protein E7, chain 1 56 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ALA | GLU | PRO | GLN | ARG | HIS | ||||
| 2 | LYS | ILE | LEU | CYS | VAL | CYS | CYS | LYS | CYS | ASP | ||||
| 3 | GLY | ARG | ILE | GLU | LEU | THR | VAL | GLU | SER | SER | ||||
| 4 | ALA | GLU | ASP | LEU | ARG | THR | LEU | GLN | GLN | LEU | ||||
| 5 | PHE | LEU | SER | THR | LEU | SER | PHE | VAL | CYS | PRO | ||||
| 6 | TRP | CYS | ALA | THR | ASN | GLN |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Protein E7, [U-15N], 1 mM; d-TRIS 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_2: Protein E7, [U-13C; U-15N], 1 mM; d-TRIS 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 120 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
Software:
VNMR v6.1 rev. C - processing
XEASY v1.3.9 - data analysis
CYANA v2.1 - structure solution
OPAL v2.6 - refinement
NMR spectrometers:
- Varian INOVA 750 MHz
Related Database Links:
| SWISS-PROT | P21736 |
| GenBank | ABP99833 ABP99825 ABP99817 ABP99809 AAA46974 |
| EMBL | CAB44707 CAA73661 CAA52574 |
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts