BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7064

Title: Cycloviolacin O14   PubMed: 16872274

Deposition date: 2006-04-10 Original release date: 2008-07-07

Authors: Ireland, D.; Colgrave, M.; Craik, D.

Citation: Ireland, D.; Colgrave, M.; Craik, D.. "A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability"  Biochem. J. 400, 1-12 (2006).

Assembly members:
Cycloviolacin O14, polymer, 31 residues, Formula weight is not available

Natural source:   Common Name: Sweet violet   Taxonomy ID: 97441   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viola odorata

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Cycloviolacin O14: GSIPACGESCFKGKCYTPGC SCSKYPLCAKN

Data sets:
Data typeCount
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cycloviolacin O141

Entities:

Entity 1, Cycloviolacin O14 31 residues - Formula weight is not available

1   GLYSERILEPROALACYSGLYGLUSERCYS
2   PHELYSGLYLYSCYSTYRTHRPROGLYCYS
3   SERCYSSERLYSTYRPROLEUCYSALALYS
4   ASN

Samples:

sample_1: Cycloviolacin O14 1.5 mM; H2O 90%; D2O 10%

sample_2: Cycloviolacin O14 1.5 mM; D2O 100%

sample_cond_1: ionic strength: 1 mM; pH: 3.5; pressure: 1 atm; temperature: 290 K

sample_cond_2: ionic strength: 1 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

DIANA v1.5 - refinement

CNS v1.1 - refinement

SPARKY v3.110 - processing

NMR spectrometers:

  • Bruker ARX 600 MHz