BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7266

Title: Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5

Deposition date: 2006-08-15 Original release date: 2006-09-05

Authors: Cort, John; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal

Citation: Cort, John; Ramelot, Theresa; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal. "Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5"  The BMRB entry is the only known published source for the data..

Assembly members:
NE1680, polymer, 109 residues, Formula weight is not available

Natural source:   Common Name: Nitrosomonas europaea   Taxonomy ID: 915   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Nitrosomonas europaea

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NE1680: MGSSHHHHHHSSGRENLYFQ GHMQIHVYDTYVKAKDGHVM HFDVFTDVRDDKKAIEFAKQ WLSSIGEEGATVTSEECRFC HSEKAPDEVIEAIKQNGYFI YKMEGCNGS

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts87
1H chemical shifts569

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NE16801

Entities:

Entity 1, NE1680 109 residues - Formula weight is not available

The construct has a non-native 22-residue N-term tag sequence (MGSSHHHHHHSSGRENLYFQGH), and a two-residue C-term cloning artifact (GS).

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETGLNILEHISVALTYRASPTHR
4   TYRVALLYSALALYSASPGLYHISVALMET
5   HISPHEASPVALPHETHRASPVALARGASP
6   ASPLYSLYSALAILEGLUPHEALALYSGLN
7   TRPLEUSERSERILEGLYGLUGLUGLYALA
8   THRVALTHRSERGLUGLUCYSARGPHECYS
9   HISSERGLULYSALAPROASPGLUVALILE
10   GLUALAILELYSGLNASNGLYTYRPHEILE
11   TYRLYSMETGLUGLYCYSASNGLYSER

Samples:

sample_1: NE1680, [U-13C; U-15N], 1 ± 0.2 mM; NaCl 450 ± 10 mM; MOPS 10 ± 1 mM; sodium azide 0.01 ± 0.002 w/v %; H2O 90 v/v %; D2O 10 v/v %

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13C HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
CC-NH-TOCSYsample_1not availableconditions_1
HC-NH-NOCSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNHAsample_1not availableconditions_1
HBCBCGCDHDsample_1not availableconditions_1
CBCACOCAHAsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
EMBL CAD85591
REF NP_841712 WP_011112234

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts