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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7323
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Meenana, Nicola; Sharmaa, Amit; Fleishmanb, Sarel; MacDonaldc, Colin; Morelc, Bertrand; Boetzelc, Ruth; Moorec, Geoffrey; Bakerb, David; Kleanthousa, Colin. "The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction" Proc. Natl. Acad. Sci. U S A. 107, 10080-10085.
PubMed: 20479265
Assembly members:
IM2, polymer, 86 residues, 9995 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichica coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
IM2: MELKHSISDYTEAEFLEFVK
KICRAEGATEEDDNKLVREF
ERLTEHPDGSDLIYYPRDDR
EDSPEGIVKEIKEWRAANGK
SGFKQG
Data type | Count |
13C chemical shifts | 301 |
15N chemical shifts | 85 |
1H chemical shifts | 588 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Colicin immunity protein IM2 | 1 |
Entity 1, Colicin immunity protein IM2 86 residues - 9995 Da.
1 | MET | GLU | LEU | LYS | HIS | SER | ILE | SER | ASP | TYR | ||||
2 | THR | GLU | ALA | GLU | PHE | LEU | GLU | PHE | VAL | LYS | ||||
3 | LYS | ILE | CYS | ARG | ALA | GLU | GLY | ALA | THR | GLU | ||||
4 | GLU | ASP | ASP | ASN | LYS | LEU | VAL | ARG | GLU | PHE | ||||
5 | GLU | ARG | LEU | THR | GLU | HIS | PRO | ASP | GLY | SER | ||||
6 | ASP | LEU | ILE | TYR | TYR | PRO | ARG | ASP | ASP | ARG | ||||
7 | GLU | ASP | SER | PRO | GLU | GLY | ILE | VAL | LYS | GLU | ||||
8 | ILE | LYS | GLU | TRP | ARG | ALA | ALA | ASN | GLY | LYS | ||||
9 | SER | GLY | PHE | LYS | GLN | GLY |
sample_1: IM2, [U-100% 13C; U-100% 15N], 1.8 mM; Sodium Azide 14 mM; DTT 5 mM; Potassium Phosphate 50 mM
conditions_1: pH: 6.2; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H15N_HSQC | sample_1 | not available | conditions_1 |
CBCA(CO)NH | sample_1 | not available | conditions_1 |
HBHA(CBCACO)NH | sample_1 | not available | conditions_1 |
HCCH-TOCSY | sample_1 | not available | conditions_1 |
HNCACB | sample_1 | not available | conditions_1 |
HN(CA)HA | sample_1 | not available | conditions_1 |
15N_NOESYHSQC | sample_1 | not available | conditions_1 |
13C_NOESYHSQC | sample_1 | not available | conditions_1 |
No software information available
PDB | |
EMBL | CAA25610 CAA26146 |
GB | AAA23069 AAN28374 ABI95491 ACH81154 EDV85355 |
REF | WP_000420691 WP_000420692 WP_000420693 WP_024222287 WP_032311204 |
SP | P04482 |
AlphaFold | P04482 |
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