BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7323

Title: 1H, 13C and 15N nmr chemical shift assignments for the colicin immunity protein IM2   PubMed: 20479265

Deposition date: 2006-10-24 Original release date: 2011-05-27

Authors: Boetzel, Ruth; Morel, Bertrand; Macdonald, Colin; Le Duff, Cecile; Spronk, Chris; Bonvin, Alexandre; James, Richard; Kleanthous, Colin; Moore, Geoffrey

Citation: Meenana, Nicola; Sharmaa, Amit; Fleishmanb, Sarel; MacDonaldc, Colin; Morelc, Bertrand; Boetzelc, Ruth; Moorec, Geoffrey; Bakerb, David; Kleanthousa, Colin. "The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction"  Proc. Natl. Acad. Sci. U S A. 107, 10080-10085.

Assembly members:
IM2, polymer, 86 residues, 9995 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichica coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
IM2: MELKHSISDYTEAEFLEFVK KICRAEGATEEDDNKLVREF ERLTEHPDGSDLIYYPRDDR EDSPEGIVKEIKEWRAANGK SGFKQG

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts85
1H chemical shifts588

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Colicin immunity protein IM21

Entities:

Entity 1, Colicin immunity protein IM2 86 residues - 9995 Da.

1   METGLULEULYSHISSERILESERASPTYR
2   THRGLUALAGLUPHELEUGLUPHEVALLYS
3   LYSILECYSARGALAGLUGLYALATHRGLU
4   GLUASPASPASNLYSLEUVALARGGLUPHE
5   GLUARGLEUTHRGLUHISPROASPGLYSER
6   ASPLEUILETYRTYRPROARGASPASPARG
7   GLUASPSERPROGLUGLYILEVALLYSGLU
8   ILELYSGLUTRPARGALAALAASNGLYLYS
9   SERGLYPHELYSGLNGLY

Samples:

sample_1: IM2, [U-100% 13C; U-100% 15N], 1.8 mM; Sodium Azide 14 mM; DTT 5 mM; Potassium Phosphate 50 mM

conditions_1: pH: 6.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HBHA(CBCACO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CA)HAsample_1not availableconditions_1
15N_NOESYHSQCsample_1not availableconditions_1
13C_NOESYHSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
EMBL CAA25610 CAA26146
GB AAA23069 AAN28374 ABI95491 ACH81154 EDV85355
REF WP_000420691 WP_000420692 WP_000420693 WP_024222287 WP_032311204
SP P04482
AlphaFold P04482

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts