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BMRB Entry 7397 BMRB - Biological Magnetic Resonance Bank
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Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7397

Title: Purification and structural characterization of a D-amino acid containing conopeptide, marmophine, from Conus marmoreus   PubMed: 18355315

Deposition date: 2007-06-29 Original release date: 2008-06-17

Authors: Huang, F.; Du, W.; Han, Y.; Wang, C.

Citation: Han, Y.; Huang, F.; Jiang, H.; Liu, L.; Wang, Q.; Wang, Y.; Shao, X.; Chi, C.; Du, W.; Wang, C.. "Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus"  FEBS J. 275, 1976-1987 (2008).

Assembly members:
M-conotoxin_mr12, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 623   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
M-conotoxin_mr12: DWEYHAHPKXNSXWT

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M-conotoxin_mr121

Entities:

Entity 1, M-conotoxin_mr12 15 residues - Formula weight is not available

1   ASPTRPGLUTYRHISALAHISPROLYSHYP
2   ASNSERDPNTRPTHR

Samples:

sample_1: M-conotoxin_mr12 2.0 mM; H2O 90%; D2O 10%

sample_2: M-conotoxin_mr12 2.0 mM; D2O 99.99%

sample_conditions_1: pH: 3.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availableisotropicsample_conditions_1
2D TOCSYnot availableisotropicsample_conditions_1
DQF-COSYnot availableisotropicsample_conditions_1
2D NOESYnot availableisotropicsample_conditions_1
2D TOCSYnot availableisotropicsample_conditions_1
DQF-COSYnot availableisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Inc. - collection

TOPSPIN v1.3B, Bruker Inc. - processing

SPARKY v3.110, T. D. Goddard and D. G. Kneller - structure solution

CYANA v2.1, L. A. Systems, Inc. - structure solution

AMBER v9.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz