BMRB Entry 12041

Name: 1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei
Published: 2020-07-09

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR12041

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei PubMed: 32488600

Deposition date: 2020-01-15 Original release date: 2020-07-09

Authors: Wang, Rui; Gao, Jie; Liao, Shanhui

Citation: Wang, Rui; Zhang, Jiahai; Liao, Shanhui; Tu, Xiaoming. "1H, 13C and 15N resonance assignments of Q38FZ4, hypothetical protein from Trypanosoma brucei" Biomol. NMR Assign. ., .-. (2020).

Assembly members:
Q38FZ4, polymer, 93 residues, Formula weight is not available

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22(b)

Entity Sequences (FASTA):
Q38FZ4: MAPAVIEIHIPLDRIRNEEY ATDDLLLNCLSKIGDTPEED GLPLRTWILREAHQALIKSP KLRTVLVKPQTVKDKPTHFQ ICFDELEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	259
15N chemical shifts	91
1H chemical shifts	601

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Q38FZ4	1

Entities:

Entity 1, Q38FZ4 93 residues - Formula weight is not available

1

MET

ALA

PRO

ALA

VAL

ILE

GLU

ILE

HIS

ILE

2

PRO

LEU

ASP

ARG

ILE

ARG

ASN

GLU

TYR

3

ALA

THR

ASP

LEU

ASN

CYS

LEU

4

SER

LYS

ILE

GLY

ASP

THR

PRO

GLU

ASP

5

GLY

LEU

PRO

LEU

ARG

THR

TRP

ILE

LEU

ARG

6

GLU

ALA

HIS

GLN

ALA

LEU

ILE

LYS

SER

PRO

7

LYS

LEU

ARG

THR

VAL

LEU

VAL

LYS

PRO

GLN

8

THR

VAL

LYS

ASP

LYS

PRO

THR

HIS

PHE

GLN

9

ILE

CYS

PHE

ASP

GLU

LEU

GLU

HIS

10

HIS

Samples:

sample_1: Q38FZ4, [U-99% 13C; U-99% 15N], 0.8 mM; NaH2PO4 25 mM; NaCl 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts