BMRB Entry 15346
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15346
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Title: NMR chemical shift assignments for E. coli YejL protein: target ER309 of the Northeast Structural Genomics Consortium
Deposition date: 2007-06-29 Original release date: 2007-07-25
Authors: Cort, John; Zhao, Li; Jiang, Mei; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Kennedy, Michael
Citation: Cort, John; Montelione, Gaetano; Acton, Thomas; Kennedy, Michael. "NMR Chemical Shift Assignments of E. coli YejL protein" .
Assembly members:
YejL, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21
Entity Sequences (FASTA):
YejL: MPQISRYSDEQVEQLLAELL
NVLEKHKAPTDLSLMVLGNM
VTNLINTSIAPAQRQAIANS
FARALQSSINEDKAHLEHHH
HHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 360 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 562 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YejL, subunit A | 1 |
| 2 | YejL, subunit B | 1 |
Entities:
Entity 1, YejL, subunit A 83 residues - Formula weight is not available
| 1 | MET | PRO | GLN | ILE | SER | ARG | TYR | SER | ASP | GLU | ||||
| 2 | GLN | VAL | GLU | GLN | LEU | LEU | ALA | GLU | LEU | LEU | ||||
| 3 | ASN | VAL | LEU | GLU | LYS | HIS | LYS | ALA | PRO | THR | ||||
| 4 | ASP | LEU | SER | LEU | MET | VAL | LEU | GLY | ASN | MET | ||||
| 5 | VAL | THR | ASN | LEU | ILE | ASN | THR | SER | ILE | ALA | ||||
| 6 | PRO | ALA | GLN | ARG | GLN | ALA | ILE | ALA | ASN | SER | ||||
| 7 | PHE | ALA | ARG | ALA | LEU | GLN | SER | SER | ILE | ASN | ||||
| 8 | GLU | ASP | LYS | ALA | HIS | LEU | GLU | HIS | HIS | HIS | ||||
| 9 | HIS | HIS | HIS |
Samples:
sample_1: YejL, [U-100% 13C; U-100% 15N], 1.5 ± 0.2 mM; D2O 5 ± 0.1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM
sample_2: YejL, [U-100% 13C; U-100% 15N], 1.5 ± 0.2 mM; D2O 100 ± 0.1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM
sample_3: YejL, [5% 13C; U-100% 15N], 1.5 ± .2 mM; D2O 5 ± .1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM
sample_conditions_1: ionic strength: 130 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 4d 1H-13C-13C-1H HMQC NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C edited-filtered NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection
FELIX v97, Accelrys Software Inc. - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 600 MHz
- Varian INOVA 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAB36502 BAE76652 BAG77980 BAI26312 BAI31437 |
| EMBL | CAP76689 CAQ32593 CAQ99114 CAR03618 CAR08819 |
| GB | AAA16383 AAC75248 AAG57325 AAN43793 AAN81179 |
| REF | NP_311106 NP_416692 NP_708086 WP_001135663 WP_001135664 |
| SP | A7ZP12 A8A251 B1IY82 B1LKT7 B1X885 |
| AlphaFold | A7ZP12 A8A251 B1IY82 B1LKT7 B1X885 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts