BMRB Entry 15738
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15738
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Carbon storage regulator from Helicobacter pylori
Deposition date: 2008-04-21 Original release date: 2014-03-05
Authors: Yang, Chun-Jun; Chen, Heng-Li; Cheng, Hsi-Tsung; Chiu, Chui-Lin; Cheng, Jya-Wei
Citation: Yang, Chun-Jun; Chen, Heng-Li; Cheng, Hsi-Tsung; Chiu, Chui-Lin; Cheng, Jya-Wei. "Structural Characterization and DNA Binding Sequence Selection of the CsrA Protein from Helicobacter pylori" .
Assembly members:
HP1442, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETBlue-2
Entity Sequences (FASTA):
HP1442: MALILSRKVNEGIVIDDNIH
IKVISIDRGSVRLGFEAPES
TLILRAELKEAIVSENQKAS
VCVDESLLENIKKVIKPLEH
HHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 227 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HP1442, chain A | 1 |
| 2 | HP1442, chain B | 1 |
Entities:
Entity 1, HP1442, chain A 85 residues - Formula weight is not available
Residues 78-85 represent a non-native affinity tag and residue 2 is an artefact from cloning.
| 1 | MET | ALA | LEU | ILE | LEU | SER | ARG | LYS | VAL | ASN | ||||
| 2 | GLU | GLY | ILE | VAL | ILE | ASP | ASP | ASN | ILE | HIS | ||||
| 3 | ILE | LYS | VAL | ILE | SER | ILE | ASP | ARG | GLY | SER | ||||
| 4 | VAL | ARG | LEU | GLY | PHE | GLU | ALA | PRO | GLU | SER | ||||
| 5 | THR | LEU | ILE | LEU | ARG | ALA | GLU | LEU | LYS | GLU | ||||
| 6 | ALA | ILE | VAL | SER | GLU | ASN | GLN | LYS | ALA | SER | ||||
| 7 | VAL | CYS | VAL | ASP | GLU | SER | LEU | LEU | GLU | ASN | ||||
| 8 | ILE | LYS | LYS | VAL | ILE | LYS | PRO | LEU | GLU | HIS | ||||
| 9 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: HP1442, [U-13C; U-15N], 1 mM; potassium phosphate 30 mM; sodium chloride 50 mM; EDTA 0.1 mM; DTT 5 mM; sodium azide 10 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis
xwinnmr, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| GB | NP_208233 AAD08480 ABF85435 ACD48869 ACJ08568 ACX98483 |
| UNP | O25983 |
| DBJ | BAJ57406 BAJ58911 BAJ60431 BAM97263 BAM98719 |
| EMBL | CAX30116 CBI65651 |
| REF | NP_208233 WP_000906438 WP_000906439 WP_000906441 WP_000906442 |
| SP | B2UVI7 B6JNU0 O25983 |
| AlphaFold | O25983 O25983 B2UVI7 B6JNU0 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts