BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17183

Title: Solid-state 31P NMR Spectroscopy of Microcrystals of the Ras Protein and its Effector Loop Mutants: Comparison Between Crystalline and Solution State   PubMed: 15342254

Deposition date: 2010-09-10 Original release date: 2010-11-10

Authors: Iuga, Adriana; Spoerner, Michael; Kalbitzer, Hans; Brunner, Eike

Citation: Iuga, Adriana; Spoerner, Michael; Kalbitzer, Hans; Brunner, Eike. "Solid-state 31P NMR Spectroscopy of Microcrystals of the Ras Protein and its Effector Loop Mutants: Comparison Between Crystalline and Solution State"  J. Mol. Biol. 342, 1033-1040 (2004).

Assembly members:
Ras, polymer, 166 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.
GNP, non-polymer, 522.196 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ns

Entity Sequences (FASTA):
Ras: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHQYREQI KRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIP YIETSAKTRQGVEDAFYTLV REIRQH

Data sets:
Data typeCount
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ras1
2MG2
3GppNHp3

Entities:

Entity 1, Ras 166 residues - Formula weight is not available

1   METTHRGLUTYRLYSLEUVALVALVALGLY
2   ALAGLYGLYVALGLYLYSSERALALEUTHR
3   ILEGLNLEUILEGLNASNHISPHEVALASP
4   GLUTYRASPPROTHRILEGLUASPSERTYR
5   ARGLYSGLNVALVALILEASPGLYGLUTHR
6   CYSLEULEUASPILELEUASPTHRALAGLY
7   GLNGLUGLUTYRSERALAMETARGASPGLN
8   TYRMETARGTHRGLYGLUGLYPHELEUCYS
9   VALPHEALAILEASNASNTHRLYSSERPHE
10   GLUASPILEHISGLNTYRARGGLUGLNILE
11   LYSARGVALLYSASPSERASPASPVALPRO
12   METVALLEUVALGLYASNLYSCYSASPLEU
13   ALAALAARGTHRVALGLUSERARGGLNALA
14   GLNASPLEUALAARGSERTYRGLYILEPRO
15   TYRILEGLUTHRSERALALYSTHRARGGLN
16   GLYVALGLUASPALAPHETYRTHRLEUVAL
17   ARGGLUILEARGGLNHIS

Entity 2, MG - Mg - 24.305 Da.

1   MG

Entity 3, GppNHp - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Samples:

sample_1: Ras 1.5 mM; MG 1.5 mM; GppNHp 1.5 mM; Tris-HCl 50 mM; magnesium chloride 10 mM; dithioerythritiol 5 mM; polyethylene glycol 400 56%

sample_conditions_1: pH: 7.6; pressure: ambient atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
31P NMRsample_1anisotropicsample_conditions_1

Software:

SIMPSON, Bak, M., Rasmussen, J. T. & Nielsen, N. C. (2000). - numerical spectra simulations

NMR spectrometers:

  • Bruker Avance 300 MHz

Related Database Links:

BMRB 10051 17610 17678 18461 18479 18629 25730
PDB
DBJ BAB61869 BAB61870 BAB88314 BAB88315 BAB88316
EMBL CAA25322 CAA25624 CAA27258 CAA35240 CAA90306
GB AAA36554 AAA42009 AAA46568 AAA46569 AAA46570
PIR A43816 TVMVNS
PRF 0904302A 1604384A
REF NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913
SP P01112 P01113 P01114 P01115 P08642
TPG DAA13500
AlphaFold P01115 P01113 P01112 P01114 P08642