Biological Magnetic Resonance Data Bank

A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17511

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations.   PubMed: 17469817

Deposition date: 2011-03-06 Original release date: 2011-04-04

Authors: Nikolaev, Yaroslav; Pervushin, Konstantin

Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "NMR spin state exchange spectroscopy reveals equilibrium of two distinct conformations of leucine zipper GCN4 in solution."  J. Am. Chem. Soc. 129, 6461-6469 (2007).

Assembly members:
LZ-GCN4, polymer, 36 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts69
15N chemical shifts104
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Entity Assembly IDEntity NameEntity ID
1LZ-GCN4 dimer, chain 11
2LZ-GCN4 dimer, chain 21
3LZ-GCN4 monomer1


Entity 1, LZ-GCN4 dimer, chain 1 36 residues - Formula weight is not available

First Glycine is a non-native residue from the expression system.



LZ-GCN4_NC: LZ-GCN4, [U-13C; U-15N], 0.3 mM; potassium chloride 80 mM; sodium acetate 40 mM

pH_3.2_310K: pH: 3.2; pressure: 1 atm; temperature: 310 K

pH_7.1_310K: pH: 7.1; temperature: 310 K


NameSampleSample stateSample conditions
3D HNCACBLZ-GCN4_NCisotropicpH_3.2_310K
2D 1H-15N HSQCLZ-GCN4_NCisotropicpH_7.1_310K
3D 1H-15N NOESYLZ-GCN4_NCisotropicpH_7.1_310K


CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker AMX 900 MHz

Related Database Links:

BMRB 1396 1397 1398 1399 1451 1452 1453 1454 15316 2062 371
DBJ GAA22815
EMBL CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB AAA34640 AAB64486 AAK07887 AAL09032 AHY75543
REF NP_010907
SP P03069
TPG DAA07643
AlphaFold P03069

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts