BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18615

Title: 1H, 13C and 15N chemical shift assignments of human parvulin 17   PubMed: 23179059

Deposition date: 2012-07-25 Original release date: 2015-05-05

Authors: Lin, Yi-Jan; Schmidt, Andreas; Burgardt, Noelia; Thiele, Alexandra; Weiwad, Matthias; Luecke, Christian

Citation: Lin, Yi-Jan; Schmidt, Andreas; Burgardt, Noelia; Thiele, Alexandra; Weiwad, Matthias; Luecke, Christian. "1H, 13C and 15N resonance assignments of human parvulin 17"  Biomol. NMR Assign. 7, 325-329 (2013).

Assembly members:
Par17, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-T4-1

Entity Sequences (FASTA):
Par17: GSMPMAGLLKGLVRQLEQFR VQQQASKMPPKGKSGSGKAG KGGAASGSDSADKKAQGPKG GGNAVKVRHILCEKHGKIME AMEKLKSGMRFNEVAAQYSE DKARQGGDLGWMTRGSMVGP FQEAAFALPVSGMDKPVFTD PPVKTKFGYHIIMVEGRK

Data sets:
Data typeCount
13C chemical shifts662
15N chemical shifts153
1H chemical shifts773

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Par171

Entities:

Entity 1, Par17 158 residues - Formula weight is not available

The first two residues Gly(-1) and Ser0 are the residual of a non-native thrombin cleavage site

1   GLYSERMETPROMETALAGLYLEULEULYS
2   GLYLEUVALARGGLNLEUGLUGLNPHEARG
3   VALGLNGLNGLNALASERLYSMETPROPRO
4   LYSGLYLYSSERGLYSERGLYLYSALAGLY
5   LYSGLYGLYALAALASERGLYSERASPSER
6   ALAASPLYSLYSALAGLNGLYPROLYSGLY
7   GLYGLYASNALAVALLYSVALARGHISILE
8   LEUCYSGLULYSHISGLYLYSILEMETGLU
9   ALAMETGLULYSLEULYSSERGLYMETARG
10   PHEASNGLUVALALAALAGLNTYRSERGLU
11   ASPLYSALAARGGLNGLYGLYASPLEUGLY
12   TRPMETTHRARGGLYSERMETVALGLYPRO
13   PHEGLNGLUALAALAPHEALALEUPROVAL
14   SERGLYMETASPLYSPROVALPHETHRASP
15   PROPROVALLYSTHRLYSPHEGLYTYRHIS
16   ILEILEMETVALGLUGLYARGLYS

Samples:

sample_1: Par17 0.9 mM; MES 10 mM; potassium chloride 50 mM; calcium chloride 6 mM

sample_2: Par17, [U-98% 13C; U-98% 15N], 0.9 mM; MES 10 mM; potassium chloride 50 mM; calcium chloride 6 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
HN(CA)COsample_2isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY v3, Goddard - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

GB AAH93700 AAD27893 AAH05234 AAH70288 AAH93700 AAI04654
PDB
DBJ BAA82320 BAB26863 BAG54532
REF NP_001164218 NP_006214 NP_081457 XP_001119408 XP_002720151
SP Q9CWW6 Q9Y237
AlphaFold Q9CWW6 Q9Y237

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts