BMRB Entry 20108

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20108

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Title:
Conformational Flexibility of SecA IRA1 Loop Region
Deposition date:
2009-11-27
Original release date:
2011-06-02
Authors:
Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Ragone, Raffaele; Benedetti, Ettore; Rossi, Filomena
Citation:

Citation: Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Benedetti, Ettore; Rossi, Filomena; Ragone, Raffaele. "Structural determinants of protein translocation in bacteria: conformational flexibility of SecA IRA1 loop region."  J. Pept. Sci. 17, 263-269 (2011).
PubMed: 21337475

Assembly members:

Assembly members:
SecA[788-804], polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SecA[788-804]: GIHLRGYAQKDPKQEYK

Data sets:
Data typeCount
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SecA[788-804]1

Entities:

Entity 1, SecA[788-804] 17 residues - Formula weight is not available

1   GLYILEHISLEUARGGLYTYRALAGLNLYS
2   ASPPROLYSGLNGLUTYRLYS

Samples:

sample_1: SecA[788-804] 1 mM; TFEd2-OH, [U-100% 2H], 50 % v/v; H2O 50 % v/v

sample_conditions_1: pH: 5.1; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB12918 BAB33525 BAB96666 BAC24346 BAE73732
EMBL CAA38875 CAD01293 CAD16541 CAD83669 CAE16018
GB AAA24619 AAC22566 AAC73209 AAD22450 AAG54402
PIR AE0519 F84953
REF NP_240032 NP_308129 NP_414640 NP_439069 NP_454748
SP A1A7E3 A1JJK2 A1SU27 A3MYW1 A4TQ74
AlphaFold A1A7E3 A1JJK2 A1SU27 A3MYW1 A4TQ74