BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25078

Title: CSD1-UNR bound to msl2 mRNA and Sex-lethal   PubMed: 25209665

Deposition date: 2014-07-04 Original release date: 2014-11-07

Authors: Hennig, Janosch; Wang, Iren; Sattler, Michael

Citation: Hennig, Janosch; Militti, Cristina; Popowicz, Grzegorz; Wang, Iren; Sonntag, Miriam; Geerlof, Arie; Gabel, Frank; Gebauer, Fatima; Sattler, Michael. "Structural basis for the assembly of the Sxl-Unr translation regulatory complex"  Nature 515, 287-290 (2014).

Assembly members:
UNR, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTRX-CSD

Entity Sequences (FASTA):
UNR: GAMATRETGIIEKLLHSYGF IQCCERQARLFFHFSQFSGN IDHLKIGDPVEFEMTYDRRT GKPIASQVSKIA

Data sets:
Data typeCount
1H chemical shifts64
13C chemical shifts91
15N chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CSD11

Entities:

Entity 1, CSD1 72 residues - Formula weight is not available

1   GLYALAMETALATHRARGGLUTHRGLYILE
2   ILEGLULYSLEULEUHISSERTYRGLYPHE
3   ILEGLNCYSCYSGLUARGGLNALAARGLEU
4   PHEPHEHISPHESERGLNPHESERGLYASN
5   ILEASPHISLEULYSILEGLYASPPROVAL
6   GLUPHEGLUMETTHRTYRASPARGARGTHR
7   GLYLYSPROILEALASERGLNVALSERLYS
8   ILEALA

Samples:

sample_1: UNR, [U-13C; U-15N; U-2H], 0.2 – 0.5 mM; SXL-RRM1-RRM20.2 – 0.5 mM; 18-mer RNA0.2 – 0.5 mM; DTT 10 mM; potassium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%; sodium chloride 90%; sodium azide 10%

sample_conditions_1: temperature: 310 K; pH: 6.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D TROSY-HNCAsample_1isotropicsample_conditions_1

Software:

CARA, Fred Damberger - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

BMRB 25059 25060
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts