BMRB Entry 26698
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26698
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for rNedd4 WW3 Domain PubMed: 26841867
Deposition date: 2015-10-27 Original release date: 2016-02-22
Authors: Spagnol, Gaelle; Kieken, Fabien; Sorgen, Paul
Citation: Spagnol, Gaelle; Kieken, Fabien; Kopanic, Jennifer; Li, Hanjun; Zach, Sydney; Stauch, Kelly; Grosely, Rosslyn; Sorgen, Paul. "Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus" J. Biol. Chem. 291, 7637-7650 (2016).
Assembly members:
Nedd4_WW3, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-KT
Entity Sequences (FASTA):
Nedd4_WW3: GSLGPLPPGWEERTHTDGRV
FFINHNIKKTQWEDPRMQN
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 133 |
15N chemical shifts | 38 |
1H chemical shifts | 240 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | rNedd4 WW3 Domain | 1 |
Entities:
Entity 1, rNedd4 WW3 Domain 39 residues - Formula weight is not available
1 | GLY | SER | LEU | GLY | PRO | LEU | PRO | PRO | GLY | TRP | ||||
2 | GLU | GLU | ARG | THR | HIS | THR | ASP | GLY | ARG | VAL | ||||
3 | PHE | PHE | ILE | ASN | HIS | ASN | ILE | LYS | LYS | THR | ||||
4 | GLN | TRP | GLU | ASP | PRO | ARG | MET | GLN | ASN |
Samples:
sample_1: Nedd4 WW3, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 1.8 mM; DTT 1 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 156 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, F. Delaglio, S. Grzesiek, G. W. Vuister, G. Zhu, J. Pfeifer and A. Bax, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking, processing
NMRPipe, F. Delaglio, S. Grzesiek, G. W. Vuister, G. Zhu, J. Pfeifer and A. Bax, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking, processing
NMRDraw, F. Delaglio, S. Grzesiek, G. W. Vuister, G. Zhu, J. Pfeifer and A. Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts