BMRB Entry 27124
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27124
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Title: hnRNPA2 D290V low complexity domain 190-341 backbone and Cbeta chemical shifts PubMed: 29358076
Deposition date: 2017-06-05 Original release date: 2018-01-11
Authors: Ryan, Veronica; Fawzi, Nicolas; Silva, Rute; Burke, Kathleen
Citation: Ryan, Veronica; Dignon, Gregory; Zerze, Gul; Chabata, Charlene; Silva, Rute; Conicella, Alexander; Amaya, Joshua; Burke, Kathleen; Mittal, Jeetain; Fawzi, Nicolas. "Disease mutation, protein interactions, and posttranslational modifications modulate hnRNPA2 liquid-liquid phase separation" Mol. Cell 69, 465-479 (2018).
Assembly members:
hnRNPA2_LC_D290V, polymer, 155 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: hnRNPA2_LC_D290V_pJ411
Entity Sequences (FASTA):
hnRNPA2_LC_D290V: GHMGRGGNFGFGDSRGGGGN
FGPGPGSNFRGGSDGYGSGR
GFGDGYNGYGGGPGGGNFGG
SPGYGGGRGGYGGGGPGYGN
QGGGYGGGYDNYGGGNYGSG
NYNVFGNYNQQPSNYGPMKS
GNFGGSRNMGGPYGGGNYGP
GGSGGSGGYGGRSRY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 359 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hnRNPA2 LC D290V | 1 |
Entities:
Entity 1, hnRNPA2 LC D290V 155 residues - Formula weight is not available
Resides 1-3 are cloning and cleavage remnants, residue 4 is residue 190 in human hnRNPA2 LC
| 1 | GLY | HIS | MET | GLY | ARG | GLY | GLY | ASN | PHE | GLY | ||||
| 2 | PHE | GLY | ASP | SER | ARG | GLY | GLY | GLY | GLY | ASN | ||||
| 3 | PHE | GLY | PRO | GLY | PRO | GLY | SER | ASN | PHE | ARG | ||||
| 4 | GLY | GLY | SER | ASP | GLY | TYR | GLY | SER | GLY | ARG | ||||
| 5 | GLY | PHE | GLY | ASP | GLY | TYR | ASN | GLY | TYR | GLY | ||||
| 6 | GLY | GLY | PRO | GLY | GLY | GLY | ASN | PHE | GLY | GLY | ||||
| 7 | SER | PRO | GLY | TYR | GLY | GLY | GLY | ARG | GLY | GLY | ||||
| 8 | TYR | GLY | GLY | GLY | GLY | PRO | GLY | TYR | GLY | ASN | ||||
| 9 | GLN | GLY | GLY | GLY | TYR | GLY | GLY | GLY | TYR | ASP | ||||
| 10 | ASN | TYR | GLY | GLY | GLY | ASN | TYR | GLY | SER | GLY | ||||
| 11 | ASN | TYR | ASN | VAL | PHE | GLY | ASN | TYR | ASN | GLN | ||||
| 12 | GLN | PRO | SER | ASN | TYR | GLY | PRO | MET | LYS | SER | ||||
| 13 | GLY | ASN | PHE | GLY | GLY | SER | ARG | ASN | MET | GLY | ||||
| 14 | GLY | PRO | TYR | GLY | GLY | GLY | ASN | TYR | GLY | PRO | ||||
| 15 | GLY | GLY | SER | GLY | GLY | SER | GLY | GLY | TYR | GLY | ||||
| 16 | GLY | ARG | SER | ARG | TYR |
Samples:
sample_1: hnRNPA2 LC D290V, [U-99% 13C; U-99% 15N], 100 uM; D2O, [U-2H], 10%; MES 20 mM; Bis Tris 1 ± 1 mM; H2O 90%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CAN)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Wuthrich - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts