BMRB Entry 27352
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27352
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Title: Backbone 1H, 13C, and 15N assignments of the extracellular region of human Fc receptor IIIb PubMed: 29453714
Deposition date: 2018-01-04 Original release date: 2018-02-20
Authors: Yogo, Rina; Yanaka, Saeko; Kato, Koichi
Citation: Yogo, Rina; Yanaka, Saeko; Kato, Koichi. "Backbone 1H, 13C, and 15N assignments of the extracellular region of human Fcgammaa receptor IIIb" Biomol. NMR Assignments 12, 201-204 (2018).
Assembly members:
Fc_receptor_IIIb, polymer, 187 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28b
Entity Sequences (FASTA):
Fc_receptor_IIIb: MARTEDLPKAVVFLEPQWYS
VLEKDSVTLKCQGAYSPEDN
STQWFHNESLISSQASSYFI
DAATVNDSGEYRCQTNLSTL
SDPVQLEVHIGWLLLQAPRW
VFKEEDPIHLRCHSWKNTAL
HKVTYLQNGKDRKYFHHNSD
FHIPKATLKDSGSYFCRGLV
GSKNVSSETVNITITQAAAL
EHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 501 |
| 15N chemical shifts | 167 |
| 1H chemical shifts | 167 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fc receptor IIIb | 1 |
Entities:
Entity 1, Fc receptor IIIb 187 residues - Formula weight is not available
| 1 | MET | ALA | ARG | THR | GLU | ASP | LEU | PRO | LYS | ALA | ||||
| 2 | VAL | VAL | PHE | LEU | GLU | PRO | GLN | TRP | TYR | SER | ||||
| 3 | VAL | LEU | GLU | LYS | ASP | SER | VAL | THR | LEU | LYS | ||||
| 4 | CYS | GLN | GLY | ALA | TYR | SER | PRO | GLU | ASP | ASN | ||||
| 5 | SER | THR | GLN | TRP | PHE | HIS | ASN | GLU | SER | LEU | ||||
| 6 | ILE | SER | SER | GLN | ALA | SER | SER | TYR | PHE | ILE | ||||
| 7 | ASP | ALA | ALA | THR | VAL | ASN | ASP | SER | GLY | GLU | ||||
| 8 | TYR | ARG | CYS | GLN | THR | ASN | LEU | SER | THR | LEU | ||||
| 9 | SER | ASP | PRO | VAL | GLN | LEU | GLU | VAL | HIS | ILE | ||||
| 10 | GLY | TRP | LEU | LEU | LEU | GLN | ALA | PRO | ARG | TRP | ||||
| 11 | VAL | PHE | LYS | GLU | GLU | ASP | PRO | ILE | HIS | LEU | ||||
| 12 | ARG | CYS | HIS | SER | TRP | LYS | ASN | THR | ALA | LEU | ||||
| 13 | HIS | LYS | VAL | THR | TYR | LEU | GLN | ASN | GLY | LYS | ||||
| 14 | ASP | ARG | LYS | TYR | PHE | HIS | HIS | ASN | SER | ASP | ||||
| 15 | PHE | HIS | ILE | PRO | LYS | ALA | THR | LEU | LYS | ASP | ||||
| 16 | SER | GLY | SER | TYR | PHE | CYS | ARG | GLY | LEU | VAL | ||||
| 17 | GLY | SER | LYS | ASN | VAL | SER | SER | GLU | THR | VAL | ||||
| 18 | ASN | ILE | THR | ILE | THR | GLN | ALA | ALA | ALA | LEU | ||||
| 19 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Fc receptor IIIb, [U-100% 13C; U-100% 15N] partially deuterated, 0.2 mM; sodium chloride 50 mM; sodium phosphate 5 mM
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N trHSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D trCBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D trHNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D trHN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D trHNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D trHNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D trHCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D trHNCANNH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts