BMRB Entry 27625
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27625
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Title: 1H, 13C, 15N assignment of the CD44 cytoplasmatic tail (669-742) PubMed: 30474821
Deposition date: 2018-09-25 Original release date: 2019-01-09
Authors: Fruehbauer, Benjamin; Mateos, Borja; Konrat, Robert
Citation: Fruehbauer, Benjamin; Mateos, Borja; Konrat, Robert. "1H, 15N, 13C resonance assignment of the human CD44 cytoplasmic tail (669-742)" Biomol. NMR Assignments 13, 109-113 (2019).
Assembly members:
CD44ctail, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11
Entity Sequences (FASTA):
CD44ctail: GAMGAVNSRRRCGQKKKLVI
NSGNGAVEDRKPSGLNGEAS
KSQEMVHLVNKESSETPDQF
MTADETRNLQNVDMKIGV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 218 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD44ctail | 1 |
Entities:
Entity 1, CD44ctail 78 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | ALA | VAL | ASN | SER | ARG | ARG | ||||
| 2 | ARG | CYS | GLY | GLN | LYS | LYS | LYS | LEU | VAL | ILE | ||||
| 3 | ASN | SER | GLY | ASN | GLY | ALA | VAL | GLU | ASP | ARG | ||||
| 4 | LYS | PRO | SER | GLY | LEU | ASN | GLY | GLU | ALA | SER | ||||
| 5 | LYS | SER | GLN | GLU | MET | VAL | HIS | LEU | VAL | ASN | ||||
| 6 | LYS | GLU | SER | SER | GLU | THR | PRO | ASP | GLN | PHE | ||||
| 7 | MET | THR | ALA | ASP | GLU | THR | ARG | ASN | LEU | GLN | ||||
| 8 | ASN | VAL | ASP | MET | LYS | ILE | GLY | VAL |
Samples:
sample_1: CD44ctail, [U-100% 13C; U-100% 15N], 4.5 mM; D2O, [U-2H], 10%; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR, Bruker Biospin, CCPN - chemical shift assignment, collection, processing
NMR spectrometers:
- Bruker Neo 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts