BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30344

Title: PawL-Derived Peptide PLP-10 (cis conformer)   PubMed: 30417166

Deposition date: 2017-09-11 Original release date: 2018-03-01

Authors: Fisher, M.; Mylne, J.; Howard, M.

Citation: Fisher, Mark; Zhang, Jingjing; Taylor, Nicolas; Howard, Mark; Berkowitz, Oliver; Debowski, Aleksandra; Behsaz, Bahar; Whelan, James; Pevzner, Pavel; Mylne, Joshua. "A family of small, cyclic peptides buried in preproalbumin since the Eocene epoch"  Plant Direct 2, e00042-e00042 (2018).

Assembly members:
GLY-SER-PRO-LEU-PHE-ASP, polymer, 6 residues, 634.679 Da.

Natural source:   Common Name: Zinnia violacea   Taxonomy ID: 34245   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Zinnia Violacea

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
GLY-SER-PRO-LEU-PHE-ASP: GSPLFD

Data typeCount
13C chemical shifts15
1H chemical shifts30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 6 residues - 634.679 Da.

1   GLYSERPROLEUPHEASP

Samples:

sample_1: PLP-10 1.0 ± 0.2 mg/mL

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSY 80sample_1isotropicsample_conditions_1
2D 1H-1H TOCSY 20sample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2, CCPN - data analysis

TOPSPIN v3.5, Bruker Biospin - collection

YASARA v16.7.22, Elmar Krieger - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceIII 600 MHz