BMRB Entry 31199

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31199

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Title:
Solution structure of the translation initiation factor IF-1 from Neisseria gonorrhoeae (NCCP11945). Seattle Structural Genomics Center for Infectious Disease target NegoA.17902.a
Deposition date:
2024-08-27
Original release date:
2024-10-25
Authors:
Buchko, G.
Citation:

Citation: Buchko, G.; van Voorhis, W.; Craig, J.; Myler, P.. "Structural characterization of the translation initiation factor IF-1 from Neisseria gonorrhoeae."  .

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, 9339.878 Da.

Natural source:

Natural source:   Common Name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)   Taxonomy ID: 242231   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria gonorrhoeae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHHHHHHMAKEDTIQMQGE ILETLPNATFKVKLENDHIV LGHISGKMRMHYIRISPGDK VTVELTPYDLTRARIVFRAR

Data typeCount
13C chemical shifts269
15N chemical shifts66
1H chemical shifts406

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 80 residues - 9339.878 Da.

1   METALAHISHISHISHISHISHISMETALA
2   LYSGLUASPTHRILEGLNMETGLNGLYGLU
3   ILELEUGLUTHRLEUPROASNALATHRPHE
4   LYSVALLYSLEUGLUASNASPHISILEVAL
5   LEUGLYHISILESERGLYLYSMETARGMET
6   HISTYRILEARGILESERPROGLYASPLYS
7   VALTHRVALGLULEUTHRPROTYRASPLEU
8   THRARGALAARGILEVALPHEARGALAARG

Samples:

sample_1: sodium chloride 200 ± 4 mM; TRIS 20 ± 1 mM; EDTA 1 ± 0.05 mM; DTT 1 ± 0.05 mM; R20, [U-98% 13C; U-98% 15N], 0.8 ± 0.05 mM

sample_2: sodium chloride 200 ± 4 mM; TRIS 20 ± 1 mM; EDTA 1 ± 0.05 mM; DTT 1 ± 0.05 mM; R20, [U-10% 13C; U-99% 15N], 0.2 ± 0.05 mM

sample_conditions_1: ionic strength: 222 mM; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-15N HSQC Deuterium Exchangesample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v1.2, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS vSOLVE 1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

Poky, Manthey, Tonelli, Clos II, Rahimi, Markley and Lee - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS+, Yang Shen, Frank Delaglio, Gabriel Cornilescu, and Ad Bax - data analysis

NMR spectrometers:

  • Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks