BMRB Entry 34507
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34507
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Title: Solution NMR Structure of APP TMD PubMed: 33232115
Deposition date: 2020-03-30 Original release date: 2020-12-04
Authors: Silber, M.; Muhle-Goll, C.
Citation: Silber, M.; Hitzenberger, M.; Zacharias, M.; Muhle-Goll, C.. "Altered Hinge Conformations in APP Transmembrane Helix Mutants May Affect Enzyme-Substrate Interactions of gamma-secretase" ACS Chem. Neurosci. 11, 4426-4433 (2020).
Assembly members:
entity_1, polymer, 30 residues, 3127.031 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: SNKGAIIGLMVGLVVIATVI
VITLVMLKKK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 98 |
1H chemical shifts | 218 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 30 residues - 3127.031 Da.
1 | SER | ASN | LYS | GLY | ALA | ILE | ILE | GLY | LEU | MET | |
2 | VAL | GLY | LEU | VAL | VAL | ILE | ALA | THR | VAL | ILE | |
3 | VAL | ILE | THR | LEU | VAL | MET | LEU | LYS | LYS | LYS |
Samples:
sample_1: APP G38L 500 uM
sample_conditions_1: ionic strength: 0 Not defined; pH: 7.0; pressure: 1 Pa; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-1H-NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
ARIA v2.3.2, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis v2.4.2, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED. - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker CP TCI 600 MHz