BMRB Entry 4038
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4038
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Citation: Alam, Steven; Volkman, Brian; Markley, John; Satterlee, James. "Detailed NMR Analysis of the Heme-Protein Interactions in Component IV Glycera
dibranchiata Monomeric Hemoglobin-CO" J. Biomol. NMR 11, 119-133 (1998).
Assembly members:
component IV globin of Glycera dibranchiata, polymer, 147 residues, 15021 Da.
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.
CARBON MONOXIDE, non-polymer, 28.010 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d
Entity Sequences (FASTA):
component IV globin of Glycera dibranchiata: GLSAAQRQVVASTWKDIAGS
DNGAGVGKECFTKFLSAHHD
MAAVFGFSGASDPGVADLGA
KVLAQIGVAVSHLGDEGKMV
AEMKAVGVRHKGYGNKHIKA
EYFEPLGASLLSAMEHRIGG
KMNAAAKDAWAAAYADISGA
LISGLQS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 581 |
| 15N chemical shifts | 168 |
| 1H chemical shifts | 975 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GMG4 | 1 |
| 2 | Heme | 2 |
| 3 | CO | 3 |
Entities:
Entity 1, GMG4 147 residues - 15021 Da.
| 1 | GLY | LEU | SER | ALA | ALA | GLN | ARG | GLN | VAL | VAL | ||||
| 2 | ALA | SER | THR | TRP | LYS | ASP | ILE | ALA | GLY | SER | ||||
| 3 | ASP | ASN | GLY | ALA | GLY | VAL | GLY | LYS | GLU | CYS | ||||
| 4 | PHE | THR | LYS | PHE | LEU | SER | ALA | HIS | HIS | ASP | ||||
| 5 | MET | ALA | ALA | VAL | PHE | GLY | PHE | SER | GLY | ALA | ||||
| 6 | SER | ASP | PRO | GLY | VAL | ALA | ASP | LEU | GLY | ALA | ||||
| 7 | LYS | VAL | LEU | ALA | GLN | ILE | GLY | VAL | ALA | VAL | ||||
| 8 | SER | HIS | LEU | GLY | ASP | GLU | GLY | LYS | MET | VAL | ||||
| 9 | ALA | GLU | MET | LYS | ALA | VAL | GLY | VAL | ARG | HIS | ||||
| 10 | LYS | GLY | TYR | GLY | ASN | LYS | HIS | ILE | LYS | ALA | ||||
| 11 | GLU | TYR | PHE | GLU | PRO | LEU | GLY | ALA | SER | LEU | ||||
| 12 | LEU | SER | ALA | MET | GLU | HIS | ARG | ILE | GLY | GLY | ||||
| 13 | LYS | MET | ASN | ALA | ALA | ALA | LYS | ASP | ALA | TRP | ||||
| 14 | ALA | ALA | ALA | TYR | ALA | ASP | ILE | SER | GLY | ALA | ||||
| 15 | LEU | ILE | SER | GLY | LEU | GLN | SER |
Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Entity 3, CO - C O - 28.010 Da.
| 1 | CMO |
Samples:
sample_one: component IV glycera dibranchiata monomeric hemoglobin-CO, [U-15N;13C]-GMG4, 3.25 mM; H2O 10%; D2O 90%; KCL 100 mM; potasium phosphate buffer 100 mM; CO(g) saturated
sample_conditions_one: pH: 5.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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