BMRB Entry 50190

Name: NMR backbone assignment and dynamics of Heimdallarchaeota Profilin from the superphylum Asgard
Published: 2021-07-27

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PDB ID: 6yrr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50190
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR backbone assignment and dynamics of Heimdallarchaeota Profilin from the superphylum Asgard PubMed: 32985518

Deposition date: 2020-02-04 Original release date: 2021-07-27

Authors: Chi, Celestine

Citation: Haq, Syed Razaul; Survery, Sabeen; Hurtig, Fredrik; Lindas, Ann-Christin; Chi, Celestine. "NMR resonance assignment and dynamics of profilin from Heimdallarchaeota" Sci. Rep. 10, 15867-15867 (2020).

Assembly members:
Heimdallarchaeota, polymer, 148 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: 1936272 Superkingdom: Archaea Kingdom: not available Genus/species: not available not available

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSUMO-YHRC

Entity Sequences (FASTA):
Heimdallarchaeota: MKDGFILKKKKIPKNIFRIG NKEMSYSDDLNSLIQSQYVS YVVILDPNGAIYWTNNENWQ VNGSEVLRQWMGSAPSITVA GTKFSSFRNEPGVSFVGRNM AGGGLIILQKAPNGYVFLSW TSHEFLTTSGLPPLNIHAEI AMMAAKFQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	352
15N chemical shifts	130
1H chemical shifts	664

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	full length protein	1

Entities:

Entity 1, full length protein 148 residues - Formula weight is not available

1

MET

LYS

ASP

GLY

PHE

ILE

LEU

LYS

2

LYS

ILE

PRO

LYS

ASN

ILE

PHE

ARG

ILE

GLY

3

ASN

LYS

GLU

MET

SER

TYR

SER

ASP

LEU

4

ASN

SER

LEU

ILE

GLN

SER

GLN

TYR

VAL

SER

5

TYR

VAL

ILE

LEU

ASP

PRO

ASN

GLY

ALA

6

ILE

TYR

TRP

THR

ASN

GLU

ASN

TRP

GLN

7

VAL

ASN

GLY

SER

GLU

VAL

LEU

ARG

GLN

TRP

8

MET

GLY

SER

ALA

PRO

SER

ILE

THR

VAL

ALA

9

GLY

THR

LYS

PHE

SER

PHE

ARG

ASN

GLU

10

PRO

GLY

VAL

SER

PHE

VAL

GLY

ARG

ASN

MET

11

ALA

GLY

LEU

ILE

LEU

GLN

LYS

12

ALA

PRO

ASN

GLY

TYR

VAL

PHE

LEU

SER

TRP

13

THR

SER

HIS

GLU

PHE

LEU

THR

SER

GLY

14

LEU

PRO

LEU

ASN

ILE

HIS

ALA

GLU

ILE

15

ALA

MET

ALA

LYS

PHE

GLN

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker neoAvance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts