BMRB Entry 50199
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50199
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Title: 1H, 15N Resonance Assignments of VAMP2(1-96) in HEK-293T Cells PubMed: 32210233
Deposition date: 2020-02-18 Original release date: 2020-02-19
Authors: Wang, Chuchu; Tu, Jia; Zhang, Shengnan; Li, Dan; Liu, Cong
Citation: Wang, Chuchu; Tu, Jia; Zhang, Shengnan; Cai, Bin; Liu, Zhenying; Hou, Shouqiao; Zhong, Qinglu; Hu, Xiao; Liu, Wenbin; Li, Guohui; Liu, Zhijun; He, Lin; Diao, Jiajie; Zhu, Zheng-Jiang; Li, Dan; Liu, Cong. "Different regions of synaptic vesicle membrane regulate VAMP2 conformation for the SNARE assembly" Nat. Commun. 11, 1531-1531 (2020).
Assembly members:
entity_1, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDC191
Entity Sequences (FASTA):
entity_1: MSATAATVPPAAPAGEGGPP
APPPNLTSNRRLQQTQAQVD
EVVDIMRVNVDKVLERDQKL
SELDDRADALQAGASQFETS
AAKLKRKYWWKNLKMM
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VAMP2(1-96) in HEK-293T Cells | 1 |
Entities:
Entity 1, VAMP2(1-96) in HEK-293T Cells 96 residues - Formula weight is not available
| 1 | MET | SER | ALA | THR | ALA | ALA | THR | VAL | PRO | PRO | ||||
| 2 | ALA | ALA | PRO | ALA | GLY | GLU | GLY | GLY | PRO | PRO | ||||
| 3 | ALA | PRO | PRO | PRO | ASN | LEU | THR | SER | ASN | ARG | ||||
| 4 | ARG | LEU | GLN | GLN | THR | GLN | ALA | GLN | VAL | ASP | ||||
| 5 | GLU | VAL | VAL | ASP | ILE | MET | ARG | VAL | ASN | VAL | ||||
| 6 | ASP | LYS | VAL | LEU | GLU | ARG | ASP | GLN | LYS | LEU | ||||
| 7 | SER | GLU | LEU | ASP | ASP | ARG | ALA | ASP | ALA | LEU | ||||
| 8 | GLN | ALA | GLY | ALA | SER | GLN | PHE | GLU | THR | SER | ||||
| 9 | ALA | ALA | LYS | LEU | LYS | ARG | LYS | TYR | TRP | TRP | ||||
| 10 | LYS | ASN | LEU | LYS | MET | MET |
Samples:
sample_1: VAMP2(1-96) in HEK-293T Cells, [U-100% 15N], 0.08 ± 0.01 mM
sample_conditions_1: ionic strength: 0.158 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v8.9 - processing
SPARKY v3.115 - chemical shift assignment, chemical shift calculation
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts