BMRB Entry 50210
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50210
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Title: EAP34 PubMed: 33423171
Deposition date: 2020-03-12 Original release date: 2021-07-27
Authors: Pal, Indrani; Prakash, Om
Citation: Pal, Indrani; Mishra, Nitin; Herrera, Alvaro; Dubey, Abhinav; Arthanari, Haribabu; Geisbrecht, Brian; Prakash, Om. "1H, 15N, and 13C backbone resonance assignments of the C4b-binding region from the S. aureus extracellular adherence protein" Biomol. NMR Assign. 15, 183-186 (2021).
Assembly members:
entity_1, polymer, 208 residues, Formula weight is not available
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: obtained from a collaborator Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: VPYSINLNGTSTNILSNLSF
SNKPWTNYKNLTSQIKSVLK
HDRGISEQDLKYAKKAYYTV
YFKNGGKRILQLNSKNYTAN
LVHAKDVKRIEITVKTGTKA
KADRYVPYTIAVNGTSTPIL
SKLKISNKQLISYKYLNDKV
KSVLKSERGISDLDLKFAKQ
AKYTVYFKNGKKQVVNLKSD
IFTPNLFSAKDIKKIDIDVK
QYTKSKKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 581 |
| 15N chemical shifts | 199 |
| 1H chemical shifts | 199 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EAP34 | 1 |
Entities:
Entity 1, EAP34 208 residues - Formula weight is not available
| 1 | VAL | PRO | TYR | SER | ILE | ASN | LEU | ASN | GLY | THR | ||||
| 2 | SER | THR | ASN | ILE | LEU | SER | ASN | LEU | SER | PHE | ||||
| 3 | SER | ASN | LYS | PRO | TRP | THR | ASN | TYR | LYS | ASN | ||||
| 4 | LEU | THR | SER | GLN | ILE | LYS | SER | VAL | LEU | LYS | ||||
| 5 | HIS | ASP | ARG | GLY | ILE | SER | GLU | GLN | ASP | LEU | ||||
| 6 | LYS | TYR | ALA | LYS | LYS | ALA | TYR | TYR | THR | VAL | ||||
| 7 | TYR | PHE | LYS | ASN | GLY | GLY | LYS | ARG | ILE | LEU | ||||
| 8 | GLN | LEU | ASN | SER | LYS | ASN | TYR | THR | ALA | ASN | ||||
| 9 | LEU | VAL | HIS | ALA | LYS | ASP | VAL | LYS | ARG | ILE | ||||
| 10 | GLU | ILE | THR | VAL | LYS | THR | GLY | THR | LYS | ALA | ||||
| 11 | LYS | ALA | ASP | ARG | TYR | VAL | PRO | TYR | THR | ILE | ||||
| 12 | ALA | VAL | ASN | GLY | THR | SER | THR | PRO | ILE | LEU | ||||
| 13 | SER | LYS | LEU | LYS | ILE | SER | ASN | LYS | GLN | LEU | ||||
| 14 | ILE | SER | TYR | LYS | TYR | LEU | ASN | ASP | LYS | VAL | ||||
| 15 | LYS | SER | VAL | LEU | LYS | SER | GLU | ARG | GLY | ILE | ||||
| 16 | SER | ASP | LEU | ASP | LEU | LYS | PHE | ALA | LYS | GLN | ||||
| 17 | ALA | LYS | TYR | THR | VAL | TYR | PHE | LYS | ASN | GLY | ||||
| 18 | LYS | LYS | GLN | VAL | VAL | ASN | LEU | LYS | SER | ASP | ||||
| 19 | ILE | PHE | THR | PRO | ASN | LEU | PHE | SER | ALA | LYS | ||||
| 20 | ASP | ILE | LYS | LYS | ILE | ASP | ILE | ASP | VAL | LYS | ||||
| 21 | GLN | TYR | THR | LYS | SER | LYS | LYS | LYS |
Samples:
sample_1: Extracellular Adherence Protein, [U-100% 13C; U-100% 15N], 800 uM
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Varian INOVA 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts