BMRB Entry 50909

Name: Rules for designing protein fold switches and their implications for the folding code
Published: 2023-01-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50909

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rules for designing protein fold switches and their implications for the folding code

Deposition date:

2021-04-22

Original release date:

2023-01-03

Authors:

He, Yanan; Chen, Yingwei; Ruan, Biao; Choi, Eun; Chen, Yihong; Motabar, Dana; Solomon, Tsega; Simmerman, Richard; Kauffman, Thomas; Gallagher, D.Travis; Bryan, Philip; Orban, JOhn

Citation:

Citation: He, Yanan; Chen, Yingwei; Ruan, Biao; Choi, Eun; Chen, Yihong; Motabar, Dana; Solomon, Tsega; Simmerman, Richard; Kauffman, Thomas; Gallagher, D.Travis; Bryan, Philip; Orban, JOhn. "Design and characterization of a protein fold switching network" Nat. Commun. 14, 431-431 (2023).
PubMed: 36702827

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pA-YRGL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TTYKLILNLKFAFGDTNSEA VDAAEAEKKFKQYANDHGVD GEWTYDDATKTFTVTA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	158
15N chemical shifts	50
1H chemical shifts	50

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	B4	1

Entities:

Entity 1, B4 56 residues - Formula weight is not available

1

THR

TYR

LYS

LEU

ILE

LEU

ASN

LEU

LYS

2

PHE

ALA

PHE

GLY

ASP

THR

ASN

SER

GLU

ALA

3

VAL

ASP

ALA

GLU

ALA

GLU

LYS

PHE

4

LYS

GLN

TYR

ALA

ASN

ASP

HIS

GLY

VAL

ASP

5

GLY

GLU

TRP

THR

TYR

ASP

ALA

THR

LYS

6

THR

PHE

THR

VAL

THR

ALA

Samples:

sample_1: B4, [U-13C; U-15N], 0.3 mM; D2O 5 V/V %; potassium phosphate 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3DHNCACB	sample_1	isotropic	sample_conditions_1
3DCBCACONH	sample_1	isotropic	sample_conditions_1
3DHNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.61 - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks