BMRB Entry 50940
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50940
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Title: Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213 PubMed: 34215685
Deposition date: 2021-05-12 Original release date: 2021-06-14
Authors: Hennig, Janosch
Citation: Vaishali, Vaishali; Dimitrova-Paternoga, Lyudmila; Haubrich, Kevin; Sun, Mai; Ephrussi, Anne; Hennig, Janosch. "Validation and classification of RNA binding proteins identified by mRNA interactome capture" RNA 27, 1173-1185 (2021).
Assembly members:
entity_1, polymer, 214 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11d
Entity Sequences (FASTA):
entity_1: SMEHDDSSTSGTGSHTASNT
SLVPASLKRRGHQHHPRFSG
TRRPNVPNVQEILAALYRGD
SKSALSNLRGETQPEEEQPQ
QQTEEVLPPSRSTLSLPLSE
SVTNSLGSNSPTPTDESSVQ
DEGASNPAAALIAEDAAPPA
GTTTSKSKKKKREKGERSEK
SDKSEKSDRKKKSSGKKERS
KRSNPMEQSSDSLATDLSAG
AIDEGIALADDDDN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 318 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 169 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | aTm1 (1-213) | 1 |
Entities:
Entity 1, aTm1 (1-213) 214 residues - Formula weight is not available
| 1 | SER | MET | GLU | HIS | ASP | ASP | SER | SER | THR | SER | ||||
| 2 | GLY | THR | GLY | SER | HIS | THR | ALA | SER | ASN | THR | ||||
| 3 | SER | LEU | VAL | PRO | ALA | SER | LEU | LYS | ARG | ARG | ||||
| 4 | GLY | HIS | GLN | HIS | HIS | PRO | ARG | PHE | SER | GLY | ||||
| 5 | THR | ARG | ARG | PRO | ASN | VAL | PRO | ASN | VAL | GLN | ||||
| 6 | GLU | ILE | LEU | ALA | ALA | LEU | TYR | ARG | GLY | ASP | ||||
| 7 | SER | LYS | SER | ALA | LEU | SER | ASN | LEU | ARG | GLY | ||||
| 8 | GLU | THR | GLN | PRO | GLU | GLU | GLU | GLN | PRO | GLN | ||||
| 9 | GLN | GLN | THR | GLU | GLU | VAL | LEU | PRO | PRO | SER | ||||
| 10 | ARG | SER | THR | LEU | SER | LEU | PRO | LEU | SER | GLU | ||||
| 11 | SER | VAL | THR | ASN | SER | LEU | GLY | SER | ASN | SER | ||||
| 12 | PRO | THR | PRO | THR | ASP | GLU | SER | SER | VAL | GLN | ||||
| 13 | ASP | GLU | GLY | ALA | SER | ASN | PRO | ALA | ALA | ALA | ||||
| 14 | LEU | ILE | ALA | GLU | ASP | ALA | ALA | PRO | PRO | ALA | ||||
| 15 | GLY | THR | THR | THR | SER | LYS | SER | LYS | LYS | LYS | ||||
| 16 | LYS | ARG | GLU | LYS | GLY | GLU | ARG | SER | GLU | LYS | ||||
| 17 | SER | ASP | LYS | SER | GLU | LYS | SER | ASP | ARG | LYS | ||||
| 18 | LYS | LYS | SER | SER | GLY | LYS | LYS | GLU | ARG | SER | ||||
| 19 | LYS | ARG | SER | ASN | PRO | MET | GLU | GLN | SER | SER | ||||
| 20 | ASP | SER | LEU | ALA | THR | ASP | LEU | SER | ALA | GLY | ||||
| 21 | ALA | ILE | ASP | GLU | GLY | ILE | ALA | LEU | ALA | ASP | ||||
| 22 | ASP | ASP | ASP | ASN |
Samples:
sample_1: aTm1 (1-213), [U-100% 15N], 200 uM; PO4 20 mM; NaCl 150 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts