BMRB Entry 51137
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51137
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Title: Assignment of base 15N and 1H chemical shifts for <5_SL5C> PubMed: 35178672
Deposition date: 2021-10-14 Original release date: 2022-02-18
Authors: Schwalbe, Harald; Mertinkus, Klara; Richter, Christian
Citation: Mertinkus, Klara; Grun, J. Tassilo; Altincekic, Nadide; Kaur Bains, Jasleen; Ceylan, Betul; Ferner, Jan-Peter; Frydman, Lucio; Furtig, Boris; Hengesbach, Martin; Hohmann, Katharina; Hymon, Daniel; Kim, Jihyun; Knezic, Bozana; Novakovic, Mihajlo; Oxenfarth, Andreas; Peter, Stephen; Qureshi, Nusrat; Richter, Christian; Scherf, Tali; Schlundt, Andreas; Schnieders, Robbin; Schwalbe, Harald; Stirnal, Elke; Sudakov, Alexey; Vogele, Jennifer; Wacker, Anna; Weigand, Julia; Wirmer-Bartoschek, Julia; Wirtz Martin, Maria; Wohnert, Jens. "1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2" Biomol. NMR Assignments 16, 17-25 (2022).
Assembly members:
entity_1, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GACCGAAAGGUA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 80 |
15N chemical shifts | 4 |
1H chemical shifts | 115 |
31P chemical shifts | 11 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SL5C | 1 |
Entities:
Entity 1, SL5C 12 residues - Formula weight is not available
1 | G | A | C | C | G | A | A | A | G | G | ||||
2 | U | A |
Samples:
sample_1: SL5c, [U-13C; U-15N], 1 mM; potassium phosphate 25 mM; potassium chloride 50 mM
sample_2: SL5c, [U-13C; U-15N], 1 mM; potassium phosphate 25 mM; potassium chloride 50 mM
sample_conditions_1: pH: 6.2; temperature: 283 K
sample_conditions_2: pH: 6.2; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HMQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
2D 1H-31P DINO | sample_2 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
Software:
NMRFAM-SPARKY - chemical shift assignment
TOPSPIN v3.6.3 - processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz MHz
- Bruker AVANCE III 800 MHz MHz
- Bruker AVANCE III 700 MHz MHz
- Bruker AVANCE I 600 MHz MHz
- Bruker AVANCE I 400 MHz MHz