BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 51137

Title: Assignment of base 15N and 1H chemical shifts for <5_SL5C>   PubMed: 35178672

Deposition date: 2021-10-14 Original release date: 2022-02-18

Authors: Schwalbe, Harald; Mertinkus, Klara; Richter, Christian

Citation: Mertinkus, Klara; Grun, J. Tassilo; Altincekic, Nadide; Kaur Bains, Jasleen; Ceylan, Betul; Ferner, Jan-Peter; Frydman, Lucio; Furtig, Boris; Hengesbach, Martin; Hohmann, Katharina; Hymon, Daniel; Kim, Jihyun; Knezic, Bozana; Novakovic, Mihajlo; Oxenfarth, Andreas; Peter, Stephen; Qureshi, Nusrat; Richter, Christian; Scherf, Tali; Schlundt, Andreas; Schnieders, Robbin; Schwalbe, Harald; Stirnal, Elke; Sudakov, Alexey; Vogele, Jennifer; Wacker, Anna; Weigand, Julia; Wirmer-Bartoschek, Julia; Wirtz Martin, Maria; Wohnert, Jens. "1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2"  Biomol. NMR Assignments 16, 17-25 (2022).

Assembly members:
entity_1, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GACCGAAAGGUA

Data sets:
Data typeCount
13C chemical shifts80
15N chemical shifts4
1H chemical shifts115
31P chemical shifts11

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SL5C1

Entities:

Entity 1, SL5C 12 residues - Formula weight is not available

1   GACCGAAAGG
2   UA

Samples:

sample_1: SL5c, [U-13C; U-15N], 1 mM; potassium phosphate 25 mM; potassium chloride 50 mM

sample_2: SL5c, [U-13C; U-15N], 1 mM; potassium phosphate 25 mM; potassium chloride 50 mM

sample_conditions_1: pH: 6.2; temperature: 283 K

sample_conditions_2: pH: 6.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HMQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-31P DINOsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN v3.6.3 - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz MHz
  • Bruker AVANCE III 800 MHz MHz
  • Bruker AVANCE III 700 MHz MHz
  • Bruker AVANCE I 600 MHz MHz
  • Bruker AVANCE I 400 MHz MHz

Related Database Links:

BMRB 50339