BMRB Entry 51865

Name: Assignments for fibrillar Human Islet Amyloid Polypeptide (hIAPP)
Published: 2024-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51865

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments for fibrillar Human Islet Amyloid Polypeptide (hIAPP)

Deposition date:

2023-03-02

Original release date:

2024-10-23

Authors:

Suladze, Saba; Sankar, Riddhiman; Rodriguez Camargo, Diana; Reif, Bernd

Citation:

Citation: Suladze, Saba; Sustay Martinez, Christian; Rodriguez Camargo, Diana; Engler, Jonas; Rodina, Natalia; Sarkar, Riddhiman; Zacharias, Martin; Reif, Bernd. "Structural Insights into Seeding Mechanisms of hIAPP Fibril Formation" J. Am. Chem. Soc. 146, 13783-13796 (2024).
PubMed: 38723619

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, 3908.319 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTXB1EEI

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	101
15N chemical shifts	34
1H chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hIAPP	1

Entities:

Entity 1, hIAPP 37 residues - 3908.319 Da.

1

LYS

CYS

ASN

THR

ALA

THR

CYS

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

HIS

SER

3

ASN

PHE

GLY

ALA

ILE

LEU

SER

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

Samples:

sample_1: recombinant fibrillar hIAPP, [U-100% 15N], 92.5 mM

sample_conditions_1: ionic strength: 0.030 M; pH: 5.3; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 15N-1H "CP-HSQC"	sample_1	isotropic	sample_conditions_1
(H)CANH	sample_1	isotropic	sample_conditions_1
(H)(CO)CA(CO)NH	sample_1	isotropic	sample_conditions_1
(H)(CA)CB(CA)NH	sample_1	isotropic	sample_conditions_1
(H)CONH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz