BMRB Entry 5784
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5784
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Title: 1H assignment of a human variant of beta2-microglobulin where His 31 was replaced by a Tyr PubMed: 14660575
Deposition date: 2003-04-24 Original release date: 2004-02-12
Authors: Pettirossi, Fabio; Corazza, Alessandra; Viglino, Paolo; Verdone, Giuliana; Esposito, Gennaro
Citation: Corazza, Alessandra; Pettirossi, Fabio; Viglino, Paolo; Verdone, Giuliana; Garcia, Julian; Dumy, Pascal; Giorgetti, Sofia; Mangione, Palma; Andreola, Alessia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro. "Properties of some variants of human beta2-microglobulin and amyloidogenesis" J. Biol. Chem. ., .-..
Assembly members:
beta2-microglobulin, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
beta2-microglobulin: MIQRTPKIQVYSRHPAENGK
SNFLNCYVSGFYPSDIEVDL
LKNGERIEKVEHSDLSFSKD
WSFYLLYYTEFTPTEKDEYA
CRVNHVTLSQPKIVKWDRDM
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 541 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H31Ybeta2-microglobulin | 1 |
Entities:
Entity 1, H31Ybeta2-microglobulin 100 residues - Formula weight is not available
| 1 | MET | ILE | GLN | ARG | THR | PRO | LYS | ILE | GLN | VAL | |
| 2 | TYR | SER | ARG | HIS | PRO | ALA | GLU | ASN | GLY | LYS | |
| 3 | SER | ASN | PHE | LEU | ASN | CYS | TYR | VAL | SER | GLY | |
| 4 | PHE | TYR | PRO | SER | ASP | ILE | GLU | VAL | ASP | LEU | |
| 5 | LEU | LYS | ASN | GLY | GLU | ARG | ILE | GLU | LYS | VAL | |
| 6 | GLU | HIS | SER | ASP | LEU | SER | PHE | SER | LYS | ASP | |
| 7 | TRP | SER | PHE | TYR | LEU | LEU | TYR | TYR | THR | GLU | |
| 8 | PHE | THR | PRO | THR | GLU | LYS | ASP | GLU | TYR | ALA | |
| 9 | CYS | ARG | VAL | ASN | HIS | VAL | THR | LEU | SER | GLN | |
| 10 | PRO | LYS | ILE | VAL | LYS | TRP | ASP | ARG | ASP | MET |
Samples:
sample_1: beta2-microglobulin 0.8 mM; sodium phosphate 70 mM; sodium chloride 100 mM; H2O 100%
Ex-cond_1: pH: 6.5 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
Software:
FELIX v2000 -
NMR spectrometers:
- Bruker Avance 500 MHz