BMRB Entry 6172

Name: Solution structure of a cyanobacterial copper metallochaperone, ScAtx1
Published: 2004-06-29

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PDB ID: 1sb6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6172
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a cyanobacterial copper metallochaperone, ScAtx1

Deposition date:

2004-04-02

Original release date:

2004-06-29

Authors:

Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.; Borrelly, G.; Robinson, N.

Citation:

Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.; Borrelly, G.; Robinson, N.. "Solution structures of a cyanobacterial copper metallochaperone: insight into an atypical copper binding motif" J. Biol. Chem. ., .-. (2004).
PubMed: 15075318

Assembly members:

Assembly members:
apoScAtx1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Synechocystis sp. PCC 6803 Taxonomy ID: 1148 Superkingdom: Eubacteria Kingdom: not available Genus/species: Synechocystis Synechocystis sp. PCC 6803

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apoScAtx1: MTIQLTVPTIACEACAEAVT KAVQNEDAQATVQVDLTSKK VTITSALGEEQLRTAIASAG HEVE

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
15N chemical shifts	67
1H chemical shifts	410

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo ScAtx1	1

Entities:

Entity 1, apo ScAtx1 64 residues - Formula weight is not available

1

MET

THR

ILE

GLN

LEU

THR

VAL

PRO

THR

ILE

2

ALA

CYS

GLU

ALA

CYS

ALA

GLU

ALA

VAL

THR

3

LYS

ALA

VAL

GLN

ASN

GLU

ASP

ALA

GLN

ALA

4

THR

VAL

GLN

VAL

ASP

LEU

THR

SER

LYS

5

VAL

THR

ILE

THR

SER

ALA

LEU

GLY

GLU

6

GLN

LEU

ARG

THR

ALA

ILE

ALA

SER

ALA

GLY

7

HIS

GLU

VAL

GLU

Samples:

sample_1: apoScAtx1, [U-15N], 0.7 mM; phosphate 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 7.0 na; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	not available	sample_cond_1
3D 15N-separated NOESY	sample_1	not available	sample_cond_1
2D TOCSY	sample_1	not available	sample_cond_1
HNHA	sample_1	not available	sample_cond_1

Software:

xwinnmr v2.6 - processing

XEASY v1.3 - structure solution

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

Bruker AVANCE 500 MHz

PDB	1SB6 2XMJ 2XMK 2XMM 2XMT 2XMU 2XMV 4A46
DBJ	BAA17240 BAK49413 BAL28412 BAL31581 BAL34750
GB	AGF50929
REF	NP_440560 WP_010871869 YP_005382427 YP_005385596 YP_005408303