BMRB Entry 6500
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6500
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SIP (74-178) PubMed: 15996101
Deposition date: 2005-02-14 Original release date: 2005-08-16
Authors: Bhattacharya, Shibani; Lee, Young-Tae; Michowski, Wojciech; Filipek, Anna; Kuznicki, Jacek; Chazin, Walter
Citation: Bhattacharya, Shibani; Lee, Young-Tae; Michowski, Wojciech; Filipek, Anna; Kuznicki, Jacek; Chazin, Walter. "The Modular Structure of SIP Facilitates Its Role in Stabilizing Multiprotein Assemblies" Biochemistry 44, 9462-9471 (2005).
Assembly members:
SIP (74-178), polymer, 105 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
SIP (74-178): VKISNYGWDQSDKFVKIYIT
LTGVHQVPTENVQVHFTERS
FDLLVKNLNGKNYSMIVNNL
LKPISVESSSKKVKTDTVII
LCRKKAENTRWDYLTQVEKE
CKEKE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 279 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SIP CS-Domain | 1 |
Entities:
Entity 1, SIP CS-Domain 105 residues - Formula weight is not available
| 1 | VAL | LYS | ILE | SER | ASN | TYR | GLY | TRP | ASP | GLN | ||||
| 2 | SER | ASP | LYS | PHE | VAL | LYS | ILE | TYR | ILE | THR | ||||
| 3 | LEU | THR | GLY | VAL | HIS | GLN | VAL | PRO | THR | GLU | ||||
| 4 | ASN | VAL | GLN | VAL | HIS | PHE | THR | GLU | ARG | SER | ||||
| 5 | PHE | ASP | LEU | LEU | VAL | LYS | ASN | LEU | ASN | GLY | ||||
| 6 | LYS | ASN | TYR | SER | MET | ILE | VAL | ASN | ASN | LEU | ||||
| 7 | LEU | LYS | PRO | ILE | SER | VAL | GLU | SER | SER | SER | ||||
| 8 | LYS | LYS | VAL | LYS | THR | ASP | THR | VAL | ILE | ILE | ||||
| 9 | LEU | CYS | ARG | LYS | LYS | ALA | GLU | ASN | THR | ARG | ||||
| 10 | TRP | ASP | TYR | LEU | THR | GLN | VAL | GLU | LYS | GLU | ||||
| 11 | CYS | LYS | GLU | LYS | GLU |
Samples:
Sample_1: SIP (74-178), [U-100% 13C; U-100% 15N], 1.0 mM; NaPi 20 mM; NaCl 50 mM; DTT 5 mM; H2O 90%; D2O 10%
Exp_cond_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C CT-HSQC | not available | not available | not available |
| 3D-HNCO | not available | not available | not available |
| 3D-HNCACB | not available | not available | not available |
| 3D-CBCA(CO)NH | not available | not available | not available |
Software:
FELIX v2000 - analysis, processing
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| GB | AAC16757 AAH25948 AAH79007 EDL39341 EDM09438 |
| REF | NP_001004208 NP_033916 XP_002928087 XP_003130369 XP_003497720 |
| SP | Q6AYK6 Q9CXW3 |
| AlphaFold | Q6AYK6 Q9CXW3 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts