BMRB Entry 6621
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6621
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Title: 1H, 15N, and 13C Resonance Assignments of Human Interleukin-2 PubMed: 16132828
Deposition date: 2005-05-06 Original release date: 2005-09-01
Authors: Fry, David; Emerson, Stephen; Liu, Chao; Palermo, Robert
Citation: Fry, David; Emerson, Stephen; Liu, Chao; Palermo, Robert. "1H, 15N, and 13C Resonance Assignments of Human Interleukin-2" J. Biomol. NMR 32, 258-258 (2005).
Assembly members:
Interleukin-2, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Interleukin-2: APTSSSTKKTQLQLEHLLLD
LQMILNGINNYKNPKLTRML
TFKFYMPKKATELKHLQCLE
EELKPLEEVLNLAQSKNFHL
RPRDLISNINVIVLELKGSE
TTFMCEYADETATIVEFLNR
WITFCQSIISTLT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 445 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 971 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Interleukin-2 | 1 |
Entities:
Entity 1, Interleukin-2 133 residues - Formula weight is not available
| 1 | ALA | PRO | THR | SER | SER | SER | THR | LYS | LYS | THR | ||||
| 2 | GLN | LEU | GLN | LEU | GLU | HIS | LEU | LEU | LEU | ASP | ||||
| 3 | LEU | GLN | MET | ILE | LEU | ASN | GLY | ILE | ASN | ASN | ||||
| 4 | TYR | LYS | ASN | PRO | LYS | LEU | THR | ARG | MET | LEU | ||||
| 5 | THR | PHE | LYS | PHE | TYR | MET | PRO | LYS | LYS | ALA | ||||
| 6 | THR | GLU | LEU | LYS | HIS | LEU | GLN | CYS | LEU | GLU | ||||
| 7 | GLU | GLU | LEU | LYS | PRO | LEU | GLU | GLU | VAL | LEU | ||||
| 8 | ASN | LEU | ALA | GLN | SER | LYS | ASN | PHE | HIS | LEU | ||||
| 9 | ARG | PRO | ARG | ASP | LEU | ILE | SER | ASN | ILE | ASN | ||||
| 10 | VAL | ILE | VAL | LEU | GLU | LEU | LYS | GLY | SER | GLU | ||||
| 11 | THR | THR | PHE | MET | CYS | GLU | TYR | ALA | ASP | GLU | ||||
| 12 | THR | ALA | THR | ILE | VAL | GLU | PHE | LEU | ASN | ARG | ||||
| 13 | TRP | ILE | THR | PHE | CYS | GLN | SER | ILE | ILE | SER | ||||
| 14 | THR | LEU | THR |
Samples:
sample_1: Interleukin-2, [U-13C; U-15N], 0.4 mM; Sodium acetate, [U-99% 2H], 10 mM; Sodium azide 3 mM
conditions_1: pH: 4.6; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N-HSQC | not available | not available | not available |
| 1H-1H-15N-NOESY-HSQC | not available | not available | not available |
| 1H-13C-HMQC | not available | not available | not available |
| HNCOCACB | not available | not available | not available |
| HNCACB | not available | not available | not available |
| 1H-1H-13C-HCCH-TOCSY | not available | not available | not available |
| 1H-1H-13C-NOESY-HMQC | not available | not available | not available |
Software:
FELIX v2000, Accelrys -
NMRView v5.0.3 -
NMR spectrometers:
- Varian Inova 600 MHz MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts