BMRB Entry 7132
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7132
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Title: 1H, 13C and 15N Chemical Shift Assignments for the CheA P1 domain from Thermotoga maritima PubMed: 16878985
Deposition date: 2006-05-25 Original release date: 2006-10-30
Authors: Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick
Citation: Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick. "Chemical-shift-perturbation mapping of the phosphotransfer and catalytic domain interaction in the histidine autokinase CheA from Thermotoga maritima." Biochemistry 45, 9509-9517 (2006).
Assembly members:
P1, polymer, 131 residues, 14690 Da.
Natural source: Common Name: Thermotoga maritima Taxonomy ID: 243274 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermotoga maritima
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
P1: MMEEYLGVFVDETKEYLQNL
NDTLLELEKNPEDMELINEA
FRALHTLKGMAGTMGFSSMA
KLCHTLENILDKARNSEIKI
TSDLLDKIFAGVDMITRMVD
KIVSEGSDDIGENIDVFSDT
IKSFASSGKEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 276 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 358 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CheA P1 | 1 |
Entities:
Entity 1, CheA P1 131 residues - 14690 Da.
| 1 | MET | MET | GLU | GLU | TYR | LEU | GLY | VAL | PHE | VAL | ||||
| 2 | ASP | GLU | THR | LYS | GLU | TYR | LEU | GLN | ASN | LEU | ||||
| 3 | ASN | ASP | THR | LEU | LEU | GLU | LEU | GLU | LYS | ASN | ||||
| 4 | PRO | GLU | ASP | MET | GLU | LEU | ILE | ASN | GLU | ALA | ||||
| 5 | PHE | ARG | ALA | LEU | HIS | THR | LEU | LYS | GLY | MET | ||||
| 6 | ALA | GLY | THR | MET | GLY | PHE | SER | SER | MET | ALA | ||||
| 7 | LYS | LEU | CYS | HIS | THR | LEU | GLU | ASN | ILE | LEU | ||||
| 8 | ASP | LYS | ALA | ARG | ASN | SER | GLU | ILE | LYS | ILE | ||||
| 9 | THR | SER | ASP | LEU | LEU | ASP | LYS | ILE | PHE | ALA | ||||
| 10 | GLY | VAL | ASP | MET | ILE | THR | ARG | MET | VAL | ASP | ||||
| 11 | LYS | ILE | VAL | SER | GLU | GLY | SER | ASP | ASP | ILE | ||||
| 12 | GLY | GLU | ASN | ILE | ASP | VAL | PHE | SER | ASP | THR | ||||
| 13 | ILE | LYS | SER | PHE | ALA | SER | SER | GLY | LYS | GLU | ||||
| 14 | LYS |
Samples:
sample_1: P1, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM; Na2HPO4 50 mM; NaCl 50 mM; sodium azide 0.04%
conditions_1: ionic strength: 100 mM; pH: 7.40; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NNH | sample_1 | not available | conditions_1 |
| ccc-tocsy | sample_1 | not available | conditions_1 |
| hcch-tocsy | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 17651 |
| PDB | |
| GB | AAA96387 AAD35784 ABQ46257 ACB08586 ADA66595 |
| REF | NP_228511 WP_004081040 WP_008192691 WP_011942905 WP_012310403 |
| SP | Q56310 |
| AlphaFold | Q56310 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts