BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7214

Title: Backbone 1H Chemical Shift Assignments for J3   PubMed: 17060469

Deposition date: 2006-07-03 Original release date: 2007-01-09

Authors: Sabo, Jennifer; Keizer, David; Feng, Zhi-Ping; Casey, Joanne; Parisi, Kathy; Coley, Andrew; Foley, Michael; Norton, Raymond

Citation: Sabo, Jennifer; Keizer, David; Feng, Zhi-Ping; Casey, Joanne; Parisi, Kathy; Coley, Andrew; Foley, Michael; Norton, Raymond. "Mimotopes of Apical Membrane Antigen 1: Structures of Phage-Derived Peptides Recognized by the Inhibitory Monoclonal Antibody 4G2dc1 and Design of a More Active Analogue"  Infect. Immun. 75, 61-73 (2007).

Assembly members:
J3, polymer, 21 residues, Formula weight is not available

Natural source:   Common Name: Enterobacteria phage M13   Taxonomy ID: 10870   Superkingdom: Viruses   Kingdom: not available   Genus/species: Inovirus Inovirus Enterobacteria phage

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
J3: FGGVSDEDVPSFHFKPPSSR G

Data sets:
Data typeCount
1H chemical shifts110
coupling constants11

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1J31

Entities:

Entity 1, J3 21 residues - Formula weight is not available

1   PHEGLYGLYVALSERASPGLUASPVALPRO
2   SERPHEHISPHELYSPROPROSERSERARG
3   GLY

Samples:

sample_1: J3 1 mM; sodium acetate 10 mM

conditions_1: pH: 4.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1not availableconditions_1
2D 1H-1H DQF-COSYsample_1not availableconditions_1
2D 1H-1H NOESYsample_1not availableconditions_1

Software:

xwinnmr v3.5, Bruker Biospin -

XEASY v1.3.13 -

CYANA v1.0.6 -

X-PLOR NIH v2.9.3 -

NMR spectrometers:

  • Bruker Biospin AMX 500 MHz
  • Bruker Biospin DRX 600 MHz