BMRB Entry 7240
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7240
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Campos-Olivas, Ramon; Sanchez, Ricardo; Torres, Daniel; Blanco, Francisco. "Backbone Assignment of the 98kDa homotrimeric yeast PCNA ring" J. Biomol. NMR 38, 167-167 (2006).
PubMed: 17036161
Assembly members:
scPCNA monomer, polymer, 264 residues, 32635 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
scPCNA monomer: GPHMASMLEAKFEEASLFKR
IIDGFKDCVQLVNFQCKEDG
IIAQAVDDSRVLLVSLEIGV
EAFQEYRCDHPVTLGMDLTS
LSKILRCGNNTDTLTLIADN
TPDSIILLFEDTKKDRIAEY
SLKLMDIDADFLKIEELQYD
STLSLPSSEFSKIVRDLSQL
SDSINIMITKETIKFVADGD
IGSGSVIIKPFVDMEHPETS
IKLEMDQPVDLTFGAKYLLD
IIKGSSLSDRVGIRLSSEAP
ALFQFDLKSGFLQFFLAPKF
NDEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 768 |
| 15N chemical shifts | 250 |
| 1H chemical shifts | 251 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | scPCNA monomer 1 | 1 |
| 2 | scPCNA monomer 2 | 1 |
| 3 | scPCNA monomer 3 | 1 |
Entities:
Entity 1, scPCNA monomer 1 264 residues - 32635 Da.
| 1 | GLY | PRO | HIS | MET | ALA | SER | MET | LEU | GLU | ALA | ||||
| 2 | LYS | PHE | GLU | GLU | ALA | SER | LEU | PHE | LYS | ARG | ||||
| 3 | ILE | ILE | ASP | GLY | PHE | LYS | ASP | CYS | VAL | GLN | ||||
| 4 | LEU | VAL | ASN | PHE | GLN | CYS | LYS | GLU | ASP | GLY | ||||
| 5 | ILE | ILE | ALA | GLN | ALA | VAL | ASP | ASP | SER | ARG | ||||
| 6 | VAL | LEU | LEU | VAL | SER | LEU | GLU | ILE | GLY | VAL | ||||
| 7 | GLU | ALA | PHE | GLN | GLU | TYR | ARG | CYS | ASP | HIS | ||||
| 8 | PRO | VAL | THR | LEU | GLY | MET | ASP | LEU | THR | SER | ||||
| 9 | LEU | SER | LYS | ILE | LEU | ARG | CYS | GLY | ASN | ASN | ||||
| 10 | THR | ASP | THR | LEU | THR | LEU | ILE | ALA | ASP | ASN | ||||
| 11 | THR | PRO | ASP | SER | ILE | ILE | LEU | LEU | PHE | GLU | ||||
| 12 | ASP | THR | LYS | LYS | ASP | ARG | ILE | ALA | GLU | TYR | ||||
| 13 | SER | LEU | LYS | LEU | MET | ASP | ILE | ASP | ALA | ASP | ||||
| 14 | PHE | LEU | LYS | ILE | GLU | GLU | LEU | GLN | TYR | ASP | ||||
| 15 | SER | THR | LEU | SER | LEU | PRO | SER | SER | GLU | PHE | ||||
| 16 | SER | LYS | ILE | VAL | ARG | ASP | LEU | SER | GLN | LEU | ||||
| 17 | SER | ASP | SER | ILE | ASN | ILE | MET | ILE | THR | LYS | ||||
| 18 | GLU | THR | ILE | LYS | PHE | VAL | ALA | ASP | GLY | ASP | ||||
| 19 | ILE | GLY | SER | GLY | SER | VAL | ILE | ILE | LYS | PRO | ||||
| 20 | PHE | VAL | ASP | MET | GLU | HIS | PRO | GLU | THR | SER | ||||
| 21 | ILE | LYS | LEU | GLU | MET | ASP | GLN | PRO | VAL | ASP | ||||
| 22 | LEU | THR | PHE | GLY | ALA | LYS | TYR | LEU | LEU | ASP | ||||
| 23 | ILE | ILE | LYS | GLY | SER | SER | LEU | SER | ASP | ARG | ||||
| 24 | VAL | GLY | ILE | ARG | LEU | SER | SER | GLU | ALA | PRO | ||||
| 25 | ALA | LEU | PHE | GLN | PHE | ASP | LEU | LYS | SER | GLY | ||||
| 26 | PHE | LEU | GLN | PHE | PHE | LEU | ALA | PRO | LYS | PHE | ||||
| 27 | ASN | ASP | GLU | GLU |
Samples:
sample_1: scPCNA monomer, [U-15N; U-13C; U-93% 2H], 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %
sample_2: scPCNA monomer, [U-15N]-Ile, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %
sample_3: scPCNA monomer, [U-15N]-Leu, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %
sample_4: scPCNA monomer, [U-15N]-Phe, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %
sample_5: scPCNA monomer, [U-15N]-Val, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %
conditions_1: pH: 7.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 2D TROSY-1H15N | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D HN(CO)CACB | sample_1 | not available | conditions_1 |
| 3D HN(COCA)CB | sample_1 | not available | conditions_1 |
| 3D HNCA | sample_1 | not available | conditions_1 |
| 3D HN(CO)CA | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D HNCACO | sample_1 | not available | conditions_1 |
| 4D 1H15N_HSQC-NOESY-1H15N-HSQC | sample_1 | not available | conditions_1 |
Software:
NMRPipe, Frank Delaglio -
NMRView v4.0.11, One Moon Scientific -
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks