BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7240

Title: Backbone Assignment of the 98kDa homotrimeric yeast PCNA   PubMed: 17036161

Deposition date: 2006-07-21 Original release date: 2006-11-06

Authors: Campos-Olivas, Ramon; Sanchez, Ricardo; Torres, Daniel; Blanco, Francisco

Citation: Campos-Olivas, Ramon; Sanchez, Ricardo; Torres, Daniel; Blanco, Francisco. "Backbone Assignment of the 98kDa homotrimeric yeast PCNA ring"  J. Biomol. NMR 38, 167-167 (2006).

Assembly members:
scPCNA monomer, polymer, 264 residues, 32635 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
scPCNA monomer: GPHMASMLEAKFEEASLFKR IIDGFKDCVQLVNFQCKEDG IIAQAVDDSRVLLVSLEIGV EAFQEYRCDHPVTLGMDLTS LSKILRCGNNTDTLTLIADN TPDSIILLFEDTKKDRIAEY SLKLMDIDADFLKIEELQYD STLSLPSSEFSKIVRDLSQL SDSINIMITKETIKFVADGD IGSGSVIIKPFVDMEHPETS IKLEMDQPVDLTFGAKYLLD IIKGSSLSDRVGIRLSSEAP ALFQFDLKSGFLQFFLAPKF NDEE

Data sets:
Data typeCount
13C chemical shifts768
15N chemical shifts250
1H chemical shifts251

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1scPCNA monomer 11
2scPCNA monomer 21
3scPCNA monomer 31

Entities:

Entity 1, scPCNA monomer 1 264 residues - 32635 Da.

1   GLYPROHISMETALASERMETLEUGLUALA
2   LYSPHEGLUGLUALASERLEUPHELYSARG
3   ILEILEASPGLYPHELYSASPCYSVALGLN
4   LEUVALASNPHEGLNCYSLYSGLUASPGLY
5   ILEILEALAGLNALAVALASPASPSERARG
6   VALLEULEUVALSERLEUGLUILEGLYVAL
7   GLUALAPHEGLNGLUTYRARGCYSASPHIS
8   PROVALTHRLEUGLYMETASPLEUTHRSER
9   LEUSERLYSILELEUARGCYSGLYASNASN
10   THRASPTHRLEUTHRLEUILEALAASPASN
11   THRPROASPSERILEILELEULEUPHEGLU
12   ASPTHRLYSLYSASPARGILEALAGLUTYR
13   SERLEULYSLEUMETASPILEASPALAASP
14   PHELEULYSILEGLUGLULEUGLNTYRASP
15   SERTHRLEUSERLEUPROSERSERGLUPHE
16   SERLYSILEVALARGASPLEUSERGLNLEU
17   SERASPSERILEASNILEMETILETHRLYS
18   GLUTHRILELYSPHEVALALAASPGLYASP
19   ILEGLYSERGLYSERVALILEILELYSPRO
20   PHEVALASPMETGLUHISPROGLUTHRSER
21   ILELYSLEUGLUMETASPGLNPROVALASP
22   LEUTHRPHEGLYALALYSTYRLEULEUASP
23   ILEILELYSGLYSERSERLEUSERASPARG
24   VALGLYILEARGLEUSERSERGLUALAPRO
25   ALALEUPHEGLNPHEASPLEULYSSERGLY
26   PHELEUGLNPHEPHELEUALAPROLYSPHE
27   ASNASPGLUGLU

Samples:

sample_1: scPCNA monomer, [U-15N; U-13C; U-93% 2H], 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %

sample_2: scPCNA monomer, [U-15N]-Ile, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %

sample_3: scPCNA monomer, [U-15N]-Leu, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %

sample_4: scPCNA monomer, [U-15N]-Phe, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %

sample_5: scPCNA monomer, [U-15N]-Val, 1 ± 0.1 mM; Na2HPO4 10 mM; KH2PO4 2 mM; NaCl 137 mM; KCl 2.7 mM; D2O 8 ± 1 %

conditions_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N_HSQCsample_1not availableconditions_1
2D TROSY-1H15Nsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D HN(CO)CACBsample_1not availableconditions_1
3D HN(COCA)CBsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D HN(CO)CAsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D HNCACOsample_1not availableconditions_1
4D 1H15N_HSQC-NOESY-1H15N-HSQCsample_1not availableconditions_1

Software:

NMRPipe, Frank Delaglio -

NMRView v4.0.11, One Moon Scientific -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts