BMRB Entry 7292
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7292
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Title: Backbone assignment of internal interaction site of ALP PubMed: 19636834
Deposition date: 2006-09-08 Original release date: 2007-08-22
Authors: Alho, Nanna; Klaavuniemi, Tuula; Ylanne, Jari; Permi, Perttu; Mattila, Sampo
Citation: Alho, Nanna; Klaavuniemi, Tuula; Ylanne, Jari; Permi, Perttu; Mattila, Sampo. "Backbone NMR assignment of the internal interaction site of ALP" Biomol. NMR Assignments 1, 85-87 (2007).
Assembly members:
protein, polymer, 171 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
protein: GAMGSEPQDGNYFEHKHNIR
PKPFVIPGRSSGCSTPSGID
CGSGRSTPSSVSTVSTICPG
DLKVAAKLAPNIPLEMELPG
VKIVHAQFNTPMQLYSDDNI
METLQGQVSTALGETPLMSE
PTASVPPESDVYRMLHDNRN
EPTQPRQSGSFRVLQGMVDD
GSDDRPAGTRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 484 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ALP | 1 |
Entities:
Entity 1, ALP 171 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | SER | GLU | PRO | GLN | ASP | GLY | ||||
| 2 | ASN | TYR | PHE | GLU | HIS | LYS | HIS | ASN | ILE | ARG | ||||
| 3 | PRO | LYS | PRO | PHE | VAL | ILE | PRO | GLY | ARG | SER | ||||
| 4 | SER | GLY | CYS | SER | THR | PRO | SER | GLY | ILE | ASP | ||||
| 5 | CYS | GLY | SER | GLY | ARG | SER | THR | PRO | SER | SER | ||||
| 6 | VAL | SER | THR | VAL | SER | THR | ILE | CYS | PRO | GLY | ||||
| 7 | ASP | LEU | LYS | VAL | ALA | ALA | LYS | LEU | ALA | PRO | ||||
| 8 | ASN | ILE | PRO | LEU | GLU | MET | GLU | LEU | PRO | GLY | ||||
| 9 | VAL | LYS | ILE | VAL | HIS | ALA | GLN | PHE | ASN | THR | ||||
| 10 | PRO | MET | GLN | LEU | TYR | SER | ASP | ASP | ASN | ILE | ||||
| 11 | MET | GLU | THR | LEU | GLN | GLY | GLN | VAL | SER | THR | ||||
| 12 | ALA | LEU | GLY | GLU | THR | PRO | LEU | MET | SER | GLU | ||||
| 13 | PRO | THR | ALA | SER | VAL | PRO | PRO | GLU | SER | ASP | ||||
| 14 | VAL | TYR | ARG | MET | LEU | HIS | ASP | ASN | ARG | ASN | ||||
| 15 | GLU | PRO | THR | GLN | PRO | ARG | GLN | SER | GLY | SER | ||||
| 16 | PHE | ARG | VAL | LEU | GLN | GLY | MET | VAL | ASP | ASP | ||||
| 17 | GLY | SER | ASP | ASP | ARG | PRO | ALA | GLY | THR | ARG | ||||
| 18 | SER |
Samples:
ALP_asp: protein ALP, [U-13C; U-15N], 15 mM; amino acid aspartic acid 40 mM; buffer TRIS 20 mM; buffer NaCl 150 ± 5 mM
conditions_1: ionic strength: 150 mM; pH: 4; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | ALP_asp | not available | conditions_1 |
| HNCA | ALP_asp | not available | conditions_1 |
| HNCOCA | ALP_asp | not available | conditions_1 |
| HNCO | ALP_asp | not available | conditions_1 |
| HNCACO | ALP_asp | not available | conditions_1 |
| HNCACB | ALP_asp | not available | conditions_1 |
| CBCACONH | ALP_asp | not available | conditions_1 |
| iHNCA | ALP_asp | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Innova 800 MHz
Related Database Links:
| DBJ | BAD96686 BAG36063 |
| GB | AAB96665 AAC16670 EAX04641 EHH26344 EHH54109 |
| REF | NP_055291 XP_002815395 XP_003823529 XP_003899472 XP_004040756 |
| SP | Q53GG5 |
| AlphaFold | Q53GG5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts