# 1 "/u/exp/stan/nmr/lists/pp/ko_cryo_hcchdigp3d2.2" ;hcchdigp3d2 ;avance-version (01/05/21) ;HCCH-TOCSY ;3D sequence with ; inverse correlation using multiple inept transfer and ; C-C DIPSI3 spinlock ; ; F1(H) -> F2(C,t1) -> F2(C',t2) -> F1(H',t3) ; ;off resonance C=O pulse using shaped pulse ;phase sensitive (t1) ;phase sensitive (t2) ; ;(L.E. Kay, G.Y. Xu, A.U. Singer, D.R. Muhandiram & J. D. Forman-Kay ; J. Magn. Reson. B 101, 333 - 337 (1993)) ;prosol relations= # 1 "/u/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance.incl ; ;avance-version (03/02/17) ;$Id: pulseprogram,v 1.1 2021/02/10 23:28:59 bmrbsvc Exp $ # 21 "/u/exp/stan/nmr/lists/pp/ko_cryo_hcchdigp3d2.2" 2 # 1 "/u/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad.incl - include file for Gradient Spectroscopy ; ;avance-version (02/05/31) define list EA= ;$Id: pulseprogram,v 1.1 2021/02/10 23:28:59 bmrbsvc Exp $ # 22 "/u/exp/stan/nmr/lists/pp/ko_cryo_hcchdigp3d2.2" 2 # 1 "/u/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: pulseprogram,v 1.1 2021/02/10 23:28:59 bmrbsvc Exp $ # 23 "/u/exp/stan/nmr/lists/pp/ko_cryo_hcchdigp3d2.2" 2 "p2=p1*2" "p4=p3*2" "p22=p21*2" "d0=3u" "d10=3u" "d11=30m" "d12=20u" "d4=1.6m" ;tau a "d21=1.1m" ;tau c "d23=475u" ;tau b ;p16:500u ;p19:2m ;p29:300u ;p30:5m ;p31:4.4m "DELTA1=d4-p16-d16-4u" "DELTA2=d23-p29-d16" "DELTA3=p22+p2+d0*2+4u" "DELTA4=d21-p16-d16-4u" "DELTA5=d4-p16-d16-p3*2-52u" "DELTA6=d21+p22+d10*2-p16-d16-4u" "CEN_HC1=(p3-p1)/2" "CEN_HC2=(p4-p2)/2" "CEN_HN2=(p22-p2)/2" "l3=td1/2" "l4=td2/2" "spoff5=bf2*(cnst21/1000000)-o2" ;aqseq 312 1 d11 ze d11 pl12:f2 2 d11 do:f2 d11 3 d11 d11 4 d11 d11 5 d11 d11 6 20u pl9:f1 d1 cw:f1 ph29 4u do:f1 50u setnmr2|0 setnmr0|34|32|33 d12 pl1:f1 d12 pl2:f2 (p3 ph1):f2 4u p18:gp0 d16 200u (p1 ph1) 4u p16:gp1 d16 DELTA1 pl2:f2 (CEN_HC2 p2 ph1) (p4 ph1):f2 4u p16:gp1 d16 DELTA1 pl3:f3 (p1 ph2) p19:gp3 d16 100u (p3 ph3):f2 d0 (p22 ph1):f3 4u p29:gp2 d16 DELTA2 pl0:f2 (p14:sp5 ph1):f2 4u p29:gp2 d16 DELTA2 pl2:f2 p2 ph1 d0 (p4 ph1):f2 DELTA3 p29:gp2 d16 DELTA2 (p14:sp5 ph1):f2 4u p29:gp2 d16 ;pl7:f1 DELTA2 pl2:f2 (p3 ph12):f2 ; 4u ; p17:gp6 ; 100u pl15:f2 20u pl15:f2 9 (p9*2.722 ph7):f2 (p9*4.389 ph9):f2 (p9*2.778 ph7):f2 (p9*3.056 ph9):f2 (p9*0.333 ph7):f2 (p9*2.556 ph9):f2 (p9*4.000 ph7):f2 (p9*2.722 ph9):f2 (p9*4.111 ph7):f2 (p9*3.778 ph9):f2 (p9*3.889 ph7):f2 (p9*2.889 ph9):f2 (p9*3.000 ph7):f2 (p9*0.333 ph9):f2 (p9*2.500 ph7):f2 (p9*4.050 ph9):f2 (p9*2.830 ph7):f2 (p9*4.389 ph9):f2 (p9*2.722 ph9):f2 (p9*4.389 ph7):f2 (p9*2.778 ph9):f2 (p9*3.056 ph7):f2 (p9*0.333 ph9):f2 (p9*2.556 ph7):f2 (p9*4.000 ph9):f2 (p9*2.722 ph7):f2 (p9*4.111 ph9):f2 (p9*3.778 ph7):f2 (p9*3.889 ph9):f2 (p9*2.889 ph7):f2 (p9*3.000 ph9):f2 (p9*0.333 ph7):f2 (p9*2.500 ph9):f2 (p9*4.050 ph7):f2 (p9*2.830 ph9):f2 (p9*4.389 ph7):f2 (p9*2.722 ph9):f2 (p9*4.389 ph7):f2 (p9*2.778 ph9):f2 (p9*3.056 ph7):f2 (p9*0.333 ph9):f2 (p9*2.556 ph7):f2 (p9*4.000 ph9):f2 (p9*2.722 ph7):f2 (p9*4.111 ph9):f2 (p9*3.778 ph7):f2 (p9*3.889 ph9):f2 (p9*2.889 ph7):f2 (p9*3.000 ph9):f2 (p9*0.333 ph7):f2 (p9*2.500 ph9):f2 (p9*4.050 ph7):f2 (p9*2.830 ph9):f2 (p9*4.389 ph7):f2 (p9*2.722 ph7):f2 (p9*4.389 ph9):f2 (p9*2.778 ph7):f2 (p9*3.056 ph9):f2 (p9*0.333 ph7):f2 (p9*2.556 ph9):f2 (p9*4.000 ph7):f2 (p9*2.722 ph9):f2 (p9*4.111 ph7):f2 (p9*3.778 ph9):f2 (p9*3.889 ph7):f2 (p9*2.889 ph9):f2 (p9*3.000 ph7):f2 (p9*0.333 ph9):f2 (p9*2.500 ph7):f2 (p9*4.050 ph9):f2 (p9*2.830 ph7):f2 (p9*4.389 ph9):f2 lo to 9 times l1 ;end DIPSI3 ; d12 ; (p28 ph1) ; 4u ; (p28*2 ph2) 4u p30:gp4 d16 pl1:f1 200u (p1 ph1) 4u p31:gp5 d16 pl2:f2 200u (p3 ph4):f2 4u p16:gp1 d16 DELTA4 pl2:f2 d10 (CEN_HN2 p2 ph1) (p22 ph1):f3 d10 (p4 ph1):f2 4u p16:gp1 d16 DELTA6 (CEN_HC1 p1 ph1) (p3 ph1):f2 4u p16:gp1 d16 DELTA1 (CEN_HC2 p2 ph1) (p4 ph1):f2 4u p16:gp1 d16 DELTA5 20u setnmr2^0 setnmr0^34^32^33 (p3 ph1 4u p3 ph5):f2 4u 20u pl12:f2 (p1 ph1):f1 ;----------------------------- go=2 ph31 cpd2:f2 ;----------------------------- d11 do:f2 wr #0 if #0 zd d11 ip3 lo to 3 times 2 d11 id0 d11 ip31*2 lo to 4 times l3 d11 rd0 d11 ip4 lo to 5 times 2 d11 id10 d11 ip31*2 lo to 6 times l4 d11 setnmr2^0 setnmr0^34^32^33 exit ph29=0 ph1=0 ph2=1 ph3=0 2 ph4=1 1 3 3 ph5=0 0 0 0 2 2 2 2 ph7=1 ph9=3 ph12=1 1 1 1 3 3 3 3 ph31=0 2 2 0 2 0 0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl12: f2 channel - power level for CPD/BB decoupling ;pl15: f2 channel - power level for TOCSY-spinlock ;sp5: f2 channel - shaped pulse 180 degree (C=O off resonance) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p4 : f2 channel - 180 degree high power pulse ;p9 : f2 channel - 90 degree low power pulse ;p14: f2 channel - 180 degree shaped pulse ;p16: homospoil/gradient pulse [500 usec] ;p19: gradient pulse 2 [2 msec] ;p22: f3 channel - 180 degree high power pulse ;p28: f1 channel - trim pulse [1 msec] ;p29: gradient pulse 3 [300 usec] ;p30: gradient pulse 4 [5 msec] ;p31: gradient pulse 5 [4.4 msec] ;d0 : incremented delay (F1 in 3D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d4 : 1/(4J(CH)) - tau a [1.6 msec] ;d10: incremented delay (F2 in 3D) [3 usec] ;d11: delay for disk I/O [30 msec] ;d12: delay for power switching [20 usec] ;d16: delay for homospoil/gradient recovery ;d21: 1/(6J'(CH)) - tau c [1.1 msec] ;d23: tau b [475 usec] ;l1: loop for DIPSI cycle: ; mixing time = ((p9*54.33*4) * l1) [12 msec] ;in0: 1/(2 * SW(C)) = DW(C) ;nd0: 2 ;in10: 1/(2 * SW(C)) = DW(C) ;nd10: 2 ;NS: 8 * n ;DS: 32 ;td1: number of experiments in F1 ;td2: number of experiments in F2 ;FnMODE: States-TPPI (or TPPI) in F1 ;FnMODE: States-TPPI (or TPPI) in F2 ;cpd2: decoupling according to sequence defined by cpdprg2 ;pcpd2: f2 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 : gp 4 ; 16 : 16 : 30 : 60 ;for z-only gradients: ;gpz1: 16% ;gpz2: 16% ;gpz3: 30% ;gpz4: 60% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.50 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;$Id: pulseprogram,v 1.1 2021/02/10 23:28:59 bmrbsvc Exp $