data_15379 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Sup35 NM ; _BMRB_accession_number 15379 _BMRB_flat_file_name bmr15379.str _Entry_type original _Submission_date 2007-07-13 _Accession_date 2007-07-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Toyama Brandon H. . 2 Kelly Mark JS . 3 Gross John D. . 4 Weissman Jonathan S. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 132 "15N chemical shifts" 132 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2008-07-03 update BMRB 'complete entry citation' 2007-09-12 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'The structural basis of yeast prion strain variants' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17767153 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Toyama Brandon H. . 2 Kelly Mark JS . 3 Gross John D. . 4 Weissman Jonathan S. . stop_ _Journal_abbreviation Nature _Journal_volume 449 _Journal_issue 7159 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 233 _Page_last 237 _Year 2007 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Sup35 NM domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Sup35 NM domain' $Sup35-NM stop_ _System_molecular_weight . _System_physical_state denatured _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Sup35-NM _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Sup35-NM _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 259 _Mol_residue_sequence ; MSDSNQGNNQQNYQQYSQNG NQQQGNNRYQGYQAYNAQAQ PAGGYYQNYQGYSGYQQGGY QQYNPDAGYQQQYNPQGGYQ QYNPQGGYQQQFNPQGGRGN YKNFNYNNNLQGYQAGFQPQ SQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKK VGTKPAESDKKEEEKSAETK EPTKEPTKVEEPVKKEEKPV QTEEKTEEKSELPKVEDLKI SESTHNTNNANVTSADALIK EQEEEVDDEVVNDHHHHHH ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 ASP 4 SER 5 ASN 6 GLN 7 GLY 8 ASN 9 ASN 10 GLN 11 GLN 12 ASN 13 TYR 14 GLN 15 GLN 16 TYR 17 SER 18 GLN 19 ASN 20 GLY 21 ASN 22 GLN 23 GLN 24 GLN 25 GLY 26 ASN 27 ASN 28 ARG 29 TYR 30 GLN 31 GLY 32 TYR 33 GLN 34 ALA 35 TYR 36 ASN 37 ALA 38 GLN 39 ALA 40 GLN 41 PRO 42 ALA 43 GLY 44 GLY 45 TYR 46 TYR 47 GLN 48 ASN 49 TYR 50 GLN 51 GLY 52 TYR 53 SER 54 GLY 55 TYR 56 GLN 57 GLN 58 GLY 59 GLY 60 TYR 61 GLN 62 GLN 63 TYR 64 ASN 65 PRO 66 ASP 67 ALA 68 GLY 69 TYR 70 GLN 71 GLN 72 GLN 73 TYR 74 ASN 75 PRO 76 GLN 77 GLY 78 GLY 79 TYR 80 GLN 81 GLN 82 TYR 83 ASN 84 PRO 85 GLN 86 GLY 87 GLY 88 TYR 89 GLN 90 GLN 91 GLN 92 PHE 93 ASN 94 PRO 95 GLN 96 GLY 97 GLY 98 ARG 99 GLY 100 ASN 101 TYR 102 LYS 103 ASN 104 PHE 105 ASN 106 TYR 107 ASN 108 ASN 109 ASN 110 LEU 111 GLN 112 GLY 113 TYR 114 GLN 115 ALA 116 GLY 117 PHE 118 GLN 119 PRO 120 GLN 121 SER 122 GLN 123 GLY 124 MET 125 SER 126 LEU 127 ASN 128 ASP 129 PHE 130 GLN 131 LYS 132 GLN 133 GLN 134 LYS 135 GLN 136 ALA 137 ALA 138 PRO 139 LYS 140 PRO 141 LYS 142 LYS 143 THR 144 LEU 145 LYS 146 LEU 147 VAL 148 SER 149 SER 150 SER 151 GLY 152 ILE 153 LYS 154 LEU 155 ALA 156 ASN 157 ALA 158 THR 159 LYS 160 LYS 161 VAL 162 GLY 163 THR 164 LYS 165 PRO 166 ALA 167 GLU 168 SER 169 ASP 170 LYS 171 LYS 172 GLU 173 GLU 174 GLU 175 LYS 176 SER 177 ALA 178 GLU 179 THR 180 LYS 181 GLU 182 PRO 183 THR 184 LYS 185 GLU 186 PRO 187 THR 188 LYS 189 VAL 190 GLU 191 GLU 192 PRO 193 VAL 194 LYS 195 LYS 196 GLU 197 GLU 198 LYS 199 PRO 200 VAL 201 GLN 202 THR 203 GLU 204 GLU 205 LYS 206 THR 207 GLU 208 GLU 209 LYS 210 SER 211 GLU 212 LEU 213 PRO 214 LYS 215 VAL 216 GLU 217 ASP 218 LEU 219 LYS 220 ILE 221 SER 222 GLU 223 SER 224 THR 225 HIS 226 ASN 227 THR 228 ASN 229 ASN 230 ALA 231 ASN 232 VAL 233 THR 234 SER 235 ALA 236 ASP 237 ALA 238 LEU 239 ILE 240 LYS 241 GLU 242 GLN 243 GLU 244 GLU 245 GLU 246 VAL 247 ASP 248 ASP 249 GLU 250 VAL 251 VAL 252 ASN 253 ASP 254 HIS 255 HIS 256 HIS 257 HIS 258 HIS 259 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-03-16 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 18406 Sup35NM 97.68 273 100.00 100.00 2.29e-174 BMRB 18407 Sup35 97.68 705 100.00 100.00 9.25e-172 DBJ GAA22402 "K7_Sup35p [Saccharomyces cerevisiae Kyokai no. 7]" 97.68 685 98.02 98.42 7.08e-167 EMBL CAA30155 "unnamed protein product [Saccharomyces cerevisiae]" 97.68 685 100.00 100.00 5.29e-172 EMBL CAA68760 "GST1 [Saccharomyces cerevisiae]" 97.68 685 99.60 99.60 1.19e-171 EMBL CAA86677 "Sup2p [Saccharomyces cerevisiae]" 97.68 685 100.00 100.00 5.29e-172 EMBL CAY78675 "Sup35p [Saccharomyces cerevisiae EC1118]" 97.68 685 98.81 99.21 4.05e-169 GB AAA35133 "omnipotent suppressor (alt.) [Saccharomyces cerevisiae]" 97.68 685 100.00 100.00 5.29e-172 GB AAF99684 "SUP35 allosuppressor mutant sal3-4 [Saccharomyces cerevisiae]" 97.68 685 100.00 100.00 4.60e-172 GB AAK26176 "prion protein [Saccharomyces cerevisiae]" 97.68 685 99.60 99.60 5.11e-171 GB AAK26180 "prion protein [Saccharomyces cerevisiae]" 97.68 685 99.60 99.60 5.11e-171 GB AAK26182 "prion protein, partial [Saccharomyces cerevisiae]" 93.44 242 99.59 99.59 1.94e-164 REF NP_010457 "translation termination factor GTPase eRF3 [Saccharomyces cerevisiae S288c]" 97.68 685 100.00 100.00 5.29e-172 SP P05453 "RecName: Full=Eukaryotic peptide chain release factor GTP-binding subunit; AltName: Full=ERF-3; Short=ERF3; AltName: Full=ERF2;" 97.68 685 100.00 100.00 5.29e-172 TPG DAA12014 "TPA: translation termination factor GTPase eRF3 [Saccharomyces cerevisiae S288c]" 97.68 685 100.00 100.00 5.29e-172 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Sup35-NM 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Sup35-NM 'recombinant technology' . Escherichia coli . pAED-SupNM stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Sup35-NM 100-300 uM [U-15N] DMSO 95 % 'natural abundance' H2O 4.5 % 'natural abundance' 'dichloroacetic acid-d2' 0.5 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_ANSIG _Saveframe_category software _Name ANSIG _Version 3.3 loop_ _Vendor _Address _Electronic_address Kraulis . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HN(COCA)CB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(COCA)CB' _Sample_label $sample_1 save_ save_HN(CA)NH_6 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CA)NH _Sample_label $sample_1 save_ save_CT-HNCA_7 _Saveframe_category NMR_applied_experiment _Experiment_name CT-HNCA _Sample_label $sample_1 save_ save_HN(CA)CO_8 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CA)CO _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 . pH pressure 1 . atm temperature 300 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 external indirect . . . 1 DSS N 15 'methyl protons' ppm 0 external indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $ANSIG stop_ loop_ _Experiment_label '3D HNCO' '3D HNCACB' '3D CBCA(CO)NH' '3D HN(COCA)CB' HN(CA)NH CT-HNCA HN(CA)CO stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Sup35 NM domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 SER H H 7.999 0.008 . 2 4 4 SER N N 113.901 0.02 . 3 5 5 ASN H H 8.228 0.008 . 4 5 5 ASN N N 120.177 0.02 . 5 7 7 GLY H H 8.185 0.008 . 6 7 7 GLY N N 107.151 0.02 . 7 8 8 ASN H H 8.113 0.008 . 8 8 8 ASN N N 117.832 0.02 . 9 9 9 ASN H H 8.241 0.008 . 10 9 9 ASN N N 118.991 0.02 . 11 11 11 GLN H H 7.958 0.008 . 12 11 11 GLN N N 118.199 0.02 . 13 15 15 GLN H H 7.943 0.008 . 14 15 15 GLN N N 119.035 0.02 . 15 16 16 TYR H H 7.942 0.008 . 16 16 16 TYR N N 118.423 0.02 . 17 17 17 SER H H 8.125 0.008 . 18 17 17 SER N N 115.001 0.02 . 19 18 18 GLN H H 8.144 0.008 . 20 18 18 GLN N N 120.658 0.02 . 21 19 19 ASN H H 8.138 0.008 . 22 19 19 ASN N N 118.33 0.02 . 23 22 22 GLN H H 8.09 0.008 . 24 22 22 GLN N N 118.997 0.02 . 25 23 23 GLN H H 8.008 0.008 . 26 23 23 GLN N N 118.731 0.02 . 27 24 24 GLN H H 7.972 0.008 . 28 24 24 GLN N N 118.928 0.02 . 29 25 25 GLY H H 8.131 0.008 . 30 25 25 GLY N N 107.437 0.02 . 31 26 26 ASN H H 8.161 0.008 . 32 26 26 ASN N N 118.157 0.02 . 33 27 27 ASN H H 8.199 0.008 . 34 27 27 ASN N N 118.866 0.02 . 35 30 30 GLN H H 7.911 0.008 . 36 30 30 GLN N N 119.052 0.02 . 37 31 31 GLY H H 7.948 0.008 . 38 31 31 GLY N N 107.363 0.02 . 39 33 33 GLN H H 8.244 0.008 . 40 33 33 GLN N N 119.607 0.02 . 41 34 34 ALA H H 7.956 0.008 . 42 34 34 ALA N N 122.342 0.02 . 43 35 35 TYR H H 7.879 0.008 . 44 35 35 TYR N N 116.5 0.02 . 45 36 36 ASN H H 8.227 0.008 . 46 36 36 ASN N N 118.888 0.02 . 47 37 37 ALA H H 8.08 0.008 . 48 37 37 ALA N N 122.743 0.02 . 49 38 38 GLN H H 7.99 0.008 . 50 38 38 GLN N N 116.286 0.02 . 51 39 39 ALA H H 7.773 0.008 . 52 39 39 ALA N N 121.483 0.02 . 53 42 42 ALA H H 8.152 0.008 . 54 42 42 ALA N N 121.588 0.02 . 55 43 43 GLY H H 8.047 0.008 . 56 43 43 GLY N N 105.532 0.02 . 57 44 44 GLY H H 7.976 0.008 . 58 44 44 GLY N N 106.222 0.02 . 59 45 45 TYR H H 7.999 0.008 . 60 45 45 TYR N N 117.975 0.02 . 61 51 51 GLY H H 7.905 0.008 . 62 51 51 GLY N N 107.076 0.02 . 63 53 53 SER H H 8.194 0.008 . 64 53 53 SER N N 115.483 0.02 . 65 54 54 GLY H H 8.012 0.008 . 66 54 54 GLY N N 108.413 0.02 . 67 55 55 TYR H H 7.948 0.008 . 68 55 55 TYR N N 117.865 0.02 . 69 59 59 GLY H H 8.077 0.008 . 70 59 59 GLY N N 106.548 0.02 . 71 66 66 ASP H H 7.95 0.008 . 72 66 66 ASP N N 115.021 0.02 . 73 67 67 ALA H H 7.316 0.008 . 74 67 67 ALA N N 120.641 0.02 . 75 68 68 GLY H H 7.969 0.008 . 76 68 68 GLY N N 105.144 0.02 . 77 69 69 TYR H H 7.917 0.008 . 78 69 69 TYR N N 117.612 0.02 . 79 70 70 GLN H H 8.189 0.008 . 80 70 70 GLN N N 119.179 0.02 . 81 76 76 GLN H H 7.964 0.008 . 82 76 76 GLN N N 116.215 0.02 . 83 77 77 GLY H H 7.751 0.008 . 84 77 77 GLY N N 105.862 0.02 . 85 78 78 GLY H H 7.946 0.008 . 86 78 78 GLY N N 106.138 0.02 . 87 85 85 GLN H H 7.964 0.008 . 88 85 85 GLN N N 116.215 0.02 . 89 86 86 GLY H H 7.751 0.008 . 90 86 86 GLY N N 105.862 0.02 . 91 87 87 GLY H H 7.946 0.008 . 92 87 87 GLY N N 106.138 0.02 . 93 95 95 GLN H H 8.024 0.008 . 94 95 95 GLN N N 116.353 0.02 . 95 96 96 GLY H H 7.836 0.008 . 96 96 96 GLY N N 106.222 0.02 . 97 97 97 GLY H H 8.022 0.008 . 98 97 97 GLY N N 106.422 0.02 . 99 99 99 GLY H H 8.257 0.008 . 100 99 99 GLY N N 107.626 0.02 . 101 110 110 LEU H H 7.843 0.008 . 102 110 110 LEU N N 118.881 0.02 . 103 111 111 GLN H H 7.843 0.008 . 104 111 111 GLN N N 117.905 0.02 . 105 112 112 GLY H H 8 0.008 . 106 112 112 GLY N N 106.974 0.02 . 107 114 114 GLN H H 8.186 0.008 . 108 114 114 GLN N N 119.412 0.02 . 109 115 115 ALA H H 7.988 0.008 . 110 115 115 ALA N N 122.339 0.02 . 111 116 116 GLY H H 8.071 0.008 . 112 116 116 GLY N N 105.487 0.02 . 113 117 117 PHE H H 7.959 0.008 . 114 117 117 PHE N N 117.217 0.02 . 115 118 118 GLN H H 8.336 0.008 . 116 118 118 GLN N N 120.951 0.02 . 117 120 120 GLN H H 8.278 0.008 . 118 120 120 GLN N N 118.439 0.02 . 119 121 121 SER H H 7.879 0.008 . 120 121 121 SER N N 114.651 0.02 . 121 123 123 GLY H H 8.13 0.008 . 122 123 123 GLY N N 107.099 0.02 . 123 124 124 MET H H 8.016 0.008 . 124 124 124 MET N N 117.786 0.02 . 125 125 125 SER H H 8.093 0.008 . 126 125 125 SER N N 116.039 0.02 . 127 126 126 LEU H H 8.042 0.008 . 128 126 126 LEU N N 121.725 0.02 . 129 127 127 ASN H H 8.116 0.008 . 130 127 127 ASN N N 117.181 0.02 . 131 128 128 ASP H H 8.253 0.008 . 132 128 128 ASP N N 118.611 0.02 . 133 129 129 PHE H H 7.971 0.008 . 134 129 129 PHE N N 116.972 0.02 . 135 130 130 GLN H H 7.926 0.008 . 136 130 130 GLN N N 118.716 0.02 . 137 131 131 LYS H H 7.985 0.008 . 138 131 131 LYS N N 119.702 0.02 . 139 136 136 ALA H H 8.065 0.008 . 140 136 136 ALA N N 122.623 0.02 . 141 141 141 LYS H H 8.154 0.008 . 142 141 141 LYS N N 118.879 0.02 . 143 143 143 THR H H 7.831 0.008 . 144 143 143 THR N N 113.412 0.02 . 145 144 144 LEU H H 7.954 0.008 . 146 144 144 LEU N N 121.864 0.02 . 147 146 146 LEU H H 8.071 0.008 . 148 146 146 LEU N N 121.108 0.02 . 149 147 147 VAL H H 7.716 0.008 . 150 147 147 VAL N N 115.633 0.02 . 151 148 148 SER H H 8.089 0.008 . 152 148 148 SER N N 117.308 0.02 . 153 149 149 SER H H 8.064 0.008 . 154 149 149 SER N N 116.368 0.02 . 155 150 150 SER H H 8.093 0.008 . 156 150 150 SER N N 116.039 0.02 . 157 151 151 GLY H H 8.124 0.008 . 158 151 151 GLY N N 108.514 0.02 . 159 152 152 ILE H H 7.804 0.008 . 160 152 152 ILE N N 117.337 0.02 . 161 153 153 LYS H H 8.124 0.008 . 162 153 153 LYS N N 123.246 0.02 . 163 154 154 LEU H H 7.968 0.008 . 164 154 154 LEU N N 120.782 0.02 . 165 157 157 ALA H H 7.942 0.008 . 166 157 157 ALA N N 121.451 0.02 . 167 158 158 THR H H 7.807 0.008 . 168 158 158 THR N N 111.18 0.02 . 169 159 159 LYS H H 7.913 0.008 . 170 159 159 LYS N N 120.471 0.02 . 171 160 160 LYS H H 8.103 0.008 . 172 160 160 LYS N N 120.277 0.02 . 173 161 161 VAL H H 7.764 0.008 . 174 161 161 VAL N N 116.394 0.02 . 175 162 162 GLY H H 8.26 0.008 . 176 162 162 GLY N N 109.837 0.02 . 177 163 163 THR H H 7.775 0.008 . 178 163 163 THR N N 111.903 0.02 . 179 166 166 ALA H H 8.18 0.008 . 180 166 166 ALA N N 121.427 0.02 . 181 167 167 GLU H H 7.955 0.008 . 182 167 167 GLU N N 116.8 0.02 . 183 168 168 SER H H 7.942 0.008 . 184 168 168 SER N N 114.265 0.02 . 185 169 169 ASP H H 8.344 0.008 . 186 169 169 ASP N N 120.58 0.02 . 187 170 170 LYS H H 7.862 0.008 . 188 170 170 LYS N N 118.655 0.02 . 189 171 171 LYS H H 8.024 0.008 . 190 171 171 LYS N N 119.607 0.02 . 191 176 176 SER H H 8.061 0.008 . 192 176 176 SER N N 115.178 0.02 . 193 177 177 ALA H H 8.123 0.008 . 194 177 177 ALA N N 123.482 0.02 . 195 178 178 GLU H H 8.076 0.008 . 196 178 178 GLU N N 117.353 0.02 . 197 179 179 THR H H 7.719 0.008 . 198 179 179 THR N N 113.073 0.02 . 199 180 180 LYS H H 7.95 0.008 . 200 180 180 LYS N N 120.366 0.02 . 201 183 183 THR H H 7.885 0.008 . 202 183 183 THR N N 112.084 0.02 . 203 184 184 LYS H H 7.84 0.008 . 204 184 184 LYS N N 119.788 0.02 . 205 187 187 THR H H 7.912 0.008 . 206 187 187 THR N N 112.048 0.02 . 207 188 188 LYS H H 7.821 0.008 . 208 188 188 LYS N N 120.032 0.02 . 209 189 189 VAL H H 7.885 0.008 . 210 189 189 VAL N N 117.111 0.02 . 211 190 190 GLU H H 8.065 0.008 . 212 190 190 GLU N N 121.572 0.02 . 213 193 193 VAL H H 7.912 0.008 . 214 193 193 VAL N N 116.161 0.02 . 215 194 194 LYS H H 7.991 0.008 . 216 194 194 LYS N N 121.746 0.02 . 217 198 198 LYS H H 8.183 0.008 . 218 198 198 LYS N N 120.99 0.02 . 219 200 200 VAL H H 7.916 0.008 . 220 200 200 VAL N N 116.292 0.02 . 221 201 201 GLN H H 8.129 0.008 . 222 201 201 GLN N N 121.689 0.02 . 223 202 202 THR H H 7.821 0.008 . 224 202 202 THR N N 113.288 0.02 . 225 203 203 GLU H H 7.917 0.008 . 226 203 203 GLU N N 119.702 0.02 . 227 204 204 GLU H H 8.12 0.008 . 228 204 204 GLU N N 119.761 0.02 . 229 205 205 LYS H H 8.102 0.008 . 230 205 205 LYS N N 120.591 0.02 . 231 206 206 THR H H 7.888 0.008 . 232 206 206 THR N N 113.643 0.02 . 233 207 207 GLU H H 7.952 0.008 . 234 207 207 GLU N N 119.937 0.02 . 235 214 214 LYS H H 8.119 0.008 . 236 214 214 LYS N N 118.836 0.02 . 237 215 215 VAL H H 7.668 0.008 . 238 215 215 VAL N N 115.839 0.02 . 239 216 216 GLU H H 8.091 0.008 . 240 216 216 GLU N N 120.68 0.02 . 241 218 218 LEU H H 7.881 0.008 . 242 218 218 LEU N N 119.93 0.02 . 243 220 220 ILE H H 7.727 0.008 . 244 220 220 ILE N N 117.584 0.02 . 245 221 221 SER H H 8.042 0.008 . 246 221 221 SER N N 117.738 0.02 . 247 222 222 GLU H H 8.018 0.008 . 248 222 222 GLU N N 120.093 0.02 . 249 223 223 SER H H 8.034 0.008 . 250 223 223 SER N N 115.061 0.02 . 251 224 224 THR H H 7.875 0.008 . 252 224 224 THR N N 113.736 0.02 . 253 225 225 HIS H H 8.14 0.008 . 254 225 225 HIS N N 117.712 0.02 . 255 226 226 ASN H H 8.337 0.008 . 256 226 226 ASN N N 119.721 0.02 . 257 227 227 THR H H 7.882 0.008 . 258 227 227 THR N N 112.466 0.02 . 259 229 229 ASN H H 8.102 0.008 . 260 229 229 ASN N N 118.122 0.02 . 261 230 230 ALA H H 8.148 0.008 . 262 230 230 ALA N N 121.994 0.02 . 263 231 231 ASN H H 8.171 0.008 . 264 231 231 ASN N N 117.282 0.02 . stop_ save_