# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" ;hsqct1etf3gpsi3d ;avance-version (07/04/04) ;3D H-1/X correlation via double inept transfer ; using sensitivity improvement ;for measuring N-15 T1 relaxation times ;phase sensitive using Echo/Antiecho-TPPI gradient selection ;with decoupling during acquisition ;using f3 - channel ;using flip-back pulse ;as pseudo3D ;(use parameterset HSQCT1ETF3GPSI3D) ; ;$CLASS=HighRes ;$DIM=3D ;$TYPE= ;$SUBTYPE= ;$COMMENT= prosol relations= # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance2.incl ; for 1 ; ;avance-version (07/08/21) ; ;$CLASS=HighRes Incl ;$COMMENT= ;$Id: pulseprogram,v 1.1 2021/02/10 22:26:23 bmrbsvc Exp $ # 23 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad2.incl - include file for Gradient Spectroscopy ; for 1 ; ;avance-version (07/01/17) ; ;$CLASS=HighRes Incl ;$COMMENT= define list EA= ;$Id: pulseprogram,v 1.1 2021/02/10 22:26:23 bmrbsvc Exp $ # 24 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ; ;$CLASS=HighRes Incl ;$COMMENT= ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: pulseprogram,v 1.1 2021/02/10 22:26:23 bmrbsvc Exp $ # 25 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" 2 "p2=p1*2" "p22=p21*2" "d11=30m" "d24=1s/(cnst4*cnst11)" "d25=1s/(cnst4*cnst12)" "d26=1s/(cnst4*4)" "d10=3u" "in10=inf2/2" "DELTA2=p16+d16+8u" "DELTA3=d25-p16-d16-4u" "DELTA1=d25-p16-d16-p2-d10*2" "spoff1=0" "spoff13=bf2*((cnst21+cnst22)/2000000)-o2" aqseq 312 # 1 "mc_line 57 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d expanding definition part of mc command before ze" ; dimension 3 aq-mode (F2) QF (F1) Echo-Antiecho F2->F1 define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td2 / (2)" "MCWRK = d1" "MCREST = d1 - d1" dccorr # 57 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" 1 ze # 1 "mc_line 57 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d expanding definition of mc command after ze" # 58 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" d11 pl16:f3 st0 2 6m do:f3 3 3m # 1 "mc_line 61 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d expanding start label for mc command" 4 MCWRK MCREST # 62 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" "TAU=vd-p26*2-p16-d16-8u" (p1 ph1) d26 pl3:f3 (center (p2 ph1) (p22 ph1):f3 ) d26 setnmr3|0 setnmr0|34|32|33 ctrlgrad 0 (p1 ph2) 4u pl0:f1 (p11:sp1 ph1:r):f1 4u p16:gp1 d16 pl1:f1 (p21 ph3):f3 d25 (center (p2 ph1) (p22 ph1):f3 ) d25 pl19:f1 (p21 ph2):f3 (p26 ph2):f1 TAU cpds1:f1 ph1 4u do:f1 (p26 ph7):f1 4u p16:gp4 d16 pl1:f1 (p21 ph8):f3 4u p16:gp2*-1*EA d16 DELTA3 (p22 ph6):f3 d10 (p2 ph9) d10 p16:gp2*EA d16 DELTA1 (center (p1 ph1) (p21 ph4):f3 ) d24 (center (p2 ph1) (p22 ph1):f3 ) d24 (center (p1 ph2) (p21 ph5):f3 ) d26 (center (p2 ph1) (p22 ph1):f3 ) d26 (p1 ph1) DELTA2 (p2 ph1) 4u p16:gp3 d16 pl16:f3 4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7 goscnp ph31 cpd3:f3 3m do:f3 3m st ivd lo to 3 times nbl 3m ipp3 ipp4 ipp5 ipp8 ipp9 ipp31 lo to 4 times ns # 1 "mc_line 136 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d expanding mc command in line" MCWRK wr #0 if #0 zd MCWRK # 139 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqct1etf3gpsi3d" exit ph0=0 ph1=0 ph2=1 ph3=0 0 0 0 2 2 2 2 ph4=0 0 2 2 ph5=3 3 1 1 ph6=0 ph7=3 ph8=1 3 ph9=0 0 2 2 ph31=0 2 2 0 2 0 0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl16: f3 channel - power level for CPD/BB decoupling ;pl19: f1 channel - power level for CPD/BB decoupling ;sp1 : f1 channel - shaped pulse 90 degree ;sp13: f2 channel - shaped pulse 180 degree (Ca and C=O, adiabatic) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p8 : f2 channel - 180 degree shaped pulse for inversion (adiabatic) ;p11: f1 channel - 90 degree shaped pulse ;p16: homospoil/gradient pulse ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;p26: f1 channel - 90 degree pulse at pl19 ;d1 : relaxation delay; 1-5 * T1 ;d10: incremented delay [3 usec] ;d11: delay for disk I/O [30 msec] ;d16: delay for homospoil/gradient recovery ;d24: 1/(4J)YH for YH ; 1/(8J)YH for all multiplicities ;d25: 1/(4J)YH for YH ; 1/(8J)YH for all multiplicities ;d26: 1/(4J(YH)) ;vd : variable delay, taken from vd-list ;cnst4: = J(YH) ;cnst11: for multiplicity selection = 4 for NH, 8 for all multiplicities ;cnst12: for multiplicity selection = 4 for NH, 8 for all multiplicities ;cnst21: CO chemical shift (offset, in ppm) ;cnst22: Calpha chemical shift (offset, in ppm) ;inf2: 1/SW(X) = 2 * DW(X) ;in10: 1/(2 * SW(X)) = DW(X) ;nd10: 2 ;NS: 2 * n ;DS: >= 16 ;td1: number of delays in vd-list ;td2: number of experiments in F2 ;NBL: = td1 ;FnMODE: QF in F1 ;FnMODE: echo-antiecho in F2 ;cpds1: decoupling according to sequence defined by cpdprg1 ;cpd3: decoupling according to sequence defined by cpdprg3 ;pcpd1: f1 channel - 90 degree pulse for decoupling sequence ;pcpd3: f3 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 : gp 4 ; 50 : 80 : 16.2 : 30 for N-15 ;for z-only gradients: ;gpz1: 50% ;gpz2: 80% ;gpz3: 16.2% ;gpz4: 30% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;preprocessor-flags-start ;LABEL_CN: for C-13 and N-15 labeled samples start experiment with ; option -DLABEL_CN (eda: ZGOPTNS) ;preprocessor-flags-end ;$Id: pulseprogram,v 1.1 2021/02/10 22:26:23 bmrbsvc Exp $