data_15936 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30% ; _BMRB_accession_number 15936 _BMRB_flat_file_name bmr15936.str _Entry_type original _Submission_date 2008-09-01 _Accession_date 2008-09-01 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'TFE-induced native-like fold of the initially unfolded protein' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kaczka Piotr . . 2 Polkowska Agnieszka . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 10 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 1000 "15N chemical shifts" 1000 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-01-25 update BMRB 'complete entry citation' 2009-11-20 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 15930 'chemical shifts and relaxation parameters of 4 sigma70' 4870 '4 sigma70' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 19847776 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kaczka Piotr . . 2 Polkowska-Nowakowska Agnieszka . . 3 Bolewska Krystyna . . 4 Zhukov Igor . . 5 Poznaski Jarosaw . . 6 Wierzchowski Kazimierz L. . stop_ _Journal_abbreviation Proteins _Journal_name_full Proteins _Journal_volume 78 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 754 _Page_last 768 _Year 2010 _Details . loop_ _Keyword 'E. coli RNA polymerase' 'NMR assignment' sigma70 'TFE titration' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name '4 sigma70' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label '4 sigma70' $4_sigma70 stop_ _System_molecular_weight . _System_physical_state unfolded _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'promoter DNA recognition factor' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_4_sigma70 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 4_sigma70 _Molecular_mass 12032.5 _Mol_thiol_state 'not present' loop_ _Biological_function 'promoter DNA recognition factor' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 107 _Mol_residue_sequence ; MGSSHHHHHHSSGLVPRGSH MLELPLDSATTESLRAATHD VLAGLTAREAKVLRMRFGID MNTDYTLEEVGKQFDVTRER IRQIEAKALRKLRHPSRSEV LRSFLDD ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLY 3 SER 4 SER 5 HIS 6 HIS 7 HIS 8 HIS 9 HIS 10 HIS 11 SER 12 SER 13 GLY 14 LEU 15 VAL 16 PRO 17 ARG 18 GLY 19 SER 20 HIS 21 MET 22 LEU 23 GLU 24 LEU 25 PRO 26 LEU 27 ASP 28 SER 29 ALA 30 THR 31 THR 32 GLU 33 SER 34 LEU 35 ARG 36 ALA 37 ALA 38 THR 39 HIS 40 ASP 41 VAL 42 LEU 43 ALA 44 GLY 45 LEU 46 THR 47 ALA 48 ARG 49 GLU 50 ALA 51 LYS 52 VAL 53 LEU 54 ARG 55 MET 56 ARG 57 PHE 58 GLY 59 ILE 60 ASP 61 MET 62 ASN 63 THR 64 ASP 65 TYR 66 THR 67 LEU 68 GLU 69 GLU 70 VAL 71 GLY 72 LYS 73 GLN 74 PHE 75 ASP 76 VAL 77 THR 78 ARG 79 GLU 80 ARG 81 ILE 82 ARG 83 GLN 84 ILE 85 GLU 86 ALA 87 LYS 88 ALA 89 LEU 90 ARG 91 LYS 92 LEU 93 ARG 94 HIS 95 PRO 96 SER 97 ARG 98 SER 99 GLU 100 VAL 101 LEU 102 ARG 103 SER 104 PHE 105 LEU 106 ASP 107 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-08-12 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15930 4_sigma70 100.00 107 100.00 100.00 2.23e-69 BMRB 15975 4_sigma70 100.00 107 100.00 100.00 2.23e-69 PDB 1TLH "T4 Asia Bound To Sigma70 Region 4" 75.70 81 100.00 100.00 4.77e-49 PDB 2P7V "Crystal Structure Of The Escherichia Coli Regulator Of Sigma 70, Rsd, In Complex With Sigma 70 Domain 4" 63.55 68 100.00 100.00 1.02e-39 PDB 3IYD "Three-Dimensional Em Structure Of An Intact Activator-Dependent Transcription Initiation Complex" 80.37 613 98.84 100.00 1.29e-47 PDB 3T72 "Phob(E)-Sigma70(4)-(Rnap-Betha-Flap-Tip-Helix)-Dna Transcription Activation Sub-Complex" 72.90 99 98.72 100.00 6.92e-46 PDB 4JK1 "X-ray Crystal Structure Of Escherichia Coli Sigma70 Holoenzyme In Complex With Guanosine Tetraphosphate (ppgpp)" 80.37 613 100.00 100.00 9.23e-49 PDB 4JK2 "X-ray Crystal Structure Of Escherichia Coli Sigma70 Holoenzyme In Complex With Guanosine Pentaphosphate (pppgpp)" 80.37 613 100.00 100.00 9.23e-49 PDB 4JKR "Crystal Structure Of E. Coli Rna Polymerase In Complex With Ppgpp" 80.37 628 100.00 100.00 1.74e-48 PDB 4KMU "X-ray Crystal Structure Of The Escherichia Coli Rna Polymerase In Complex With Rifampin" 80.37 613 100.00 100.00 9.23e-49 PDB 4KN4 "X-ray Crystal Structure Of The Escherichia Coli Rna Polymerase In Complex With Benzoxazinorifamycin-2b" 80.37 613 100.00 100.00 9.23e-49 PDB 4KN7 "X-ray Crystal Structure Of The Escherichia Coli Rna Polymerase In Complex With Benzoxazinorifamycin-2c" 80.37 613 100.00 100.00 9.23e-49 PDB 4LJZ "Crystal Structure Analysis Of The E.coli Holoenzyme" 80.37 522 100.00 100.00 6.32e-49 PDB 4LK0 "Crystal Structure Analysis Of The E.coli Holoenzyme/t7 Gp2 Complex" 80.37 522 100.00 100.00 6.32e-49 PDB 4LK1 "Crystal Structure Analysis Of The E.coli Holoenzyme" 80.37 613 100.00 100.00 9.23e-49 PDB 4LLG "Crystal Structure Analysis Of The E.coli Holoenzyme/gp2 Complex" 80.37 613 100.00 100.00 9.23e-49 PDB 4MEX "Crystal Structure Of Escherichia Coli Rna Polymerase In Complex With Salinamide A" 80.37 613 100.00 100.00 9.23e-49 PDB 4MEY "Crystal Structure Of Escherichia Coli Rna Polymerase Holoenzyme" 80.37 613 100.00 100.00 9.23e-49 PDB 4XSX "Crystal Structure Of Cbr 703 Bound To Escherichia Coli Rna Polymerase Holoenzyme" 80.37 522 100.00 100.00 6.32e-49 PDB 4XSY "Crystal Structure Of Cbr 9379 Bound To Escherichia Coli Rna Polymerase Holoenzyme" 80.37 522 100.00 100.00 6.32e-49 PDB 4XSZ "Crystal Structure Of Cbr 9393 Bound To Escherichia Coli Rna Polymerase Holoenzyme" 80.37 522 100.00 100.00 6.32e-49 PDB 4YFK "Escherichia Coli Rna Polymerase In Complex With Squaramide Compound 8." 80.37 613 100.00 100.00 9.23e-49 PDB 4YFN "Escherichia Coli Rna Polymerase In Complex With Squaramide Compound 14 (n-[3,4-dioxo-2-(4-{[4-(trifluoromethyl)benzyl]amino}pip" 80.37 613 100.00 100.00 9.23e-49 PDB 4YFX "Escherichia Coli Rna Polymerase In Complex With Myxopyronin B" 80.37 613 100.00 100.00 9.23e-49 PDB 4YG2 "X-ray Crystal Structur Of Escherichia Coli Rna Polymerase Sigma70 Holoenzyme" 80.37 613 100.00 100.00 9.23e-49 PDB 4YLN "E. Coli Transcription Initiation Complex - 17-bp Spacer And 4-nt Rna" 80.37 628 100.00 100.00 1.74e-48 PDB 4YLO "E. Coli Transcription Initiation Complex - 16-bp Spacer And 4-nt Rna" 80.37 628 100.00 100.00 1.74e-48 PDB 4YLP "E. Coli Transcription Initiation Complex - 16-bp Spacer And 5-nt Rna" 80.37 628 100.00 100.00 1.74e-48 PDB 4ZH2 "Crystal Structure Of Escherichia Coli Rna Polymerase In Complex With Cbr703" 80.37 613 100.00 100.00 9.23e-49 PDB 4ZH3 "Crystal Structure Of Escherichia Coli Rna Polymerase In Complex With Cbrh16-br" 80.37 613 100.00 100.00 9.23e-49 PDB 4ZH4 "Crystal Structure Of Escherichia Coli Rna Polymerase In Complex With Cbrp18" 80.37 613 100.00 100.00 9.23e-49 DBJ BAB37373 "RNA polymerase sigma 70 factor RpoD [Escherichia coli O157:H7 str. Sakai]" 80.37 613 98.84 100.00 1.39e-47 DBJ BAE77118 "RNA polymerase, sigma 70 (sigma D) factor [Escherichia coli str. K12 substr. W3110]" 80.37 613 100.00 100.00 9.23e-49 DBJ BAG66772 "RNA polymerase, sigma(70) factor [Escherichia coli O111:H-]" 80.37 613 98.84 100.00 1.39e-47 DBJ BAG78873 "RNA polymerase sigma factor RpoD [Escherichia coli SE11]" 80.37 613 98.84 100.00 1.39e-47 DBJ BAH65247 "RNA polymerase sigma factor [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]" 80.37 632 98.84 100.00 1.17e-47 EMBL CAD07736 "RNA polymerase sigma-70 factor [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" 80.37 660 98.84 100.00 1.54e-47 EMBL CAL34114 "RNA polymerase sigma factor rpoD [Cronobacter sakazakii]" 80.37 359 98.84 100.00 3.38e-49 EMBL CAP77541 "RNA polymerase sigma factor rpoD [Escherichia coli LF82]" 80.37 613 98.84 100.00 1.29e-47 EMBL CAQ33404 "RNA polymerase, sigma 70 (sigma D) factor, subunit of RNA polymerase sigma 70 [Escherichia coli BL21(DE3)]" 80.37 613 98.84 100.00 1.38e-47 EMBL CAQ90501 "RNA polymerase, sigma 70 (sigma D) factor [Escherichia fergusonii ATCC 35469]" 80.37 613 98.84 100.00 1.38e-47 GB AAA24601 "RNA polymerase sigma-subunit [Escherichia coli]" 80.37 613 100.00 100.00 8.69e-49 GB AAA27242 "rpoD protein [Salmonella enterica subsp. enterica serovar Typhimurium]" 80.37 615 98.84 100.00 1.48e-47 GB AAA89147 "CG Site No. 231; alternate gene name alt [Escherichia coli str. K-12 substr. MG1655]" 80.37 613 100.00 100.00 9.23e-49 GB AAB60181 "RNA polymerase sigma-subunit [Escherichia coli]" 80.37 603 98.84 100.00 1.29e-47 GB AAC76103 "RNA polymerase, sigma 70 (sigma D) factor [Escherichia coli str. K-12 substr. MG1655]" 80.37 613 100.00 100.00 9.23e-49 PIR AB0893 "RNA polymerase sigma-70 factor [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)" 80.37 660 98.84 100.00 1.54e-47 REF NP_311977 "RNA polymerase sigma factor RpoD [Escherichia coli O157:H7 str. Sakai]" 80.37 613 98.84 100.00 1.39e-47 REF NP_417539 "RNA polymerase, sigma 70 (sigma D) factor [Escherichia coli str. K-12 substr. MG1655]" 80.37 613 100.00 100.00 9.23e-49 REF NP_457602 "RNA polymerase sigma-70 factor [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" 80.37 660 98.84 100.00 1.54e-47 REF NP_462126 "RNA polymerase sigma factor RpoD [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]" 80.37 615 98.84 100.00 1.48e-47 REF NP_708877 "RNA polymerase sigma factor RpoD [Shigella flexneri 2a str. 301]" 80.37 613 98.84 100.00 1.39e-47 SP P00579 "RecName: Full=RNA polymerase sigma factor RpoD; AltName: Full=Sigma-70" 80.37 613 100.00 100.00 9.23e-49 SP P0A2E3 "RecName: Full=RNA polymerase sigma factor RpoD; AltName: Full=Sigma-70" 80.37 615 98.84 100.00 1.48e-47 SP P0A2E4 "RecName: Full=RNA polymerase sigma factor RpoD; AltName: Full=Sigma-70" 80.37 615 98.84 100.00 1.48e-47 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Gene_mnemonic $4_sigma70 'E. coli' 562 Eubacteria . Escherichia coli BL21(DE3) rpoD stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $4_sigma70 'recombinant technology' . Escherichia coli 'Escherichia coli BL21(DE3)' pET-15b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'no TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '0,3% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 0.3 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '2% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 2 % . stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '4% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 4 % . stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details '6% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 6 % . stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details '10% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 10 % . stop_ save_ save_sample_7 _Saveframe_category sample _Sample_type solution _Details '12% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 12 % . stop_ save_ save_sample_8 _Saveframe_category sample _Sample_type solution _Details '15% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM 'natural abundance' TFE 15 % . stop_ save_ save_sample_9 _Saveframe_category sample _Sample_type solution _Details '20% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 20 % . stop_ save_ save_sample_10 _Saveframe_category sample _Sample_type solution _Details '30% TFE (2,2,2-trifluoroehtanol)' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $4_sigma70 3 mM '[U-13C; U-15N]' TFE 30 % . stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 1.7 loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version 3.111 loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UnityPlus _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_4 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_7 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_8 save_ save_2D_1H-15N_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_9 save_ save_2D_1H-15N_HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_10 save_ ####################### # Sample conditions # ####################### save_sample_conditions_all _Saveframe_category sample_conditions _Details 'various concentrations of TFE (2,2,2-trifluoroehtanol)' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.0 . M pH 4.55 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER H H 8.344 0.02 1 2 3 3 SER N N 115.101 0.2 1 3 4 4 SER H H 8.299 0.02 1 4 4 4 SER N N 116.919 0.2 1 5 6 6 HIS H H 8.525 0.02 1 6 6 6 HIS N N 119.965 0.2 1 7 7 7 HIS H H 8.331 0.02 1 8 7 7 HIS N N 118.149 0.2 1 9 8 8 HIS H H 8.664 0.02 1 10 8 8 HIS N N 119.987 0.2 1 11 9 9 HIS H H 8.735 0.02 1 12 9 9 HIS N N 121.102 0.2 1 13 10 10 HIS H H 8.707 0.02 1 14 10 10 HIS N N 121.802 0.2 1 15 11 11 SER H H 8.517 0.02 1 16 11 11 SER N N 118.986 0.2 1 17 12 12 SER H H 8.546 0.02 1 18 12 12 SER N N 118.427 0.2 1 19 13 13 GLY H H 8.390 0.02 1 20 13 13 GLY N N 110.589 0.2 1 21 14 14 LEU H H 8.114 0.02 1 22 14 14 LEU N N 121.919 0.2 1 23 15 15 VAL H H 8.207 0.02 1 24 15 15 VAL N N 123.670 0.2 1 25 17 17 ARG H H 8.463 0.02 1 26 17 17 ARG N N 122.016 0.2 1 27 18 18 GLY H H 8.436 0.02 1 28 18 18 GLY N N 110.288 0.2 1 29 19 19 SER H H 8.177 0.02 1 30 19 19 SER N N 115.311 0.2 1 31 20 20 HIS H H 8.585 0.02 1 32 20 20 HIS N N 120.181 0.2 1 33 21 21 MET H H 8.335 0.02 1 34 21 21 MET N N 121.414 0.2 1 35 22 22 LEU H H 8.276 0.02 1 36 22 22 LEU N N 123.681 0.2 1 37 23 23 GLU H H 8.275 0.02 1 38 23 23 GLU N N 121.658 0.2 1 39 24 24 LEU H H 8.233 0.02 1 40 24 24 LEU N N 125.128 0.2 1 41 26 26 LEU H H 8.300 0.02 1 42 26 26 LEU N N 122.215 0.2 1 43 27 27 ASP H H 8.435 0.02 1 44 27 27 ASP N N 119.531 0.2 1 45 28 28 SER H H 8.225 0.02 1 46 28 28 SER N N 116.466 0.2 1 47 29 29 ALA H H 8.294 0.02 1 48 29 29 ALA N N 125.498 0.2 1 49 30 30 THR H H 8.087 0.02 1 50 30 30 THR N N 112.660 0.2 1 51 31 31 THR H H 8.062 0.02 1 52 31 31 THR N N 116.061 0.2 1 53 32 32 GLU H H 8.313 0.02 1 54 32 32 GLU N N 122.186 0.2 1 55 33 33 SER H H 8.253 0.02 1 56 33 33 SER N N 116.639 0.2 1 57 34 34 LEU H H 8.124 0.02 1 58 34 34 LEU N N 123.829 0.2 1 59 35 35 ARG H H 8.067 0.02 1 60 35 35 ARG N N 121.114 0.2 1 61 36 36 ALA H H 8.130 0.02 1 62 36 36 ALA N N 124.466 0.2 1 63 37 37 ALA H H 8.170 0.02 1 64 37 37 ALA N N 122.806 0.2 1 65 38 38 THR H H 7.958 0.02 1 66 38 38 THR N N 112.339 0.2 1 67 39 39 HIS H H 8.416 0.02 1 68 39 39 HIS N N 119.940 0.2 1 69 40 40 ASP H H 8.473 0.02 1 70 40 40 ASP N N 120.536 0.2 1 71 41 41 VAL H H 8.150 0.02 1 72 41 41 VAL N N 121.179 0.2 1 73 42 42 LEU H H 8.189 0.02 1 74 42 42 LEU N N 124.627 0.2 1 75 43 43 ALA H H 8.084 0.02 1 76 43 43 ALA N N 124.263 0.2 1 77 44 44 GLY H H 8.263 0.02 1 78 44 44 GLY N N 107.590 0.2 1 79 45 45 LEU H H 7.972 0.02 1 80 45 45 LEU N N 121.590 0.2 1 81 46 46 THR H H 8.028 0.02 1 82 46 46 THR N N 113.796 0.2 1 83 47 47 ALA H H 8.335 0.02 1 84 47 47 ALA N N 124.969 0.2 1 85 48 48 ARG H H 8.186 0.02 1 86 48 48 ARG N N 118.707 0.2 1 87 49 49 GLU H H 7.996 0.02 1 88 49 49 GLU N N 119.675 0.2 1 89 50 50 ALA H H 8.305 0.02 1 90 50 50 ALA N N 123.422 0.2 1 91 51 51 LYS H H 7.966 0.02 1 92 51 51 LYS N N 119.335 0.2 1 93 52 52 VAL H H 7.843 0.02 1 94 52 52 VAL N N 120.180 0.2 1 95 53 53 LEU H H 8.087 0.02 1 96 53 53 LEU N N 123.565 0.2 1 97 54 54 ARG H H 8.112 0.02 1 98 54 54 ARG N N 119.763 0.2 1 99 55 55 MET H H 8.135 0.02 1 100 55 55 MET N N 120.668 0.2 1 101 56 56 ARG H H 8.057 0.02 1 102 56 56 ARG N N 120.992 0.2 1 103 57 57 PHE H H 8.155 0.02 1 104 57 57 PHE N N 120.164 0.2 1 105 58 58 GLY H H 8.167 0.02 1 106 58 58 GLY N N 110.014 0.2 1 107 59 59 ILE H H 7.882 0.02 1 108 59 59 ILE N N 119.212 0.2 1 109 60 60 ASP H H 8.517 0.02 1 110 60 60 ASP N N 122.096 0.2 1 111 61 61 MET H H 8.297 0.02 1 112 61 61 MET N N 120.867 0.2 1 113 62 62 ASN H H 8.346 0.02 1 114 62 62 ASN N N 119.127 0.2 1 115 63 63 THR H H 8.015 0.02 1 116 63 63 THR N N 113.901 0.2 1 117 64 64 ASP H H 8.336 0.02 1 118 64 64 ASP N N 120.850 0.2 1 119 65 65 TYR H H 8.033 0.02 1 120 65 65 TYR N N 120.864 0.2 1 121 66 66 THR H H 7.955 0.02 1 122 66 66 THR N N 114.903 0.2 1 123 67 67 LEU H H 8.008 0.02 1 124 67 67 LEU N N 123.081 0.2 1 125 68 68 GLU H H 8.108 0.02 1 126 68 68 GLU N N 120.412 0.2 1 127 69 69 GLU H H 8.125 0.02 1 128 69 69 GLU N N 120.533 0.2 1 129 70 70 VAL H H 8.024 0.02 1 130 70 70 VAL N N 120.367 0.2 1 131 71 71 GLY H H 8.379 0.02 1 132 71 71 GLY N N 111.499 0.2 1 133 72 72 LYS H H 8.105 0.02 1 134 72 72 LYS N N 120.799 0.2 1 135 73 73 GLN H H 8.332 0.02 1 136 73 73 GLN N N 119.990 0.2 1 137 75 75 ASP H H 8.268 0.02 1 138 75 75 ASP N N 120.654 0.2 1 139 76 76 VAL H H 8.089 0.02 1 140 76 76 VAL N N 120.663 0.2 1 141 77 77 THR H H 8.093 0.02 1 142 77 77 THR N N 117.041 0.2 1 143 78 78 ARG H H 8.144 0.02 1 144 78 78 ARG N N 122.155 0.2 1 145 79 79 GLU H H 8.138 0.02 1 146 79 79 GLU N N 119.545 0.2 1 147 80 80 ARG H H 8.228 0.02 1 148 80 80 ARG N N 121.609 0.2 1 149 81 81 ILE H H 8.039 0.02 1 150 81 81 ILE N N 121.221 0.2 1 151 82 82 ARG H H 8.219 0.02 1 152 82 82 ARG N N 123.581 0.2 1 153 83 83 GLN H H 8.242 0.02 1 154 83 83 GLN N N 121.261 0.2 1 155 84 84 ILE H H 8.191 0.02 1 156 84 84 ILE N N 122.410 0.2 1 157 85 85 GLU H H 8.359 0.02 1 158 85 85 GLU N N 123.227 0.2 1 159 86 86 ALA H H 8.236 0.02 1 160 86 86 ALA N N 124.542 0.2 1 161 87 87 LYS H H 8.085 0.02 1 162 87 87 LYS N N 119.727 0.2 1 163 88 88 ALA H H 8.048 0.02 1 164 88 88 ALA N N 123.804 0.2 1 165 89 89 LEU H H 8.014 0.02 1 166 89 89 LEU N N 120.962 0.2 1 167 90 90 ARG H H 8.134 0.02 1 168 90 90 ARG N N 121.068 0.2 1 169 91 91 LYS H H 8.109 0.02 1 170 91 91 LYS N N 121.824 0.2 1 171 92 92 LEU H H 8.079 0.02 1 172 92 92 LEU N N 123.110 0.2 1 173 93 93 ARG H H 8.247 0.02 1 174 93 93 ARG N N 121.376 0.2 1 175 94 94 HIS H H 8.485 0.02 1 176 94 94 HIS N N 119.819 0.2 1 177 96 96 SER H H 8.489 0.02 1 178 96 96 SER N N 116.972 0.2 1 179 97 97 ARG H H 8.492 0.02 1 180 97 97 ARG N N 122.927 0.2 1 181 98 98 SER H H 8.331 0.02 1 182 98 98 SER N N 116.347 0.2 1 183 99 99 GLU H H 8.277 0.02 1 184 99 99 GLU N N 122.440 0.2 1 185 100 100 VAL H H 8.094 0.02 1 186 100 100 VAL N N 121.335 0.2 1 187 101 101 LEU H H 8.226 0.02 1 188 101 101 LEU N N 125.552 0.2 1 189 102 102 ARG H H 8.216 0.02 1 190 102 102 ARG N N 121.662 0.2 1 191 103 103 SER H H 8.121 0.02 1 192 103 103 SER N N 116.149 0.2 1 193 104 104 PHE H H 8.149 0.02 1 194 104 104 PHE N N 121.755 0.2 1 195 105 105 LEU H H 7.996 0.02 1 196 105 105 LEU N N 122.220 0.2 1 197 106 106 ASP H H 8.238 0.02 1 198 106 106 ASP N N 119.143 0.2 1 199 107 107 ASP H H 7.931 0.02 1 200 107 107 ASP N N 122.619 0.2 1 stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 115.101 0.2 1 2 3 3 SER H H 8.344 0.02 1 3 4 4 SER N N 116.919 0.2 1 4 4 4 SER H H 8.299 0.02 1 5 6 6 HIS N N 119.965 0.2 1 6 6 6 HIS H H 8.525 0.02 1 7 7 7 HIS N N 118.149 0.2 1 8 7 7 HIS H H 8.331 0.02 1 9 8 8 HIS N N 119.987 0.2 1 10 8 8 HIS H H 8.664 0.02 1 11 9 9 HIS N N 121.102 0.2 1 12 9 9 HIS H H 8.735 0.02 1 13 10 10 HIS N N 121.802 0.2 1 14 10 10 HIS H H 8.707 0.02 1 15 11 11 SER N N 118.986 0.2 1 16 11 11 SER H H 8.517 0.02 1 17 12 12 SER N N 118.427 0.2 1 18 12 12 SER H H 8.546 0.02 1 19 13 13 GLY N N 110.589 0.2 1 20 13 13 GLY H H 8.390 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.647 0.2 1 24 15 15 VAL H H 8.202 0.02 1 25 17 17 ARG N N 122.016 0.2 1 26 17 17 ARG H H 8.463 0.02 1 27 18 18 GLY N N 110.288 0.2 1 28 18 18 GLY H H 8.436 0.02 1 29 19 19 SER N N 115.311 0.2 1 30 19 19 SER H H 8.177 0.02 1 31 20 20 HIS N N 120.181 0.2 1 32 20 20 HIS H H 8.585 0.02 1 33 21 21 MET N N 121.414 0.2 1 34 21 21 MET H H 8.335 0.02 1 35 22 22 LEU N N 123.681 0.2 1 36 22 22 LEU H H 8.276 0.02 1 37 23 23 GLU N N 121.658 0.2 1 38 23 23 GLU H H 8.275 0.02 1 39 24 24 LEU N N 125.128 0.2 1 40 24 24 LEU H H 8.233 0.02 1 41 26 26 LEU N N 122.212 0.2 1 42 26 26 LEU H H 8.296 0.02 1 43 27 27 ASP N N 119.522 0.2 1 44 27 27 ASP H H 8.433 0.02 1 45 28 28 SER N N 116.466 0.2 1 46 28 28 SER H H 8.225 0.02 1 47 29 29 ALA N N 125.498 0.2 1 48 29 29 ALA H H 8.294 0.02 1 49 30 30 THR N N 112.640 0.2 1 50 30 30 THR H H 8.086 0.02 1 51 31 31 THR N N 116.044 0.2 1 52 31 31 THR H H 8.062 0.02 1 53 32 32 GLU N N 122.186 0.2 1 54 32 32 GLU H H 8.313 0.02 1 55 33 33 SER N N 116.639 0.2 1 56 33 33 SER H H 8.253 0.02 1 57 34 34 LEU N N 123.806 0.2 1 58 34 34 LEU H H 8.123 0.02 1 59 35 35 ARG N N 121.022 0.2 1 60 35 35 ARG H H 8.059 0.02 1 61 36 36 ALA N N 124.443 0.2 1 62 36 36 ALA H H 8.127 0.02 1 63 37 37 ALA N N 122.771 0.2 1 64 37 37 ALA H H 8.167 0.02 1 65 38 38 THR N N 112.304 0.2 1 66 38 38 THR H H 7.956 0.02 1 67 39 39 HIS N N 119.926 0.2 1 68 39 39 HIS H H 8.415 0.02 1 69 40 40 ASP N N 120.524 0.2 1 70 40 40 ASP H H 8.471 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 124.569 0.2 1 74 42 42 LEU H H 8.186 0.02 1 75 43 43 ALA N N 124.234 0.2 1 76 43 43 ALA H H 8.080 0.02 1 77 44 44 GLY N N 107.590 0.2 1 78 44 44 GLY H H 8.263 0.02 1 79 45 45 LEU N N 121.578 0.2 1 80 45 45 LEU H H 7.967 0.02 1 81 46 46 THR N N 113.796 0.2 1 82 46 46 THR H H 8.028 0.02 1 83 47 47 ALA N N 124.949 0.2 1 84 47 47 ALA H H 8.336 0.02 1 85 48 48 ARG N N 118.667 0.2 1 86 48 48 ARG H H 8.184 0.02 1 87 49 49 GLU N N 119.635 0.2 1 88 49 49 GLU H H 7.991 0.02 1 89 50 50 ALA N N 123.382 0.2 1 90 50 50 ALA H H 8.306 0.02 1 91 51 51 LYS N N 119.300 0.2 1 92 51 51 LYS H H 7.961 0.02 1 93 52 52 VAL N N 120.145 0.2 1 94 52 52 VAL H H 7.839 0.02 1 95 53 53 LEU N N 123.496 0.2 1 96 53 53 LEU H H 8.085 0.02 1 97 54 54 ARG N N 119.740 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.905 0.2 1 102 56 56 ARG H H 8.048 0.02 1 103 57 57 PHE N N 120.118 0.2 1 104 57 57 PHE H H 8.152 0.02 1 105 58 58 GLY N N 109.991 0.2 1 106 58 58 GLY H H 8.164 0.02 1 107 59 59 ILE N N 119.195 0.2 1 108 59 59 ILE H H 7.879 0.02 1 109 60 60 ASP N N 122.090 0.2 1 110 60 60 ASP H H 8.515 0.02 1 111 61 61 MET N N 120.850 0.2 1 112 61 61 MET H H 8.294 0.02 1 113 62 62 ASN N N 119.127 0.2 1 114 62 62 ASN H H 8.346 0.02 1 115 63 63 THR N N 113.863 0.2 1 116 63 63 THR H H 8.014 0.02 1 117 64 64 ASP N N 120.850 0.2 1 118 64 64 ASP H H 8.336 0.02 1 119 65 65 TYR N N 120.679 0.2 1 120 65 65 TYR H H 8.024 0.02 1 121 66 66 THR N N 114.871 0.2 1 122 66 66 THR H H 7.956 0.02 1 123 67 67 LEU N N 123.055 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.338 0.2 1 130 70 70 VAL H H 8.023 0.02 1 131 71 71 GLY N N 111.470 0.2 1 132 71 71 GLY H H 8.378 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.964 0.2 1 136 73 73 GLN H H 8.330 0.02 1 137 75 75 ASP N N 120.642 0.2 1 138 75 75 ASP H H 8.265 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 117.012 0.2 1 142 77 77 THR H H 8.090 0.02 1 143 78 78 ARG N N 122.117 0.2 1 144 78 78 ARG H H 8.143 0.02 1 145 79 79 GLU N N 119.481 0.2 1 146 79 79 GLU H H 8.134 0.02 1 147 80 80 ARG N N 121.569 0.2 1 148 80 80 ARG H H 8.227 0.02 1 149 81 81 ILE N N 121.129 0.2 1 150 81 81 ILE H H 8.034 0.02 1 151 82 82 ARG N N 123.535 0.2 1 152 82 82 ARG H H 8.216 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 122.378 0.2 1 156 84 84 ILE H H 8.188 0.02 1 157 85 85 GLU N N 123.167 0.2 1 158 85 85 GLU H H 8.357 0.02 1 159 86 86 ALA N N 124.473 0.2 1 160 86 86 ALA H H 8.234 0.02 1 161 87 87 LYS N N 119.687 0.2 1 162 87 87 LYS H H 8.080 0.02 1 163 88 88 ALA N N 123.769 0.2 1 164 88 88 ALA H H 8.043 0.02 1 165 89 89 LEU N N 120.988 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 123.072 0.2 1 172 92 92 LEU H H 8.073 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 119.805 0.2 1 176 94 94 HIS H H 8.483 0.02 1 177 96 96 SER N N 116.960 0.2 1 178 96 96 SER H H 8.486 0.02 1 179 97 97 ARG N N 122.927 0.2 1 180 97 97 ARG H H 8.492 0.02 1 181 98 98 SER N N 116.315 0.2 1 182 98 98 SER H H 8.331 0.02 1 183 99 99 GLU N N 122.434 0.2 1 184 99 99 GLU H H 8.275 0.02 1 185 100 100 VAL N N 121.306 0.2 1 186 100 100 VAL H H 8.091 0.02 1 187 101 101 LEU N N 125.517 0.2 1 188 101 101 LEU H H 8.223 0.02 1 189 102 102 ARG N N 121.622 0.2 1 190 102 102 ARG H H 8.215 0.02 1 191 103 103 SER N N 116.132 0.2 1 192 103 103 SER H H 8.119 0.02 1 193 104 104 PHE N N 121.743 0.2 1 194 104 104 PHE H H 8.146 0.02 1 195 105 105 LEU N N 122.194 0.2 1 196 105 105 LEU H H 7.994 0.02 1 197 106 106 ASP N N 119.131 0.2 1 198 106 106 ASP H H 8.235 0.02 1 199 107 107 ASP N N 122.619 0.2 1 200 107 107 ASP H H 7.931 0.02 1 stop_ save_ save_assigned_chem_shift_list_3 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_3 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 115.087 0.2 1 2 3 3 SER H H 8.336 0.02 1 3 4 4 SER N N 116.884 0.2 1 4 4 4 SER H H 8.293 0.02 1 5 6 6 HIS N N 119.913 0.2 1 6 6 6 HIS H H 8.517 0.02 1 7 7 7 HIS N N 118.071 0.2 1 8 7 7 HIS H H 8.322 0.02 1 9 8 8 HIS N N 119.923 0.2 1 10 8 8 HIS H H 8.655 0.02 1 11 9 9 HIS N N 121.024 0.2 1 12 9 9 HIS H H 8.728 0.02 1 13 10 10 HIS N N 121.721 0.2 1 14 10 10 HIS H H 8.700 0.02 1 15 11 11 SER N N 118.908 0.2 1 16 11 11 SER H H 8.508 0.02 1 17 12 12 SER N N 118.384 0.2 1 18 12 12 SER H H 8.535 0.02 1 19 13 13 GLY N N 110.543 0.2 1 20 13 13 GLY H H 8.381 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.531 0.2 1 24 15 15 VAL H H 8.188 0.02 1 25 17 17 ARG N N 121.964 0.2 1 26 17 17 ARG H H 8.447 0.02 1 27 18 18 GLY N N 110.230 0.2 1 28 18 18 GLY H H 8.427 0.02 1 29 19 19 SER N N 115.268 0.2 1 30 19 19 SER H H 8.164 0.02 1 31 20 20 HIS N N 120.138 0.2 1 32 20 20 HIS H H 8.574 0.02 1 33 21 21 MET N N 121.316 0.2 1 34 21 21 MET H H 8.324 0.02 1 35 22 22 LEU N N 123.606 0.2 1 36 22 22 LEU H H 8.261 0.02 1 37 23 23 GLU N N 121.580 0.2 1 38 23 23 GLU H H 8.252 0.02 1 39 24 24 LEU N N 125.024 0.2 1 40 24 24 LEU H H 8.214 0.02 1 41 26 26 LEU N N 122.160 0.2 1 42 26 26 LEU H H 8.279 0.02 1 43 27 27 ASP N N 119.436 0.2 1 44 27 27 ASP H H 8.416 0.02 1 45 28 28 SER N N 116.402 0.2 1 46 28 28 SER H H 8.215 0.02 1 47 29 29 ALA N N 125.428 0.2 1 48 29 29 ALA H H 8.288 0.02 1 49 30 30 THR N N 112.579 0.2 1 50 30 30 THR H H 8.075 0.02 1 51 31 31 THR N N 115.986 0.2 1 52 31 31 THR H H 8.049 0.02 1 53 32 32 GLU N N 122.053 0.2 1 54 32 32 GLU H H 8.304 0.02 1 55 33 33 SER N N 116.547 0.2 1 56 33 33 SER H H 8.234 0.02 1 57 34 34 LEU N N 123.757 0.2 1 58 34 34 LEU H H 8.106 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 124.239 0.2 1 62 36 36 ALA H H 8.106 0.02 1 63 37 37 ALA N N 122.595 0.2 1 64 37 37 ALA H H 8.144 0.02 1 65 38 38 THR N N 112.143 0.2 1 66 38 38 THR H H 7.935 0.02 1 67 39 39 HIS N N 119.822 0.2 1 68 39 39 HIS H H 8.385 0.02 1 69 40 40 ASP N N 120.345 0.2 1 70 40 40 ASP H H 8.451 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 124.159 0.2 1 74 42 42 LEU H H 8.154 0.02 1 75 43 43 ALA N N 124.015 0.2 1 76 43 43 ALA H H 8.048 0.02 1 77 44 44 GLY N N 107.463 0.2 1 78 44 44 GLY H H 8.242 0.02 1 79 45 45 LEU N N 121.561 0.2 1 80 45 45 LEU H H 7.931 0.02 1 81 46 46 THR N N 113.646 0.2 1 82 46 46 THR H H 8.013 0.02 1 83 47 47 ALA N N 124.725 0.2 1 84 47 47 ALA H H 8.356 0.02 1 85 48 48 ARG N N 118.476 0.2 1 86 48 48 ARG H H 8.176 0.02 1 87 49 49 GLU N N 119.415 0.2 1 88 49 49 GLU H H 7.952 0.02 1 89 50 50 ALA N N 122.954 0.2 1 90 50 50 ALA H H 8.317 0.02 1 91 51 51 LYS N N 119.046 0.2 1 92 51 51 LYS H H 7.914 0.02 1 93 52 52 VAL N N 119.880 0.2 1 94 52 52 VAL H H 7.800 0.02 1 95 53 53 LEU N N 122.889 0.2 1 96 53 53 LEU H H 8.065 0.02 1 97 54 54 ARG N N 119.512 0.2 1 98 54 54 ARG H H 8.100 0.02 1 99 55 55 MET N N 120.506 0.2 1 100 55 55 MET H H 8.113 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 119.852 0.2 1 104 57 57 PHE H H 8.129 0.02 1 105 58 58 GLY N N 109.832 0.2 1 106 58 58 GLY H H 8.122 0.02 1 107 59 59 ILE N N 119.125 0.2 1 108 59 59 ILE H H 7.855 0.02 1 109 60 60 ASP N N 122.035 0.2 1 110 60 60 ASP H H 8.496 0.02 1 111 61 61 MET N N 120.752 0.2 1 112 61 61 MET H H 8.280 0.02 1 113 62 62 ASN N N 119.006 0.2 1 114 62 62 ASN H H 8.326 0.02 1 115 63 63 THR N N 113.826 0.2 1 116 63 63 THR H H 7.998 0.02 1 117 64 64 ASP N N 120.789 0.2 1 118 64 64 ASP H H 8.325 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 114.672 0.2 1 122 66 66 THR H H 7.936 0.02 1 123 67 67 LEU N N 122.839 0.2 1 124 67 67 LEU H H 8.005 0.02 1 125 68 68 GLU N N 119.988 0.2 1 126 68 68 GLU H H 8.072 0.02 1 127 69 69 GLU N N 120.296 0.2 1 128 69 69 GLU H H 8.091 0.02 1 129 70 70 VAL N N 120.240 0.2 1 130 70 70 VAL H H 8.003 0.02 1 131 71 71 GLY N N 111.221 0.2 1 132 71 71 GLY H H 8.356 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.765 0.2 1 136 73 73 GLN H H 8.313 0.02 1 137 75 75 ASP N N 120.518 0.2 1 138 75 75 ASP H H 8.244 0.02 1 139 76 76 VAL N N 120.625 0.2 1 140 76 76 VAL H H 8.077 0.02 1 141 77 77 THR N N 116.876 0.2 1 142 77 77 THR H H 8.057 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 118.962 0.2 1 146 79 79 GLU H H 8.095 0.02 1 147 80 80 ARG N N 121.352 0.2 1 148 80 80 ARG H H 8.199 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 122.894 0.2 1 152 82 82 ARG H H 8.169 0.02 1 153 83 83 GLN N N 120.865 0.2 1 154 83 83 GLN H H 8.198 0.02 1 155 84 84 ILE N N 122.145 0.2 1 156 84 84 ILE H H 8.162 0.02 1 157 85 85 GLU N N 122.662 0.2 1 158 85 85 GLU H H 8.345 0.02 1 159 86 86 ALA N N 123.985 0.2 1 160 86 86 ALA H H 8.200 0.02 1 161 87 87 LYS N N 119.375 0.2 1 162 87 87 LYS H H 8.029 0.02 1 163 88 88 ALA N N 123.472 0.2 1 164 88 88 ALA H H 8.003 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 122.726 0.2 1 172 92 92 LEU H H 8.025 0.02 1 173 93 93 ARG N N 120.848 0.2 1 174 93 93 ARG H H 8.187 0.02 1 175 94 94 HIS N N 119.654 0.2 1 176 94 94 HIS H H 8.459 0.02 1 177 96 96 SER N N 116.888 0.2 1 178 96 96 SER H H 8.467 0.02 1 179 97 97 ARG N N 122.856 0.2 1 180 97 97 ARG H H 8.495 0.02 1 181 98 98 SER N N 116.119 0.2 1 182 98 98 SER H H 8.319 0.02 1 183 99 99 GLU N N 122.359 0.2 1 184 99 99 GLU H H 8.245 0.02 1 185 100 100 VAL N N 121.162 0.2 1 186 100 100 VAL H H 8.076 0.02 1 187 101 101 LEU N N 125.243 0.2 1 188 101 101 LEU H H 8.201 0.02 1 189 102 102 ARG N N 121.428 0.2 1 190 102 102 ARG H H 8.183 0.02 1 191 103 103 SER N N 115.990 0.2 1 192 103 103 SER H H 8.096 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 122.018 0.2 1 196 105 105 LEU H H 7.975 0.02 1 197 106 106 ASP N N 119.022 0.2 1 198 106 106 ASP H H 8.212 0.02 1 199 107 107 ASP N N 122.555 0.2 1 200 107 107 ASP H H 7.919 0.02 1 stop_ save_ save_assigned_chem_shift_list_4 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_4 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 115.058 0.2 1 2 3 3 SER H H 8.328 0.02 1 3 4 4 SER N N 116.850 0.2 1 4 4 4 SER H H 8.288 0.02 1 5 6 6 HIS N N 119.850 0.2 1 6 6 6 HIS H H 8.509 0.02 1 7 7 7 HIS N N 117.982 0.2 1 8 7 7 HIS H H 8.313 0.02 1 9 8 8 HIS N N 119.814 0.2 1 10 8 8 HIS H H 8.646 0.02 1 11 9 9 HIS N N 120.940 0.2 1 12 9 9 HIS H H 8.720 0.02 1 13 10 10 HIS N N 121.623 0.2 1 14 10 10 HIS H H 8.693 0.02 1 15 11 11 SER N N 118.830 0.2 1 16 11 11 SER H H 8.499 0.02 1 17 12 12 SER N N 118.329 0.2 1 18 12 12 SER H H 8.525 0.02 1 19 13 13 GLY N N 110.497 0.2 1 20 13 13 GLY H H 8.374 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.347 0.2 1 24 15 15 VAL H H 8.155 0.02 1 25 17 17 ARG N N 121.901 0.2 1 26 17 17 ARG H H 8.431 0.02 1 27 18 18 GLY N N 110.149 0.2 1 28 18 18 GLY H H 8.418 0.02 1 29 19 19 SER N N 115.198 0.2 1 30 19 19 SER H H 8.148 0.02 1 31 20 20 HIS N N 120.089 0.2 1 32 20 20 HIS H H 8.561 0.02 1 33 21 21 MET N N 121.255 0.2 1 34 21 21 MET H H 8.314 0.02 1 35 22 22 LEU N N 123.494 0.2 1 36 22 22 LEU H H 8.244 0.02 1 37 23 23 GLU N N 121.433 0.2 1 38 23 23 GLU H H 8.227 0.02 1 39 24 24 LEU N N 124.819 0.2 1 40 24 24 LEU H H 8.184 0.02 1 41 26 26 LEU N N 122.048 0.2 1 42 26 26 LEU H H 8.257 0.02 1 43 27 27 ASP N N 119.314 0.2 1 44 27 27 ASP H H 8.393 0.02 1 45 28 28 SER N N 116.365 0.2 1 46 28 28 SER H H 8.206 0.02 1 47 29 29 ALA N N 125.348 0.2 1 48 29 29 ALA H H 8.282 0.02 1 49 30 30 THR N N 112.513 0.2 1 50 30 30 THR H H 8.062 0.02 1 51 31 31 THR N N 115.928 0.2 1 52 31 31 THR H H 8.039 0.02 1 53 32 32 GLU N N 121.886 0.2 1 54 32 32 GLU H H 8.294 0.02 1 55 33 33 SER N N 116.397 0.2 1 56 33 33 SER H H 8.211 0.02 1 57 34 34 LEU N N 123.633 0.2 1 58 34 34 LEU H H 8.088 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 123.906 0.2 1 62 36 36 ALA H H 8.077 0.02 1 63 37 37 ALA N N 122.306 0.2 1 64 37 37 ALA H H 8.114 0.02 1 65 38 38 THR N N 111.851 0.2 1 66 38 38 THR H H 7.914 0.02 1 67 39 39 HIS N N 119.666 0.2 1 68 39 39 HIS H H 8.343 0.02 1 69 40 40 ASP N N 120.008 0.2 1 70 40 40 ASP H H 8.423 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 123.639 0.2 1 76 43 43 ALA H H 8.002 0.02 1 77 44 44 GLY N N 107.290 0.2 1 78 44 44 GLY H H 8.206 0.02 1 79 45 45 LEU N N 121.512 0.2 1 80 45 45 LEU H H 7.875 0.02 1 81 46 46 THR N N 113.426 0.2 1 82 46 46 THR H H 7.990 0.02 1 83 47 47 ALA N N 124.392 0.2 1 84 47 47 ALA H H 8.396 0.02 1 85 48 48 ARG N N 118.222 0.2 1 86 48 48 ARG H H 8.165 0.02 1 87 49 49 GLU N N 119.074 0.2 1 88 49 49 GLU H H 7.894 0.02 1 89 50 50 ALA N N 122.319 0.2 1 90 50 50 ALA H H 8.348 0.02 1 91 51 51 LYS N N 118.665 0.2 1 92 51 51 LYS H H 7.849 0.02 1 93 52 52 VAL N N 119.585 0.2 1 94 52 52 VAL H H 7.758 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 109.566 0.2 1 106 58 58 GLY H H 8.056 0.02 1 107 59 59 ILE N N 118.998 0.2 1 108 59 59 ILE H H 7.818 0.02 1 109 60 60 ASP N N 122.001 0.2 1 110 60 60 ASP H H 8.472 0.02 1 111 61 61 MET N N 120.604 0.2 1 112 61 61 MET H H 8.263 0.02 1 113 62 62 ASN N N 118.853 0.2 1 114 62 62 ASN H H 8.305 0.02 1 115 63 63 THR N N 113.768 0.2 1 116 63 63 THR H H 7.979 0.02 1 117 64 64 ASP N N 120.688 0.2 1 118 64 64 ASP H H 8.316 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 114.372 0.2 1 122 66 66 THR H H 7.913 0.02 1 123 67 67 LEU N N 122.509 0.2 1 124 67 67 LEU H H 8.011 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.610 0.2 1 130 70 70 VAL H H 8.014 0.02 1 131 71 71 GLY N N 110.742 0.2 1 132 71 71 GLY H H 8.325 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.436 0.2 1 136 73 73 GLN H H 8.283 0.02 1 137 75 75 ASP N N 120.258 0.2 1 138 75 75 ASP H H 8.219 0.02 1 139 76 76 VAL N N 120.576 0.2 1 140 76 76 VAL H H 8.065 0.02 1 141 77 77 THR N N 116.723 0.2 1 142 77 77 THR H H 8.005 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 118.038 0.2 1 146 79 79 GLU H H 8.032 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 122.290 0.2 1 152 82 82 ARG H H 8.114 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 121.804 0.2 1 156 84 84 ILE H H 8.134 0.02 1 157 85 85 GLU N N 121.850 0.2 1 158 85 85 GLU H H 8.345 0.02 1 159 86 86 ALA N N 123.330 0.2 1 160 86 86 ALA H H 8.162 0.02 1 161 87 87 LYS N N 118.890 0.2 1 162 87 87 LYS H H 7.948 0.02 1 163 88 88 ALA N N 123.033 0.2 1 164 88 88 ALA H H 7.954 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 122.200 0.2 1 172 92 92 LEU H H 7.955 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 119.388 0.2 1 176 94 94 HIS H H 8.431 0.02 1 177 96 96 SER N N 116.776 0.2 1 178 96 96 SER H H 8.435 0.02 1 179 97 97 ARG N N 122.777 0.2 1 180 97 97 ARG H H 8.508 0.02 1 181 98 98 SER N N 115.752 0.2 1 182 98 98 SER H H 8.305 0.02 1 183 99 99 GLU N N 122.249 0.2 1 184 99 99 GLU H H 8.201 0.02 1 185 100 100 VAL N N 120.991 0.2 1 186 100 100 VAL H H 8.053 0.02 1 187 101 101 LEU N N 124.726 0.2 1 188 101 101 LEU H H 8.175 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 115.745 0.2 1 192 103 103 SER H H 8.059 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 121.689 0.2 1 196 105 105 LEU H H 7.948 0.02 1 197 106 106 ASP N N 118.779 0.2 1 198 106 106 ASP H H 8.177 0.02 1 199 107 107 ASP N N 122.452 0.2 1 200 107 107 ASP H H 7.903 0.02 1 stop_ save_ save_assigned_chem_shift_list_5 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_5 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.948 0.2 1 2 3 3 SER H H 8.319 0.02 1 3 4 4 SER N N 116.786 0.2 1 4 4 4 SER H H 8.282 0.02 1 5 6 6 HIS N N 119.775 0.2 1 6 6 6 HIS H H 8.497 0.02 1 7 7 7 HIS N N 117.884 0.2 1 8 7 7 HIS H H 8.300 0.02 1 9 8 8 HIS N N 119.733 0.2 1 10 8 8 HIS H H 8.637 0.02 1 11 9 9 HIS N N 120.813 0.2 1 12 9 9 HIS H H 8.711 0.02 1 13 10 10 HIS N N 121.496 0.2 1 14 10 10 HIS H H 8.684 0.02 1 15 11 11 SER N N 118.720 0.2 1 16 11 11 SER H H 8.487 0.02 1 17 12 12 SER N N 118.265 0.2 1 18 12 12 SER H H 8.511 0.02 1 19 13 13 GLY N N 110.416 0.2 1 20 13 13 GLY H H 8.366 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.139 0.2 1 24 15 15 VAL H H 8.127 0.02 1 25 17 17 ARG N N 121.823 0.2 1 26 17 17 ARG H H 8.409 0.02 1 27 18 18 GLY N N 110.042 0.2 1 28 18 18 GLY H H 8.406 0.02 1 29 19 19 SER N N 115.106 0.2 1 30 19 19 SER H H 8.129 0.02 1 31 20 20 HIS N N 119.996 0.2 1 32 20 20 HIS H H 8.547 0.02 1 33 21 21 MET N N 121.085 0.2 1 34 21 21 MET H H 8.299 0.02 1 35 22 22 LEU N N 123.332 0.2 1 36 22 22 LEU H H 8.218 0.02 1 37 23 23 GLU N N 121.254 0.2 1 38 23 23 GLU H H 8.190 0.02 1 39 24 24 LEU N N 124.585 0.2 1 40 24 24 LEU H H 8.149 0.02 1 41 26 26 LEU N N 121.889 0.2 1 42 26 26 LEU H H 8.215 0.02 1 43 27 27 ASP N N 119.135 0.2 1 44 27 27 ASP H H 8.356 0.02 1 45 28 28 SER N N 116.258 0.2 1 46 28 28 SER H H 8.202 0.02 1 47 29 29 ALA N N 125.230 0.2 1 48 29 29 ALA H H 8.277 0.02 1 49 30 30 THR N N 112.513 0.2 1 50 30 30 THR H H 8.051 0.02 1 51 31 31 THR N N 115.836 0.2 1 52 31 31 THR H H 8.033 0.02 1 53 32 32 GLU N N 121.597 0.2 1 54 32 32 GLU H H 8.280 0.02 1 55 33 33 SER N N 116.267 0.2 1 56 33 33 SER H H 8.182 0.02 1 57 34 34 LEU N N 123.520 0.2 1 58 34 34 LEU H H 8.071 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 123.349 0.2 1 62 36 36 ALA H H 8.039 0.02 1 63 37 37 ALA N N 121.698 0.2 1 64 37 37 ALA H H 8.065 0.02 1 65 38 38 THR N N 111.435 0.2 1 66 38 38 THR H H 7.900 0.02 1 67 39 39 HIS N N 119.412 0.2 1 68 39 39 HIS H H 8.271 0.02 1 69 40 40 ASP N N 119.341 0.2 1 70 40 40 ASP H H 8.376 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 122.981 0.2 1 76 43 43 ALA H H 7.949 0.02 1 77 44 44 GLY N N 106.920 0.2 1 78 44 44 GLY H H 8.133 0.02 1 79 45 45 LEU N N 121.532 0.2 1 80 45 45 LEU H H 7.795 0.02 1 81 46 46 THR N N 113.282 0.2 1 82 46 46 THR H H 7.965 0.02 1 83 47 47 ALA N N 123.965 0.2 1 84 47 47 ALA H H 8.451 0.02 1 85 48 48 ARG N N 117.835 0.2 1 86 48 48 ARG H H 8.154 0.02 1 87 49 49 GLU N N 118.595 0.2 1 88 49 49 GLU H H 7.826 0.02 1 89 50 50 ALA N N 121.407 0.2 1 90 50 50 ALA H H 8.398 0.02 1 91 51 51 LYS N N 118.206 0.2 1 92 51 51 LYS H H 7.772 0.02 1 93 52 52 VAL N N 119.331 0.2 1 94 52 52 VAL H H 7.724 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 109.136 0.2 1 106 58 58 GLY H H 7.957 0.02 1 107 59 59 ILE N N 118.894 0.2 1 108 59 59 ILE H H 7.766 0.02 1 109 60 60 ASP N N 122.018 0.2 1 110 60 60 ASP H H 8.435 0.02 1 111 61 61 MET N N 120.442 0.2 1 112 61 61 MET H H 8.245 0.02 1 113 62 62 ASN N N 118.587 0.2 1 114 62 62 ASN H H 8.266 0.02 1 115 63 63 THR N N 113.762 0.2 1 116 63 63 THR H H 7.962 0.02 1 117 64 64 ASP N N 120.526 0.2 1 118 64 64 ASP H H 8.311 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 113.945 0.2 1 122 66 66 THR H H 7.879 0.02 1 123 67 67 LEU N N 122.163 0.2 1 124 67 67 LEU H H 8.026 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 109.812 0.2 1 132 71 71 GLY H H 8.287 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 118.893 0.2 1 136 73 73 GLN H H 8.231 0.02 1 137 75 75 ASP N N 119.845 0.2 1 138 75 75 ASP H H 8.188 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 116.665 0.2 1 142 77 77 THR H H 7.935 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 116.687 0.2 1 146 79 79 GLU H H 7.959 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.085 0.2 1 150 81 81 ILE H H 8.032 0.02 1 151 82 82 ARG N N 121.072 0.2 1 152 82 82 ARG H H 7.999 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 121.417 0.2 1 156 84 84 ILE H H 8.107 0.02 1 157 85 85 GLU N N 120.649 0.2 1 158 85 85 GLU H H 8.382 0.02 1 159 86 86 ALA N N 122.085 0.2 1 160 86 86 ALA H H 8.110 0.02 1 161 87 87 LYS N N 118.157 0.2 1 162 87 87 LYS H H 7.833 0.02 1 163 88 88 ALA N N 122.450 0.2 1 164 88 88 ALA H H 7.926 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 121.256 0.2 1 172 92 92 LEU H H 7.853 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.892 0.2 1 176 94 94 HIS H H 8.383 0.02 1 177 96 96 SER N N 116.522 0.2 1 178 96 96 SER H H 8.373 0.02 1 179 97 97 ARG N N 122.659 0.2 1 180 97 97 ARG H H 8.542 0.02 1 181 98 98 SER N N 115.111 0.2 1 182 98 98 SER H H 8.288 0.02 1 183 99 99 GLU N N 122.090 0.2 1 184 99 99 GLU H H 8.110 0.02 1 185 100 100 VAL N N 120.662 0.2 1 186 100 100 VAL H H 8.026 0.02 1 187 101 101 LEU N N 123.767 0.2 1 188 101 101 LEU H H 8.142 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 115.320 0.2 1 192 103 103 SER H H 7.997 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 120.984 0.2 1 196 105 105 LEU H H 7.913 0.02 1 197 106 106 ASP N N 118.372 0.2 1 198 106 106 ASP H H 8.118 0.02 1 199 107 107 ASP N N 122.296 0.2 1 200 107 107 ASP H H 7.883 0.02 1 stop_ save_ save_assigned_chem_shift_list_6 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_6 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.966 0.2 1 2 3 3 SER H H 8.307 0.02 1 3 4 4 SER N N 116.760 0.2 1 4 4 4 SER H H 8.274 0.02 1 5 6 6 HIS N N 119.703 0.2 1 6 6 6 HIS H H 8.486 0.02 1 7 7 7 HIS N N 117.739 0.2 1 8 7 7 HIS H H 8.291 0.02 1 9 8 8 HIS N N 119.618 0.2 1 10 8 8 HIS H H 8.621 0.02 1 11 9 9 HIS N N 120.674 0.2 1 12 9 9 HIS H H 8.697 0.02 1 13 10 10 HIS N N 121.386 0.2 1 14 10 10 HIS H H 8.672 0.02 1 15 11 11 SER N N 118.616 0.2 1 16 11 11 SER H H 8.474 0.02 1 17 12 12 SER N N 118.197 0.2 1 18 12 12 SER H H 8.498 0.02 1 19 13 13 GLY N N 110.347 0.2 1 20 13 13 GLY H H 8.354 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.670 0.2 1 24 15 15 VAL H H 8.207 0.02 1 25 17 17 ARG N N 121.745 0.2 1 26 17 17 ARG H H 8.385 0.02 1 27 18 18 GLY N N 109.941 0.2 1 28 18 18 GLY H H 8.393 0.02 1 29 19 19 SER N N 115.013 0.2 1 30 19 19 SER H H 8.108 0.02 1 31 20 20 HIS N N 119.910 0.2 1 32 20 20 HIS H H 8.527 0.02 1 33 21 21 MET N N 120.920 0.2 1 34 21 21 MET H H 8.281 0.02 1 35 22 22 LEU N N 123.110 0.2 1 36 22 22 LEU H H 8.184 0.02 1 37 23 23 GLU N N 121.063 0.2 1 38 23 23 GLU H H 8.149 0.02 1 39 24 24 LEU N N 124.288 0.2 1 40 24 24 LEU H H 8.106 0.02 1 41 26 26 LEU N N 121.704 0.2 1 42 26 26 LEU H H 8.161 0.02 1 43 27 27 ASP N N 118.976 0.2 1 44 27 27 ASP H H 8.318 0.02 1 45 28 28 SER N N 116.200 0.2 1 46 28 28 SER H H 8.206 0.02 1 47 29 29 ALA N N 125.126 0.2 1 48 29 29 ALA H H 8.272 0.02 1 49 30 30 THR N N 112.628 0.2 1 50 30 30 THR H H 8.046 0.02 1 51 31 31 THR N N 115.781 0.2 1 52 31 31 THR H H 8.035 0.02 1 53 32 32 GLU N N 121.303 0.2 1 54 32 32 GLU H H 8.269 0.02 1 55 33 33 SER N N 116.157 0.2 1 56 33 33 SER H H 8.149 0.02 1 57 34 34 LEU N N 123.422 0.2 1 58 34 34 LEU H H 8.061 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 122.849 0.2 1 62 36 36 ALA H H 8.018 0.02 1 63 37 37 ALA N N 122.806 0.2 1 64 37 37 ALA H H 8.170 0.02 1 65 38 38 THR N N 111.164 0.2 1 66 38 38 THR H H 7.914 0.02 1 67 39 39 HIS N N 119.218 0.2 1 68 39 39 HIS H H 8.200 0.02 1 69 40 40 ASP N N 118.729 0.2 1 70 40 40 ASP H H 8.330 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 122.415 0.2 1 76 43 43 ALA H H 7.952 0.02 1 77 44 44 GLY N N 106.551 0.2 1 78 44 44 GLY H H 8.043 0.02 1 79 45 45 LEU N N 121.708 0.2 1 80 45 45 LEU H H 7.776 0.02 1 81 46 46 THR N N 113.256 0.2 1 82 46 46 THR H H 7.955 0.02 1 83 47 47 ALA N N 123.641 0.2 1 84 47 47 ALA H H 8.433 0.02 1 85 48 48 ARG N N 117.642 0.2 1 86 48 48 ARG H H 8.110 0.02 1 87 49 49 GLU N N 118.323 0.2 1 88 49 49 GLU H H 7.813 0.02 1 89 50 50 ALA N N 120.780 0.2 1 90 50 50 ALA H H 8.414 0.02 1 91 51 51 LYS N N 117.897 0.2 1 92 51 51 LYS H H 7.723 0.02 1 93 52 52 VAL N N 119.307 0.2 1 94 52 52 VAL H H 7.729 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 108.827 0.2 1 106 58 58 GLY H H 7.902 0.02 1 107 59 59 ILE N N 118.868 0.2 1 108 59 59 ILE H H 7.744 0.02 1 109 60 60 ASP N N 121.963 0.2 1 110 60 60 ASP H H 8.405 0.02 1 111 61 61 MET N N 120.324 0.2 1 112 61 61 MET H H 8.228 0.02 1 113 62 62 ASN N N 118.400 0.2 1 114 62 62 ASN H H 8.234 0.02 1 115 63 63 THR N N 113.964 0.2 1 116 63 63 THR H H 7.962 0.02 1 117 64 64 ASP N N 120.393 0.2 1 118 64 64 ASP H H 8.306 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 113.795 0.2 1 122 66 66 THR H H 7.856 0.02 1 123 67 67 LEU N N 123.084 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 108.834 0.2 1 132 71 71 GLY H H 8.258 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 118.307 0.2 1 136 73 73 GLN H H 8.197 0.02 1 137 75 75 ASP N N 119.496 0.2 1 138 75 75 ASP H H 8.177 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 116.804 0.2 1 142 77 77 THR H H 7.895 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 115.982 0.2 1 146 79 79 GLU H H 7.937 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 123.581 0.2 1 152 82 82 ARG H H 8.219 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 121.238 0.2 1 156 84 84 ILE H H 8.076 0.02 1 157 85 85 GLU N N 119.910 0.2 1 158 85 85 GLU H H 8.444 0.02 1 159 86 86 ALA N N 122.920 0.2 1 160 86 86 ALA H H 8.091 0.02 1 161 87 87 LYS N N 117.683 0.2 1 162 87 87 LYS H H 7.759 0.02 1 163 88 88 ALA N N 122.161 0.2 1 164 88 88 ALA H H 7.954 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 120.460 0.2 1 172 92 92 LEU H H 7.781 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.409 0.2 1 176 94 94 HIS H H 8.338 0.02 1 177 96 96 SER N N 116.256 0.2 1 178 96 96 SER H H 8.305 0.02 1 179 97 97 ARG N N 122.589 0.2 1 180 97 97 ARG H H 8.574 0.02 1 181 98 98 SER N N 114.470 0.2 1 182 98 98 SER H H 8.271 0.02 1 183 99 99 GLU N N 121.908 0.2 1 184 99 99 GLU H H 8.025 0.02 1 185 100 100 VAL N N 120.535 0.2 1 186 100 100 VAL H H 7.992 0.02 1 187 101 101 LEU N N 124.281 0.2 1 188 101 101 LEU H H 8.106 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 114.928 0.2 1 192 103 103 SER H H 7.936 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 120.337 0.2 1 196 105 105 LEU H H 7.883 0.02 1 197 106 106 ASP N N 117.976 0.2 1 198 106 106 ASP H H 8.059 0.02 1 199 107 107 ASP N N 122.157 0.2 1 200 107 107 ASP H H 7.867 0.02 1 stop_ save_ save_assigned_chem_shift_list_7 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_7 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.943 0.2 1 2 3 3 SER H H 8.303 0.02 1 3 4 4 SER N N 116.697 0.2 1 4 4 4 SER H H 8.272 0.02 1 5 6 6 HIS N N 119.619 0.2 1 6 6 6 HIS H H 8.484 0.02 1 7 7 7 HIS N N 117.670 0.2 1 8 7 7 HIS H H 8.281 0.02 1 9 8 8 HIS N N 119.525 0.2 1 10 8 8 HIS H H 8.614 0.02 1 11 9 9 HIS N N 120.559 0.2 1 12 9 9 HIS H H 8.693 0.02 1 13 10 10 HIS N N 121.276 0.2 1 14 10 10 HIS H H 8.667 0.02 1 15 11 11 SER N N 118.501 0.2 1 16 11 11 SER H H 8.468 0.02 1 17 12 12 SER N N 118.135 0.2 1 18 12 12 SER H H 8.487 0.02 1 19 13 13 GLY N N 110.278 0.2 1 20 13 13 GLY H H 8.347 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.670 0.2 1 24 15 15 VAL H H 8.207 0.02 1 25 17 17 ARG N N 121.664 0.2 1 26 17 17 ARG H H 8.364 0.02 1 27 18 18 GLY N N 109.837 0.2 1 28 18 18 GLY H H 8.385 0.02 1 29 19 19 SER N N 114.927 0.2 1 30 19 19 SER H H 8.093 0.02 1 31 20 20 HIS N N 119.823 0.2 1 32 20 20 HIS H H 8.514 0.02 1 33 21 21 MET N N 120.732 0.2 1 34 21 21 MET H H 8.264 0.02 1 35 22 22 LEU N N 122.864 0.2 1 36 22 22 LEU H H 8.147 0.02 1 37 23 23 GLU N N 120.861 0.2 1 38 23 23 GLU H H 8.109 0.02 1 39 24 24 LEU N N 123.947 0.2 1 40 24 24 LEU H H 8.058 0.02 1 41 26 26 LEU N N 121.476 0.2 1 42 26 26 LEU H H 8.099 0.02 1 43 27 27 ASP N N 118.817 0.2 1 44 27 27 ASP H H 8.287 0.02 1 45 28 28 SER N N 116.166 0.2 1 46 28 28 SER H H 8.216 0.02 1 47 29 29 ALA N N 124.999 0.2 1 48 29 29 ALA H H 8.263 0.02 1 49 30 30 THR N N 112.747 0.2 1 50 30 30 THR H H 8.052 0.02 1 51 31 31 THR N N 115.714 0.2 1 52 31 31 THR H H 8.041 0.02 1 53 32 32 GLU N N 121.092 0.2 1 54 32 32 GLU H H 8.266 0.02 1 55 33 33 SER N N 116.082 0.2 1 56 33 33 SER H H 8.131 0.02 1 57 34 34 LEU N N 123.358 0.2 1 58 34 34 LEU H H 8.066 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 122.491 0.2 1 62 36 36 ALA H H 8.018 0.02 1 63 37 37 ALA N N 122.806 0.2 1 64 37 37 ALA H H 8.170 0.02 1 65 38 38 THR N N 111.066 0.2 1 66 38 38 THR H H 7.951 0.02 1 67 39 39 HIS N N 119.311 0.2 1 68 39 39 HIS H H 8.196 0.02 1 69 40 40 ASP N N 118.304 0.2 1 70 40 40 ASP H H 8.223 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 123.639 0.2 1 76 43 43 ALA H H 8.002 0.02 1 77 44 44 GLY N N 106.320 0.2 1 78 44 44 GLY H H 7.989 0.02 1 79 45 45 LEU N N 122.020 0.2 1 80 45 45 LEU H H 7.854 0.02 1 81 46 46 THR N N 113.368 0.2 1 82 46 46 THR H H 7.973 0.02 1 83 47 47 ALA N N 123.480 0.2 1 84 47 47 ALA H H 8.341 0.02 1 85 48 48 ARG N N 118.222 0.2 1 86 48 48 ARG H H 8.165 0.02 1 87 49 49 GLU N N 117.919 0.2 1 88 49 49 GLU H H 7.658 0.02 1 89 50 50 ALA N N 120.494 0.2 1 90 50 50 ALA H H 8.391 0.02 1 91 51 51 LYS N N 117.712 0.2 1 92 51 51 LYS H H 7.699 0.02 1 93 52 52 VAL N N 119.340 0.2 1 94 52 52 VAL H H 7.765 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 108.622 0.2 1 106 58 58 GLY H H 7.904 0.02 1 107 59 59 ILE N N 118.369 0.2 1 108 59 59 ILE H H 7.700 0.02 1 109 60 60 ASP N N 121.864 0.2 1 110 60 60 ASP H H 8.385 0.02 1 111 61 61 MET N N 120.185 0.2 1 112 61 61 MET H H 8.227 0.02 1 113 62 62 ASN N N 118.192 0.2 1 114 62 62 ASN H H 8.235 0.02 1 115 63 63 THR N N 114.392 0.2 1 116 63 63 THR H H 7.999 0.02 1 117 64 64 ASP N N 120.304 0.2 1 118 64 64 ASP H H 8.306 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 113.996 0.2 1 122 66 66 THR H H 7.862 0.02 1 123 67 67 LEU N N 123.084 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 108.143 0.2 1 132 71 71 GLY H H 8.244 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 117.935 0.2 1 136 73 73 GLN H H 8.059 0.02 1 137 75 75 ASP N N 119.086 0.2 1 138 75 75 ASP H H 8.165 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 116.986 0.2 1 142 77 77 THR H H 7.909 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 115.942 0.2 1 146 79 79 GLU H H 7.970 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 123.581 0.2 1 152 82 82 ARG H H 8.219 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 122.410 0.2 1 156 84 84 ILE H H 8.191 0.02 1 157 85 85 GLU N N 119.540 0.2 1 158 85 85 GLU H H 8.494 0.02 1 159 86 86 ALA N N 122.628 0.2 1 160 86 86 ALA H H 8.055 0.02 1 161 87 87 LYS N N 117.487 0.2 1 162 87 87 LYS H H 7.734 0.02 1 163 88 88 ALA N N 122.115 0.2 1 164 88 88 ALA H H 8.025 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 119.977 0.2 1 172 92 92 LEU H H 7.753 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.216 0.2 1 176 94 94 HIS H H 8.302 0.02 1 177 96 96 SER N N 116.071 0.2 1 178 96 96 SER H H 8.263 0.02 1 179 97 97 ARG N N 122.563 0.2 1 180 97 97 ARG H H 8.596 0.02 1 181 98 98 SER N N 113.996 0.2 1 182 98 98 SER H H 8.260 0.02 1 183 99 99 GLU N N 121.770 0.2 1 184 99 99 GLU H H 7.971 0.02 1 185 100 100 VAL N N 120.394 0.2 1 186 100 100 VAL H H 7.968 0.02 1 187 101 101 LEU N N 123.944 0.2 1 188 101 101 LEU H H 8.058 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 114.668 0.2 1 192 103 103 SER H H 7.899 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 119.933 0.2 1 196 105 105 LEU H H 7.879 0.02 1 197 106 106 ASP N N 117.705 0.2 1 198 106 106 ASP H H 8.035 0.02 1 199 107 107 ASP N N 122.028 0.2 1 200 107 107 ASP H H 7.858 0.02 1 stop_ save_ save_assigned_chem_shift_list_8 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_8 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.885 0.2 1 2 3 3 SER H H 8.296 0.02 1 3 4 4 SER N N 116.642 0.2 1 4 4 4 SER H H 8.267 0.02 1 5 6 6 HIS N N 119.541 0.2 1 6 6 6 HIS H H 8.471 0.02 1 7 7 7 HIS N N 117.552 0.2 1 8 7 7 HIS H H 8.275 0.02 1 9 8 8 HIS N N 119.415 0.2 1 10 8 8 HIS H H 8.606 0.02 1 11 9 9 HIS N N 120.363 0.2 1 12 9 9 HIS H H 8.677 0.02 1 13 10 10 HIS N N 121.123 0.2 1 14 10 10 HIS H H 8.660 0.02 1 15 11 11 SER N N 118.362 0.2 1 16 11 11 SER H H 8.456 0.02 1 17 12 12 SER N N 118.043 0.2 1 18 12 12 SER H H 8.471 0.02 1 19 13 13 GLY N N 110.157 0.2 1 20 13 13 GLY H H 8.334 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.670 0.2 1 24 15 15 VAL H H 8.207 0.02 1 25 17 17 ARG N N 121.531 0.2 1 26 17 17 ARG H H 8.326 0.02 1 27 18 18 GLY N N 109.699 0.2 1 28 18 18 GLY H H 8.372 0.02 1 29 19 19 SER N N 114.840 0.2 1 30 19 19 SER H H 8.069 0.02 1 31 20 20 HIS N N 119.650 0.2 1 32 20 20 HIS H H 8.487 0.02 1 33 21 21 MET N N 120.438 0.2 1 34 21 21 MET H H 8.237 0.02 1 35 22 22 LEU N N 122.466 0.2 1 36 22 22 LEU H H 8.083 0.02 1 37 23 23 GLU N N 120.535 0.2 1 38 23 23 GLU H H 8.056 0.02 1 39 24 24 LEU N N 123.468 0.2 1 40 24 24 LEU H H 7.984 0.02 1 41 26 26 LEU N N 121.115 0.2 1 42 26 26 LEU H H 8.001 0.02 1 43 27 27 ASP N N 118.650 0.2 1 44 27 27 ASP H H 8.257 0.02 1 45 28 28 SER N N 116.137 0.2 1 46 28 28 SER H H 8.232 0.02 1 47 29 29 ALA N N 124.849 0.2 1 48 29 29 ALA H H 8.251 0.02 1 49 30 30 THR N N 112.923 0.2 1 50 30 30 THR H H 8.061 0.02 1 51 31 31 THR N N 115.587 0.2 1 52 31 31 THR H H 8.035 0.02 1 53 32 32 GLU N N 120.832 0.2 1 54 32 32 GLU H H 8.262 0.02 1 55 33 33 SER N N 115.984 0.2 1 56 33 33 SER H H 8.115 0.02 1 57 34 34 LEU N N 123.327 0.2 1 58 34 34 LEU H H 8.075 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 122.430 0.2 1 62 36 36 ALA H H 8.032 0.02 1 63 37 37 ALA N N 122.806 0.2 1 64 37 37 ALA H H 8.170 0.02 1 65 38 38 THR N N 111.193 0.2 1 66 38 38 THR H H 8.019 0.02 1 67 39 39 HIS N N 118.979 0.2 1 68 39 39 HIS H H 8.148 0.02 1 69 40 40 ASP N N 117.600 0.2 1 70 40 40 ASP H H 8.148 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 123.639 0.2 1 76 43 43 ALA H H 8.002 0.02 1 77 44 44 GLY N N 106.274 0.2 1 78 44 44 GLY H H 7.954 0.02 1 79 45 45 LEU N N 122.199 0.2 1 80 45 45 LEU H H 7.999 0.02 1 81 46 46 THR N N 113.640 0.2 1 82 46 46 THR H H 8.054 0.02 1 83 47 47 ALA N N 123.370 0.2 1 84 47 47 ALA H H 8.149 0.02 1 85 48 48 ARG N N 118.222 0.2 1 86 48 48 ARG H H 8.165 0.02 1 87 49 49 GLU N N 117.573 0.2 1 88 49 49 GLU H H 7.645 0.02 1 89 50 50 ALA N N 120.269 0.2 1 90 50 50 ALA H H 8.342 0.02 1 91 51 51 LYS N N 117.611 0.2 1 92 51 51 LYS H H 7.675 0.02 1 93 52 52 VAL N N 119.573 0.2 1 94 52 52 VAL H H 7.822 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 108.183 0.2 1 106 58 58 GLY H H 7.947 0.02 1 107 59 59 ILE N N 118.103 0.2 1 108 59 59 ILE H H 7.677 0.02 1 109 60 60 ASP N N 121.767 0.2 1 110 60 60 ASP H H 8.374 0.02 1 111 61 61 MET N N 120.038 0.2 1 112 61 61 MET H H 8.253 0.02 1 113 62 62 ASN N N 118.079 0.2 1 114 62 62 ASN H H 8.252 0.02 1 115 63 63 THR N N 115.408 0.2 1 116 63 63 THR H H 8.096 0.02 1 117 64 64 ASP N N 120.223 0.2 1 118 64 64 ASP H H 8.290 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 114.632 0.2 1 122 66 66 THR H H 7.905 0.02 1 123 67 67 LEU N N 123.084 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 107.508 0.2 1 132 71 71 GLY H H 8.223 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.410 0.2 1 136 73 73 GLN H H 8.284 0.02 1 137 75 75 ASP N N 120.258 0.2 1 138 75 75 ASP H H 8.219 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 117.142 0.2 1 142 77 77 THR H H 7.964 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 118.038 0.2 1 146 79 79 GLU H H 8.032 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 123.581 0.2 1 152 82 82 ARG H H 8.219 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 122.410 0.2 1 156 84 84 ILE H H 8.191 0.02 1 157 85 85 GLU N N 119.355 0.2 1 158 85 85 GLU H H 8.558 0.02 1 159 86 86 ALA N N 123.370 0.2 1 160 86 86 ALA H H 8.168 0.02 1 161 87 87 LYS N N 117.409 0.2 1 162 87 87 LYS H H 7.727 0.02 1 163 88 88 ALA N N 122.193 0.2 1 164 88 88 ALA H H 7.999 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 119.607 0.2 1 172 92 92 LEU H H 7.749 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.095 0.2 1 176 94 94 HIS H H 8.275 0.02 1 177 96 96 SER N N 115.933 0.2 1 178 96 96 SER H H 8.225 0.02 1 179 97 97 ARG N N 122.517 0.2 1 180 97 97 ARG H H 8.629 0.02 1 181 98 98 SER N N 113.442 0.2 1 182 98 98 SER H H 8.246 0.02 1 183 99 99 GLU N N 121.576 0.2 1 184 99 99 GLU H H 7.890 0.02 1 185 100 100 VAL N N 120.316 0.2 1 186 100 100 VAL H H 7.943 0.02 1 187 101 101 LEU N N 123.470 0.2 1 188 101 101 LEU H H 7.984 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 114.425 0.2 1 192 103 103 SER H H 7.859 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 119.552 0.2 1 196 105 105 LEU H H 7.881 0.02 1 197 106 106 ASP N N 117.405 0.2 1 198 106 106 ASP H H 8.007 0.02 1 199 107 107 ASP N N 121.924 0.2 1 200 107 107 ASP H H 7.836 0.02 1 stop_ save_ save_assigned_chem_shift_list_9 _Saveframe_category assigned_chemical_shifts _Details 'M1 is deleted in the expression system.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_9 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.839 0.2 1 2 3 3 SER H H 8.293 0.02 1 3 4 4 SER N N 116.593 0.2 1 4 4 4 SER H H 8.266 0.02 1 5 6 6 HIS N N 119.307 0.2 1 6 6 6 HIS H H 8.438 0.02 1 7 7 7 HIS N N 117.413 0.2 1 8 7 7 HIS H H 8.268 0.02 1 9 8 8 HIS N N 119.392 0.2 1 10 8 8 HIS H H 8.598 0.02 1 11 9 9 HIS N N 120.120 0.2 1 12 9 9 HIS H H 8.674 0.02 1 13 10 10 HIS N N 120.956 0.2 1 14 10 10 HIS H H 8.655 0.02 1 15 11 11 SER N N 118.247 0.2 1 16 11 11 SER H H 8.445 0.02 1 17 12 12 SER N N 117.930 0.2 1 18 12 12 SER H H 8.454 0.02 1 19 13 13 GLY N N 110.021 0.2 1 20 13 13 GLY H H 8.319 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.670 0.2 1 24 15 15 VAL H H 8.207 0.02 1 25 17 17 ARG N N 121.361 0.2 1 26 17 17 ARG H H 8.280 0.02 1 27 18 18 GLY N N 109.522 0.2 1 28 18 18 GLY H H 8.357 0.02 1 29 19 19 SER N N 114.730 0.2 1 30 19 19 SER H H 8.048 0.02 1 31 20 20 HIS N N 119.500 0.2 1 32 20 20 HIS H H 8.463 0.02 1 33 21 21 MET N N 120.083 0.2 1 34 21 21 MET H H 8.208 0.02 1 35 22 22 LEU N N 121.986 0.2 1 36 22 22 LEU H H 8.013 0.02 1 37 23 23 GLU N N 120.145 0.2 1 38 23 23 GLU H H 7.998 0.02 1 39 24 24 LEU N N 124.857 0.2 1 40 24 24 LEU H H 8.190 0.02 1 41 26 26 LEU N N 120.850 0.2 1 42 26 26 LEU H H 8.143 0.02 1 43 27 27 ASP N N 118.589 0.2 1 44 27 27 ASP H H 8.242 0.02 1 45 28 28 SER N N 116.224 0.2 1 46 28 28 SER H H 8.265 0.02 1 47 29 29 ALA N N 124.719 0.2 1 48 29 29 ALA H H 8.242 0.02 1 49 30 30 THR N N 113.194 0.2 1 50 30 30 THR H H 8.087 0.02 1 51 31 31 THR N N 115.535 0.2 1 52 31 31 THR H H 8.028 0.02 1 53 32 32 GLU N N 120.699 0.2 1 54 32 32 GLU H H 8.252 0.02 1 55 33 33 SER N N 115.917 0.2 1 56 33 33 SER H H 8.103 0.02 1 57 34 34 LEU N N 123.266 0.2 1 58 34 34 LEU H H 8.099 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 122.246 0.2 1 62 36 36 ALA H H 8.065 0.02 1 63 37 37 ALA N N 122.806 0.2 1 64 37 37 ALA H H 8.170 0.02 1 65 38 38 THR N N 111.447 0.2 1 66 38 38 THR H H 8.111 0.02 1 67 39 39 HIS N N 118.898 0.2 1 68 39 39 HIS H H 8.150 0.02 1 69 40 40 ASP N N 120.008 0.2 1 70 40 40 ASP H H 8.423 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 123.639 0.2 1 76 43 43 ALA H H 8.002 0.02 1 77 44 44 GLY N N 106.389 0.2 1 78 44 44 GLY H H 7.967 0.02 1 79 45 45 LEU N N 121.708 0.2 1 80 45 45 LEU H H 7.935 0.02 1 81 46 46 THR N N 114.067 0.2 1 82 46 46 THR H H 8.182 0.02 1 83 47 47 ALA N N 123.248 0.2 1 84 47 47 ALA H H 7.957 0.02 1 85 48 48 ARG N N 118.222 0.2 1 86 48 48 ARG H H 8.165 0.02 1 87 49 49 GLU N N 117.399 0.2 1 88 49 49 GLU H H 7.649 0.02 1 89 50 50 ALA N N 120.148 0.2 1 90 50 50 ALA H H 8.297 0.02 1 91 51 51 LYS N N 117.510 0.2 1 92 51 51 LYS H H 7.660 0.02 1 93 52 52 VAL N N 119.695 0.2 1 94 52 52 VAL H H 7.898 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 107.461 0.2 1 106 58 58 GLY H H 8.049 0.02 1 107 59 59 ILE N N 118.039 0.2 1 108 59 59 ILE H H 7.684 0.02 1 109 60 60 ASP N N 121.770 0.2 1 110 60 60 ASP H H 8.398 0.02 1 111 61 61 MET N N 119.666 0.2 1 112 61 61 MET H H 8.331 0.02 1 113 62 62 ASN N N 118.853 0.2 1 114 62 62 ASN H H 8.305 0.02 1 115 63 63 THR N N 116.009 0.2 1 116 63 63 THR H H 8.108 0.02 1 117 64 64 ASP N N 120.445 0.2 1 118 64 64 ASP H H 8.361 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 115.388 0.2 1 122 66 66 THR H H 7.982 0.02 1 123 67 67 LEU N N 123.084 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 107.061 0.2 1 132 71 71 GLY H H 8.223 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.410 0.2 1 136 73 73 GLN H H 8.284 0.02 1 137 75 75 ASP N N 120.258 0.2 1 138 75 75 ASP H H 8.219 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 117.332 0.2 1 142 77 77 THR H H 8.019 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 118.038 0.2 1 146 79 79 GLU H H 8.032 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 123.581 0.2 1 152 82 82 ARG H H 8.219 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 122.410 0.2 1 156 84 84 ILE H H 8.191 0.02 1 157 85 85 GLU N N 119.223 0.2 1 158 85 85 GLU H H 8.601 0.02 1 159 86 86 ALA N N 123.370 0.2 1 160 86 86 ALA H H 8.168 0.02 1 161 87 87 LYS N N 117.420 0.2 1 162 87 87 LYS H H 7.731 0.02 1 163 88 88 ALA N N 121.976 0.2 1 164 88 88 ALA H H 8.004 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 119.376 0.2 1 172 92 92 LEU H H 7.775 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.193 0.2 1 176 94 94 HIS H H 8.199 0.02 1 177 96 96 SER N N 115.887 0.2 1 178 96 96 SER H H 8.208 0.02 1 179 97 97 ARG N N 122.433 0.2 1 180 97 97 ARG H H 8.676 0.02 1 181 98 98 SER N N 112.876 0.2 1 182 98 98 SER H H 8.237 0.02 1 183 99 99 GLU N N 121.415 0.2 1 184 99 99 GLU H H 7.800 0.02 1 185 100 100 VAL N N 120.350 0.2 1 186 100 100 VAL H H 7.934 0.02 1 187 101 101 LEU N N 122.918 0.2 1 188 101 101 LEU H H 7.908 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 114.246 0.2 1 192 103 103 SER H H 7.831 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 119.200 0.2 1 196 105 105 LEU H H 7.896 0.02 1 197 106 106 ASP N N 117.070 0.2 1 198 106 106 ASP H H 7.981 0.02 1 199 107 107 ASP N N 121.846 0.2 1 200 107 107 ASP H H 7.820 0.02 1 stop_ save_ save_assigned_chem_shift_list_10 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_10 stop_ _Sample_conditions_label $sample_conditions_all _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name '4 sigma70' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 SER N N 114.832 0.2 1 2 3 3 SER H H 8.289 0.02 1 3 4 4 SER N N 116.581 0.2 1 4 4 4 SER H H 8.268 0.02 1 5 6 6 HIS N N 119.200 0.2 1 6 6 6 HIS H H 8.421 0.02 1 7 7 7 HIS N N 117.376 0.2 1 8 7 7 HIS H H 8.264 0.02 1 9 8 8 HIS N N 119.399 0.2 1 10 8 8 HIS H H 8.588 0.02 1 11 9 9 HIS N N 120.016 0.2 1 12 9 9 HIS H H 8.668 0.02 1 13 10 10 HIS N N 120.850 0.2 1 14 10 10 HIS H H 8.650 0.02 1 15 11 11 SER N N 118.131 0.2 1 16 11 11 SER H H 8.436 0.02 1 17 12 12 SER N N 117.878 0.2 1 18 12 12 SER H H 8.444 0.02 1 19 13 13 GLY N N 109.931 0.2 1 20 13 13 GLY H H 8.305 0.02 1 21 14 14 LEU N N 121.919 0.2 1 22 14 14 LEU H H 8.114 0.02 1 23 15 15 VAL N N 123.670 0.2 1 24 15 15 VAL H H 8.207 0.02 1 25 17 17 ARG N N 121.214 0.2 1 26 17 17 ARG H H 8.243 0.02 1 27 18 18 GLY N N 109.349 0.2 1 28 18 18 GLY H H 8.341 0.02 1 29 19 19 SER N N 114.626 0.2 1 30 19 19 SER H H 8.030 0.02 1 31 20 20 HIS N N 119.402 0.2 1 32 20 20 HIS H H 8.446 0.02 1 33 21 21 MET N N 119.855 0.2 1 34 21 21 MET H H 8.188 0.02 1 35 22 22 LEU N N 121.571 0.2 1 36 22 22 LEU H H 7.952 0.02 1 37 23 23 GLU N N 119.824 0.2 1 38 23 23 GLU H H 7.952 0.02 1 39 24 24 LEU N N 124.857 0.2 1 40 24 24 LEU H H 8.190 0.02 1 41 26 26 LEU N N 120.521 0.2 1 42 26 26 LEU H H 8.164 0.02 1 43 27 27 ASP N N 118.679 0.2 1 44 27 27 ASP H H 8.261 0.02 1 45 28 28 SER N N 116.359 0.2 1 46 28 28 SER H H 8.291 0.02 1 47 29 29 ALA N N 124.586 0.2 1 48 29 29 ALA H H 8.243 0.02 1 49 30 30 THR N N 113.400 0.2 1 50 30 30 THR H H 8.116 0.02 1 51 31 31 THR N N 115.561 0.2 1 52 31 31 THR H H 8.018 0.02 1 53 32 32 GLU N N 120.399 0.2 1 54 32 32 GLU H H 8.238 0.02 1 55 33 33 SER N N 115.888 0.2 1 56 33 33 SER H H 8.104 0.02 1 57 34 34 LEU N N 123.185 0.2 1 58 34 34 LEU H H 8.110 0.02 1 59 35 35 ARG N N 121.114 0.2 1 60 35 35 ARG H H 8.067 0.02 1 61 36 36 ALA N N 122.269 0.2 1 62 36 36 ALA H H 8.097 0.02 1 63 37 37 ALA N N 122.806 0.2 1 64 37 37 ALA H H 8.170 0.02 1 65 38 38 THR N N 111.528 0.2 1 66 38 38 THR H H 8.144 0.02 1 67 39 39 HIS N N 118.829 0.2 1 68 39 39 HIS H H 8.145 0.02 1 69 40 40 ASP N N 120.008 0.2 1 70 40 40 ASP H H 8.423 0.02 1 71 41 41 VAL N N 121.179 0.2 1 72 41 41 VAL H H 8.150 0.02 1 73 42 42 LEU N N 123.449 0.2 1 74 42 42 LEU H H 8.106 0.02 1 75 43 43 ALA N N 123.639 0.2 1 76 43 43 ALA H H 8.002 0.02 1 77 44 44 GLY N N 106.424 0.2 1 78 44 44 GLY H H 7.985 0.02 1 79 45 45 LEU N N 121.587 0.2 1 80 45 45 LEU H H 7.948 0.02 1 81 46 46 THR N N 114.217 0.2 1 82 46 46 THR H H 8.224 0.02 1 83 47 47 ALA N N 123.277 0.2 1 84 47 47 ALA H H 7.928 0.02 1 85 48 48 ARG N N 118.222 0.2 1 86 48 48 ARG H H 8.165 0.02 1 87 49 49 GLU N N 117.336 0.2 1 88 49 49 GLU H H 7.657 0.02 1 89 50 50 ALA N N 120.252 0.2 1 90 50 50 ALA H H 8.252 0.02 1 91 51 51 LYS N N 117.427 0.2 1 92 51 51 LYS H H 7.665 0.02 1 93 52 52 VAL N N 120.116 0.2 1 94 52 52 VAL H H 7.896 0.02 1 95 53 53 LEU N N 123.565 0.2 1 96 53 53 LEU H H 8.087 0.02 1 97 54 54 ARG N N 119.763 0.2 1 98 54 54 ARG H H 8.112 0.02 1 99 55 55 MET N N 120.668 0.2 1 100 55 55 MET H H 8.135 0.02 1 101 56 56 ARG N N 120.992 0.2 1 102 56 56 ARG H H 8.057 0.02 1 103 57 57 PHE N N 120.164 0.2 1 104 57 57 PHE H H 8.155 0.02 1 105 58 58 GLY N N 107.115 0.2 1 106 58 58 GLY H H 8.118 0.02 1 107 59 59 ILE N N 118.034 0.2 1 108 59 59 ILE H H 7.696 0.02 1 109 60 60 ASP N N 121.923 0.2 1 110 60 60 ASP H H 8.428 0.02 1 111 61 61 MET N N 120.604 0.2 1 112 61 61 MET H H 8.263 0.02 1 113 62 62 ASN N N 118.853 0.2 1 114 62 62 ASN H H 8.305 0.02 1 115 63 63 THR N N 116.690 0.2 1 116 63 63 THR H H 8.153 0.02 1 117 64 64 ASP N N 120.636 0.2 1 118 64 64 ASP H H 8.451 0.02 1 119 65 65 TYR N N 120.864 0.2 1 120 65 65 TYR H H 8.033 0.02 1 121 66 66 THR N N 116.093 0.2 1 122 66 66 THR H H 8.017 0.02 1 123 67 67 LEU N N 123.084 0.2 1 124 67 67 LEU H H 8.008 0.02 1 125 68 68 GLU N N 120.412 0.2 1 126 68 68 GLU H H 8.108 0.02 1 127 69 69 GLU N N 120.533 0.2 1 128 69 69 GLU H H 8.125 0.02 1 129 70 70 VAL N N 120.367 0.2 1 130 70 70 VAL H H 8.024 0.02 1 131 71 71 GLY N N 106.896 0.2 1 132 71 71 GLY H H 8.234 0.02 1 133 72 72 LYS N N 120.799 0.2 1 134 72 72 LYS H H 8.105 0.02 1 135 73 73 GLN N N 119.410 0.2 1 136 73 73 GLN H H 8.284 0.02 1 137 75 75 ASP N N 120.258 0.2 1 138 75 75 ASP H H 8.219 0.02 1 139 76 76 VAL N N 120.663 0.2 1 140 76 76 VAL H H 8.089 0.02 1 141 77 77 THR N N 117.220 0.2 1 142 77 77 THR H H 8.072 0.02 1 143 78 78 ARG N N 122.155 0.2 1 144 78 78 ARG H H 8.144 0.02 1 145 79 79 GLU N N 118.038 0.2 1 146 79 79 GLU H H 8.032 0.02 1 147 80 80 ARG N N 121.609 0.2 1 148 80 80 ARG H H 8.228 0.02 1 149 81 81 ILE N N 121.221 0.2 1 150 81 81 ILE H H 8.039 0.02 1 151 82 82 ARG N N 123.581 0.2 1 152 82 82 ARG H H 8.219 0.02 1 153 83 83 GLN N N 121.261 0.2 1 154 83 83 GLN H H 8.242 0.02 1 155 84 84 ILE N N 122.410 0.2 1 156 84 84 ILE H H 8.191 0.02 1 157 85 85 GLU N N 119.142 0.2 1 158 85 85 GLU H H 8.618 0.02 1 159 86 86 ALA N N 123.370 0.2 1 160 86 86 ALA H H 8.168 0.02 1 161 87 87 LYS N N 117.438 0.2 1 162 87 87 LYS H H 7.743 0.02 1 163 88 88 ALA N N 121.234 0.2 1 164 88 88 ALA H H 7.953 0.02 1 165 89 89 LEU N N 120.962 0.2 1 166 89 89 LEU H H 8.014 0.02 1 167 90 90 ARG N N 121.068 0.2 1 168 90 90 ARG H H 8.134 0.02 1 169 91 91 LYS N N 121.824 0.2 1 170 91 91 LYS H H 8.109 0.02 1 171 92 92 LEU N N 119.249 0.2 1 172 92 92 LEU H H 7.798 0.02 1 173 93 93 ARG N N 121.376 0.2 1 174 93 93 ARG H H 8.247 0.02 1 175 94 94 HIS N N 118.314 0.2 1 176 94 94 HIS H H 8.161 0.02 1 177 96 96 SER N N 115.898 0.2 1 178 96 96 SER H H 8.205 0.02 1 179 97 97 ARG N N 122.402 0.2 1 180 97 97 ARG H H 8.709 0.02 1 181 98 98 SER N N 112.547 0.2 1 182 98 98 SER H H 8.230 0.02 1 183 99 99 GLU N N 121.319 0.2 1 184 99 99 GLU H H 7.745 0.02 1 185 100 100 VAL N N 120.423 0.2 1 186 100 100 VAL H H 7.932 0.02 1 187 101 101 LEU N N 122.491 0.2 1 188 101 101 LEU H H 7.872 0.02 1 189 102 102 ARG N N 121.662 0.2 1 190 102 102 ARG H H 8.216 0.02 1 191 103 103 SER N N 114.183 0.2 1 192 103 103 SER H H 7.821 0.02 1 193 104 104 PHE N N 121.755 0.2 1 194 104 104 PHE H H 8.149 0.02 1 195 105 105 LEU N N 118.957 0.2 1 196 105 105 LEU H H 7.911 0.02 1 197 106 106 ASP N N 116.850 0.2 1 198 106 106 ASP H H 7.968 0.02 1 199 107 107 ASP N N 121.814 0.2 1 200 107 107 ASP H H 7.798 0.02 1 stop_ save_