data_16323 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; SLBP RBD 30-mer peptide ; _BMRB_accession_number 16323 _BMRB_flat_file_name bmr16323.str _Entry_type original _Submission_date 2009-05-28 _Accession_date 2009-05-28 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details '30 residue peptide from the SLBP RNA binding domain sequence' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Thapar Roopa . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 155 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-05-16 update BMRB 'update entry citation' 2012-08-03 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 22907757 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Krishnan Nithya . . 2 Lam Tukiet T. . 3 Fritz Andrew . . 4 Rempinski Donald . . 5 Minderman Kieran . . 6 Berezney Hans . . 7 Marzluff Ronald . . 8 Thapar William F. . stop_ _Journal_abbreviation 'Mol. Cell. Biol.' _Journal_name_full 'Molecular and cellular biology' _Journal_volume 32 _Journal_issue 21 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4306 _Page_last 4322 _Year 2012 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name SLBP30mer _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SLBP30mer $SLBP30mer stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'Binds histone mRNAs' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SLBP30mer _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SLBP30mer _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'RNA binding protein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 30 _Mol_residue_sequence ; ETDESVLMRRQKQINYGKNT IAYDRYIKEV ; loop_ _Residue_seq_code _Residue_label 1 GLU 2 THR 3 ASP 4 GLU 5 SER 6 VAL 7 LEU 8 MET 9 ARG 10 ARG 11 GLN 12 LYS 13 GLN 14 ILE 15 ASN 16 TYR 17 GLY 18 LYS 19 ASN 20 THR 21 ILE 22 ALA 23 TYR 24 ASP 25 ARG 26 TYR 27 ILE 28 LYS 29 GLU 30 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2KJM "Solution Structure Of Slbp Rna Binding Domain Fragment" 100.00 30 100.00 100.00 1.66e-11 PDB 4L8R "Structure Of Mrna Stem-loop, Human Stem-loop Binding Protein And 3'hexo Ternary Complex" 100.00 120 100.00 100.00 2.21e-11 PDB 4QOZ "Crystal Structure Of The Histone Mrna Stem-loop, Stem-loop Binding Protein (phosphorylated), And 3'hexo Ternary Complex" 100.00 120 100.00 100.00 2.21e-11 DBJ BAE20786 "unnamed protein product [Mus musculus]" 100.00 250 100.00 100.00 4.42e-09 DBJ BAE22851 "unnamed protein product [Mus musculus]" 93.33 195 100.00 100.00 3.61e-10 DBJ BAE32343 "unnamed protein product [Mus musculus]" 100.00 275 100.00 100.00 2.87e-09 DBJ BAE36737 "unnamed protein product [Mus musculus]" 100.00 275 100.00 100.00 2.87e-09 DBJ BAG38003 "unnamed protein product [Homo sapiens]" 100.00 270 100.00 100.00 1.31e-09 EMBL CAA94918 "histone RNA hairpin-binding protein [Homo sapiens]" 100.00 270 100.00 100.00 1.31e-09 GB AAB97091 "histone stem-loop binding protein [Homo sapiens]" 100.00 270 100.00 100.00 1.31e-09 GB AAC53530 "histone stem-loop binding protein [Mus musculus]" 100.00 275 100.00 100.00 2.87e-09 GB AAH14908 "Stem-loop binding protein [Homo sapiens]" 100.00 270 100.00 100.00 1.31e-09 GB AAH15703 "Stem-loop binding protein [Homo sapiens]" 100.00 270 100.00 100.00 1.31e-09 GB AAH29631 "Slbp protein [Mus musculus]" 100.00 255 100.00 100.00 1.07e-09 REF NP_001039345 "histone RNA hairpin-binding protein [Bos taurus]" 100.00 271 100.00 100.00 8.04e-10 REF NP_001231786 "histone RNA hairpin-binding protein [Sus scrofa]" 100.00 275 100.00 100.00 7.84e-11 REF NP_001233401 "histone RNA hairpin-binding protein [Pan troglodytes]" 100.00 270 100.00 100.00 2.33e-09 REF NP_001276653 "histone RNA hairpin-binding protein isoform 2 [Mus musculus]" 100.00 255 100.00 100.00 1.07e-09 REF NP_001276654 "histone RNA hairpin-binding protein isoform 3 [Mus musculus]" 100.00 240 100.00 100.00 1.81e-09 SP P97440 "RecName: Full=Histone RNA hairpin-binding protein; AltName: Full=Histone stem-loop-binding protein" 100.00 275 100.00 100.00 2.87e-09 SP Q14493 "RecName: Full=Histone RNA hairpin-binding protein; AltName: Full=Histone stem-loop-binding protein" 100.00 270 100.00 100.00 1.31e-09 TPG DAA28416 "TPA: histone stem loop binding protein [Bos taurus]" 100.00 272 100.00 100.00 7.73e-10 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $SLBP30mer Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $SLBP30mer 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $SLBP30mer . mM 1 7 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' NaCl 50 mM . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version . loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 700 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_DQF-COSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D DQF-COSY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.050 . M pH 5 . pH pressure 1 . atm temperature 278 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D DQF-COSY' '2D 1H-1H NOESY' '2D 1H-1H TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name SLBP30mer _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 GLU H H 8.55 0.02 1 2 1 1 GLU HA H 4.48 0.02 1 3 1 1 GLU HB2 H 2.64 0.02 1 4 1 1 GLU HB3 H 2.64 0.02 1 5 1 1 GLU HG2 H 2.69 0.02 1 6 1 1 GLU HG3 H 2.69 0.02 1 7 2 2 THR H H 8.71 0.02 1 8 2 2 THR HA H 4.18 0.02 1 9 2 2 THR HB H 4.01 0.02 1 10 2 2 THR HG2 H 1.04 0.02 1 11 3 3 ASP H H 8.62 0.02 1 12 3 3 ASP HA H 4.48 0.02 1 13 3 3 ASP HB2 H 2.69 0.02 1 14 3 3 ASP HB3 H 2.64 0.02 1 15 4 4 GLU H H 8.47 0.02 1 16 4 4 GLU HA H 4.11 0.02 1 17 4 4 GLU HB2 H 1.92 0.02 1 18 4 4 GLU HB3 H 1.82 0.02 1 19 4 4 GLU HG2 H 2.30 0.02 1 20 4 4 GLU HG3 H 2.30 0.02 1 21 5 5 SER H H 8.27 0.02 1 22 5 5 SER HA H 4.14 0.02 1 23 5 5 SER HB2 H 3.80 0.02 1 24 5 5 SER HB3 H 3.74 0.02 1 25 6 6 VAL H H 7.95 0.02 1 26 6 6 VAL HA H 3.95 0.02 1 27 6 6 VAL HB H 1.94 0.02 1 28 6 6 VAL HG1 H 0.72 0.02 1 29 6 6 VAL HG2 H 0.72 0.02 1 30 7 7 LEU H H 7.93 0.02 1 31 7 7 LEU HA H 4.00 0.02 1 32 7 7 LEU HB2 H 1.68 0.02 1 33 7 7 LEU HB3 H 1.68 0.02 1 34 7 7 LEU HG H 0.98 0.02 1 35 8 8 MET H H 8.17 0.02 1 36 8 8 MET HA H 4.34 0.02 1 37 8 8 MET HB2 H 2.58 0.02 1 38 8 8 MET HB3 H 2.56 0.02 1 39 8 8 MET HG2 H 2.65 0.02 1 40 8 8 MET HG3 H 2.63 0.02 1 41 9 9 ARG H H 7.91 0.02 1 42 9 9 ARG HA H 3.95 0.02 1 43 9 9 ARG HB2 H 1.49 0.02 1 44 9 9 ARG HB3 H 1.49 0.02 1 45 9 9 ARG HG2 H 1.22 0.02 1 46 9 9 ARG HG3 H 1.22 0.02 1 47 10 10 ARG H H 8.04 0.02 1 48 10 10 ARG HA H 4.17 0.02 1 49 10 10 ARG HB2 H 1.69 0.02 1 50 10 10 ARG HB3 H 1.55 0.02 1 51 10 10 ARG HG2 H 1.46 0.02 1 52 11 11 GLN H H 8.33 0.02 1 53 11 11 GLN HA H 4.58 0.02 1 54 11 11 GLN HB2 H 2.60 0.02 1 55 11 11 GLN HB3 H 2.58 0.02 1 56 11 11 GLN HE21 H 7.52 0.02 1 57 11 11 GLN HE22 H 6.81 0.02 1 58 11 11 GLN HG2 H 2.70 0.02 1 59 11 11 GLN HG3 H 2.67 0.02 1 60 12 12 LYS H H 7.93 0.02 1 61 12 12 LYS HA H 4.31 0.02 1 62 12 12 LYS HB2 H 1.68 0.02 1 63 12 12 LYS HB3 H 1.53 0.02 1 64 12 12 LYS HE2 H 3.02 0.02 1 65 12 12 LYS HG2 H 1.43 0.02 1 66 13 13 GLN H H 8.23 0.02 1 67 13 13 GLN HA H 4.20 0.02 1 68 13 13 GLN HB2 H 1.90 0.02 1 69 13 13 GLN HB3 H 1.78 0.02 1 70 13 13 GLN HE21 H 7.48 0.02 1 71 13 13 GLN HE22 H 6.82 0.02 1 72 13 13 GLN HG2 H 2.28 0.02 1 73 13 13 GLN HG3 H 2.28 0.02 1 74 14 14 ILE H H 8.09 0.02 1 75 14 14 ILE HA H 3.81 0.02 1 76 14 14 ILE HB H 1.60 0.02 1 77 14 14 ILE HG12 H 1.49 0.02 1 78 14 14 ILE HG13 H 1.24 0.02 1 79 15 15 ASN H H 8.29 0.02 1 80 15 15 ASN HA H 4.51 0.02 1 81 15 15 ASN HB2 H 2.60 0.02 1 82 15 15 ASN HB3 H 2.50 0.02 1 83 15 15 ASN HD21 H 7.38 0.02 1 84 15 15 ASN HD22 H 6.80 0.02 1 85 16 16 TYR H H 8.15 0.02 1 86 16 16 TYR HA H 4.28 0.02 1 87 16 16 TYR HB2 H 2.93 0.02 1 88 16 16 TYR HB3 H 2.74 0.02 1 89 17 17 GLY H H 8.24 0.02 1 90 17 17 GLY HA2 H 3.68 0.02 1 91 17 17 GLY HA3 H 3.66 0.02 1 92 18 18 LYS H H 8.10 0.02 1 93 18 18 LYS HA H 4.00 0.02 1 94 18 18 LYS HB2 H 1.63 0.02 1 95 18 18 LYS HB3 H 1.61 0.02 1 96 18 18 LYS HD2 H 1.54 0.02 1 97 18 18 LYS HG2 H 1.29 0.02 1 98 19 19 ASN H H 8.34 0.02 1 99 19 19 ASN HA H 4.60 0.02 1 100 19 19 ASN HB2 H 2.69 0.02 1 101 19 19 ASN HB3 H 2.60 0.02 1 102 19 19 ASN HD21 H 7.43 0.02 1 103 19 19 ASN HD22 H 6.77 0.02 1 104 20 20 THR H H 7.93 0.02 1 105 20 20 THR HA H 4.11 0.02 1 106 20 20 THR HB H 4.05 0.02 1 107 20 20 THR HG2 H 1.00 0.02 1 108 21 21 ILE H H 8.09 0.02 1 109 21 21 ILE HA H 3.85 0.02 1 110 21 21 ILE HB H 1.63 0.02 1 111 21 21 ILE HG12 H 1.54 0.02 1 112 21 21 ILE HG13 H 1.54 0.02 1 113 22 22 ALA H H 8.16 0.02 1 114 22 22 ALA HA H 4.03 0.02 1 115 22 22 ALA HB H 1.12 0.02 1 116 23 23 TYR H H 8.24 0.02 1 117 23 23 TYR HA H 4.35 0.02 1 118 23 23 TYR HB2 H 2.62 0.02 1 119 23 23 TYR HB3 H 2.58 0.02 1 120 24 24 ASP H H 7.93 0.02 1 121 24 24 ASP HA H 4.33 0.02 1 122 24 24 ASP HB2 H 2.80 0.02 1 123 24 24 ASP HB3 H 2.69 0.02 1 124 25 25 ARG H H 8.00 0.02 1 125 25 25 ARG HA H 4.02 0.02 1 126 25 25 ARG HB2 H 1.70 0.02 1 127 25 25 ARG HB3 H 1.62 0.02 1 128 25 25 ARG HG2 H 1.55 0.02 1 129 25 25 ARG HG3 H 1.46 0.02 1 130 26 26 TYR H H 7.93 0.02 1 131 26 26 TYR HA H 4.32 0.02 1 132 26 26 TYR HB2 H 2.85 0.02 1 133 26 26 TYR HB3 H 2.75 0.02 1 134 27 27 ILE H H 7.72 0.02 1 135 27 27 ILE HA H 3.82 0.02 1 136 27 27 ILE HB H 1.56 0.02 1 137 27 27 ILE HG12 H 1.19 0.02 1 138 27 27 ILE HG13 H 0.92 0.02 1 139 28 28 LYS H H 8.02 0.02 1 140 28 28 LYS HA H 3.81 0.02 1 141 28 28 LYS HB2 H 1.88 0.02 1 142 28 28 LYS HB3 H 1.90 0.02 1 143 28 28 LYS HE2 H 3.02 0.02 1 144 28 28 LYS HE3 H 3.02 0.02 1 145 29 29 GLU H H 8.02 0.02 1 146 29 29 GLU HA H 4.17 0.02 1 147 29 29 GLU HB2 H 1.88 0.02 1 148 29 29 GLU HB3 H 1.90 0.02 1 149 29 29 GLU HG2 H 2.36 0.02 1 150 29 29 GLU HG3 H 2.46 0.02 1 151 30 30 VAL H H 6.96 0.02 1 152 30 30 VAL HA H 3.96 0.02 1 153 30 30 VAL HB H 2.91 0.02 1 154 30 30 VAL HG1 H 1.49 0.02 1 155 30 30 VAL HG2 H 1.22 0.02 1 stop_ save_